Jeong, Eun Ju
(Department of Agronomy & Medicinal Plant Resources, Gyeongnam National University of Science and Technology)
,
Lee, Mi Kyeong
(College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University)
,
Kim, Young Choong
(College of Pharmacy, Chungbuk National University)
,
Sung, Sang Hyun
(College of Pharmacy, Chungbuk National University)
Activity-guided isolation to search for antifibrotic compounds from natural products using HSC-T6 cells afforded nine flavonoids or phenolics, luteolin (1), 3'-O-methylorobol (2), acactin 7-rutinoside (3), sedelolactone (4), 4-methoxyphenol (5), 4-hydroxyaldehyde (6), 4-hydoxyaldehyde (7), 4-hydroxy...
Activity-guided isolation to search for antifibrotic compounds from natural products using HSC-T6 cells afforded nine flavonoids or phenolics, luteolin (1), 3'-O-methylorobol (2), acactin 7-rutinoside (3), sedelolactone (4), 4-methoxyphenol (5), 4-hydroxyaldehyde (6), 4-hydoxyaldehyde (7), 4-hydroxy-3-methoxybenzoic acid (8), and ferulic acid (9) from the methanolic extract of aerial parts of Eclipta prostrata L.. Among the isolated compounds, luteolin (1) significantly inhibited the proliferation of HSCs in dose- and time-dependent manners. Antifibrotic activity of E. prostrata and its phenolic compounds might provide potential therapeutical choice in the treatment of hepatic fibrosis.
Activity-guided isolation to search for antifibrotic compounds from natural products using HSC-T6 cells afforded nine flavonoids or phenolics, luteolin (1), 3'-O-methylorobol (2), acactin 7-rutinoside (3), sedelolactone (4), 4-methoxyphenol (5), 4-hydroxyaldehyde (6), 4-hydoxyaldehyde (7), 4-hydroxy-3-methoxybenzoic acid (8), and ferulic acid (9) from the methanolic extract of aerial parts of Eclipta prostrata L.. Among the isolated compounds, luteolin (1) significantly inhibited the proliferation of HSCs in dose- and time-dependent manners. Antifibrotic activity of E. prostrata and its phenolic compounds might provide potential therapeutical choice in the treatment of hepatic fibrosis.
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제안 방법
Results are expressed as the mean ± SD of three independent experiments, each performed using triplicate wells.
TLC and column chromatography (CC) were carried out on precoated silica gel F254 plates (art. 5715, Merck), RP-18 F254 plates (art. 15423, Merck), silica gel 60 (230 - 400 mesh, Merck), Sephadex LH 20 (18 - 110 µm, Pharmacia Co. Ltd.) and Diaion HP-20 (250 - 850 µm, Mitshubishi Chemical).
이론/모형
) was used as a positive control. Cell viability was assessed by the 3-(4,5- dimethylthiazol-2-yl)-2.5-diphenyltetrazolium bromide (MTT) assay. HSC-T6 cells were incubated with 0.
HSC-T6 cells were incubated with each compound tested at the concentration of 100 µM for 48 h. Cell viability was measured by the MTT assay. The percent cell viability (%) was calculated as 100 × (absorbance of compound treated/absorbance of control).
성능/효과
− Compounds to be tested were dissolved in dimethylsulfoxide (DMSO). Our preliminary study showed that DMSO at a final concentration of 0.1% in media did not affect the cell viability. HSC-T6 cells were treated with vehicle or compounds to be tested for 48 h or as indicated.
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