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NTIS 바로가기Journal of embryo transfer = 한국수정란이식학회지, v.28 no.3, 2013년, pp.289 - 295
오건봉 (농촌진흥청 국립축산과학원 동물바이오공학과) , 김벨라 (농촌진흥청 국립축산과학원 동물바이오공학과) , 황성수 (농촌진흥청 국립축산과학원 동물바이오공학과) , 옥선아 (농촌진흥청 국립축산과학원 동물바이오공학과) , 임석기 (농촌진흥청 국립축산과학원 동물바이오공학과) , 박진기 (농촌진흥청 국립축산과학원 동물바이오공학과)
Xenotransplantation of pig organs into primates results in fatal damage, referred as hyperacute rejection (HAR), and acute humoral xenograft rejection (AHXR), to the organ graft mediated by antibodies pre-existing and newly-producing in primates against their cognate pig antigens. Functional ablatio...
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핵심어 | 질문 | 논문에서 추출한 답변 |
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말기 단계의 장기 부전 환자 치료법 중 가장 좋은 해결책은? | 말기 단계의 장기 부전 환자 치료법으로 가장 좋은 해결책은 장기 이식이다. 그렇지만 장기 이식을 대기하는 수여자에 비해 공여자의 수가 극히 적어 이종의 장기를 이식하는 이종이식이 대체 방법의 하나로 제시되고 있다. | |
이종이식이 장기 이식의 대체 방법으로 제시되는 이유는? | 말기 단계의 장기 부전 환자 치료법으로 가장 좋은 해결책은 장기 이식이다. 그렇지만 장기 이식을 대기하는 수여자에 비해 공여자의 수가 극히 적어 이종의 장기를 이식하는 이종이식이 대체 방법의 하나로 제시되고 있다. 돼지는 사람과 비교했을 때, 그 생리적 특성과 장기의 크기 등 여러 관점에서 대체 장기를 제공하기 위한 가장 적당한 재료로 인식되고 있다. | |
영장류에 돼지의 장기를 이식하면 초급성 거부 반응이 발생하는 이유는? | 사람과 유사한 면역학적 특성을 보유한 영장류에 돼지의 장기를 이식하면 몇 분에서 몇 시간 안에 이식된 장기가 손상되면서 폐사하게 되는 초급성 거부 반응이 발생한다(Yang과 Sykes, 2007). 이 거부 반응은 사람 및 영장류에는 존재하지 않는 돼지의 Gala1-3Galb1-4GlcNAc-R(Gal) 항원과 이에 대한 사람 및 영장류에 존재하는 Gal 자연항체의 면역 반응에 의해 발생한다(Macher와 Galili, 2008). 그런데 Gal 항원이 제거된 돼지의 장기를 영장류에 이식하면 초급성 거부 반응은 제어되지만, 여전히 급성 체액성(acute humoral rejection)이라 불리는 면역 거부 반응이 발생한다. |
Adams DH, Kadner A, Chen RH and Farivar RS. 2001. Human membrane cofactor protein (MCP, CD 46) protects transgenic pig hearts from hyperacute rejection in primates. Xenotransplantation 8: 36-40.
Ahn KS, Kim YJ, Kim M, Lee BH, Heo SY, Kang MJ, Kang YK, Lee JW, Lee KK, Kim JH, Nho WG, Hwang SS, Woo JS, Park JK, Park SB and Shim H. 2011. Resurrection of an alpha-1,3-galactosyltransferase gene-targeted miniature pig by recloning using postmortem ear skin fibroblasts. Theriogenology 75: 933-939.
Chen G, Qian H, Starzl T, Sun H, Garcia B, Wang X, Wise Y, Liu Y, Xiang Y, Copeman L, Liu W, Jevnikar A, Wall W, Cooper DK, Murase N, Dai Y, Wang W, Xiong Y, White DJ and Zhong R. 2005. Acute rejection is associated with antibodies to non-Gal antigens in baboons using Galknockout pig kidneys. Nat. Med. 11: 1295-1298.
Dai Y, Vaught TD, Boone J, Chen SH, Phelps CJ, Ball S, Monahan JA, Jobst PM, McCreath KJ, Lamborn AE, Cowell-Lucero JL, Wells KD, Colman A, Polejaeva IA and Ayares DL. 2002. Targeted disruption of the alpha 1,3-galactosyltransferase gene in cloned pigs. Nat. Biotechnol. 20: 251-255.
Davila E, Byrne GW, LaBreche PT, McGregor HC, Schwab AK, Davies WR, Rao VP, Oi K, Tazelaar HD, Logan JS and McGregor CG. 2006. T-cell responses during pig-toprimate xenotransplantation. Xenotransplantation 13: 31-40.
Dickens S, Van den Berge S, Hendrickx B, Verdonck K, Luttun A and Vranckx JJ. 2010. Nonviral transfection strategies for keratinocytes, fibroblasts, and endothelial progenitor cells for ex vivo gene transfer to skin wounds. Tissue Eng. Part C Methods 16: 1601-1608.
Ekser B, Klein E, He J, Stolz DB, Echeverri GJ, Long C, Lin CC, Ezzelarab M, Hara H, Veroux M, Ayares D, Cooper DK and Gridelli B. 2012. Genetically-engineered pig-tobaboon liver xenotransplantation: histopathology of xenografts and native organs. PloS one 7: e29720.
Ekser B, Long C, Echeverri GJ, Hara H, Ezzelarab M, Lin CC, de Vera ME, Wagner R, Klein E, Wolf RF, Ayares D, Cooper DK and Gridelli B. 2010. Impact of thrombocytopenia on survival of baboons with genetically modified pig liver transplants: clinical relevance. Am. J. Transplant. 10: 273-285.
Ekser B, Rigotti P, Gridelli B and Cooper DK. 2009. Xenotransplantation of solid organs in the pig-to-primate model. Transpl. Immunol. 21: 87-92.
Fodor WL, Williams BL, Matis LA, Madri JA, Rollins SA, Knight JW, Velander W and Squinto SP. 1994. Expression of a functional human complement inhibitor in a transgenic pig as a model for the prevention of xenogeneic hyperacute organ rejection. Proc. Natl. Acad. Sc.i U. S. A. 91: 11153-11157.
Gresch O, Engel FB, Nesic D, Tran TT, England HM, Hickman ES, Korner I, Gan L, Chen S, Castro-Obregon S, Hammermann R, Wolf J, Muller-Hartmann H, Nix M, Siebenkotten G, Kraus G and Lun K. 2004. New non-viral method for gene transfer into primary cells. Methods 33: 151-163.
Harrison SJ, Guidolin A, Faast R, Crocker LA, Giannakis C, D'Apice AJ, Nottle MB and Lyons I. 2002. Efficient generation of alpha(1,3) galactosyltransferase knockout porcine fetal fibroblasts for nuclear transfer. Transgenic Res. 11: 143-150.
Hauschild J, Petersen B, Santiago Y, Queisser AL, Carnwath JW, Lucas-Hahn A, Zhang L, Meng X, Gregory PD, Schwinzer R, Cost GJ and Niemann H. 2011. Efficient generation of a biallelic knockout in pigs using zinc-finger nucleases. Proc. Natl. Acad. Sc.i U. S. A. 108: 12013-12017.
Klymiuk N, Aigner B, Brem G and Wolf E. 2010. Genetic modification of pigs as organ donors for xenotransplantation. Mol. Reprod. Dev. 77: 209-221.
Ko N, Lee JW, Hwang SS, Kim B, Ock SA, Lee SS, Im GS, Kang MJ, Park JK, Jong Oh S and Bong Oh K. 2013. Nucleofection- mediated alpha 1,3-galactosyltransferase gene inactivation and membrane cofactor protein expression for pigto-primate xenotransplantation. Anim. Biotechnol. 24: 253-267.
Kolber-Simonds D, Lai L, Watt SR, Denaro M, Arn S, Augenstein ML, Betthauser J, Carter DB, Greenstein JL, Hao Y, Im GS, Liu Z, Mell GD, Murphy CN, Park KW, Rieke A, Ryan DJ, Sachs DH, Forsberg EJ, Prather RS and Hawley RJ. 2004. Production of alpha-1,3-galactosyltransferase null pigs by means of nuclear transfer with fibroblasts bearing loss of heterozygosity mutations. Proc. Natl. Acad. Sc.i U. S. A. 101: 7335-7340.
Lai L, Kolber-Simonds D, Park KW, Cheong HT, Greenstein JL, Im GS, Samuel M, Bonk A, Rieke A, Day BN, Murphy CN, Carter DB, Hawley RJ and Prather RS. 2002. Production of alpha-1,3-galactosyltransferase knockout pigs by nuclear transfer cloning. Science 295: 1089-1092.
Lin CC, Ezzelarab M, Shapiro R, Ekser B, Long C, Hara H, Echeverri G, Torres C, Watanabe H, Ayares D, Dorling A and Cooper DK. 2010. Recipient tissue factor expression is associated with consumptive coagulopathy in pig-to-primate kidney xenotransplantation. Am. J. Transplant. 10: 1556-1568.
Liszewski MK, Kemper C, Price JD and Atkinson JP. 2005. Emerging roles and new functions of CD46. Springer Semin. Immunopathol. 27: 345-358.
Londrigan SL, Brady JL, Sutherland RM, Hawthorne WJ, Thomas HE, Jhala G, Cowan PJ, Kay TW, O'Connell PJ and Lew AM. 2007. Evaluation of promoters for driving efficient transgene expression in neonatal porcine islets. Xenotransplantation 14: 119-125.
Macchiarini P, Oriol R, Azimzadeh A, de Montpreville V, Rieben R, Bovin N, Mazmanian M and Dartevelle P. 1998. Evidence of human non-alpha-galactosyl antibodies involved in the hyperacute rejection of pig lungs and their removal by pig organ perfusion. J. Thorac. Cardiovasc. Surg. 116: 831-843.
Macher BA and Galili U. 2008. The Galalpha1,3Galbeta1,4Glc-NAc-R (alpha-Gal) epitope: a carbohydrate of unique evolution and clinical relevance. Biochim. Biophys. Acta. 1780: 75-88.
McCreath KJ, Howcroft J, Campbell KH, Colman A, Schnieke AE and Kind AJ. 2000. Production of gene-targeted sheep by nuclear transfer from cultured somatic cells. Nature 405: 1066-1069.
Miyagawa S, Yamamoto A, Matsunami K, Wang D, Takama Y, Ueno T, Okabe M, Nagashima H and Fukuzawa M. 2010. Complement regulation in the Gal-T KO era. Xenotransplantation 17: 11-25.
Nakayama A, Sato M, Shinohara M, Matsubara S, Yokomine T, Akasaka E, Yoshida M and Takao S. 2007. Efficient transfection of primarily cultured porcine embryonic fibroblasts using the Amaxa nucleofection system. Cloning Stem Cells 9: 523-534.
Niemann H, Verhoeyen E, Wonigeit K, Lorenz R, Hecker J, Schwinzer R, Hauser H, Kues WA, Halter R, Lemme E, Herrmann D, Winkler M, Wirth D and Paul D. 2001. Cytomegalovirus early promoter induced expression of hCD59 in porcine organs provides protection against hyperacute rejection. Transplantation 72: 1898-1906.
Nottle MB, Beebe LF, Harrison SJ, McIlfatrick SM, Ashman RJ, O'Connell PJ, Salvaris EJ, Fisicaro N, Pommey S, Cowan PJ and d'Apice AJ. 2007. Production of homozygous alpha-1,3-galactosyltransferase knockout pigs by breeding and somatic cell nuclear transfer. Xenotransplantation 14: 339-344.
Petersen B, Ramackers W, Tiede A, Lucas-Hahn A, Herrmann D, Barg-Kues B, Schuettler W, Friedrich L, Schwinzer R, Winkler M and Niemann H. 2009. Pigs transgenic for human thrombomodulin have elevated production of activated protein C. Xenotransplantation 16: 486-495.
Phelps CJ, Ball SF, Vaught TD, Vance AM, Mendicino M, Monahan JA, Walters AH, Wells KD, Dandro AS, Ramsoondar JJ, Cooper DK and Ayares DL. 2009. Production and characterization of transgenic pigs expressing porcine CTLA4-Ig. Xenotransplantation 16: 477-485.
Phelps CJ, Koike C, Vaught TD, Boone J, Wells KD, Chen SH, Ball S, Specht SM, Polejaeva IA, Monahan JA, Jobst PM, Sharma SB, Lamborn AE, Garst AS, Moore M, Demetris AJ, Rudert WA, Bottino R, Bertera S, Trucco M, Starzl TE, Dai Y and Ayares DL. 2003. Production of alpha 1,3-galactosyltransferase-deficient pigs. Science 299: 411-414.
Ramirez P, Montoya MJ, Rios A, Garcia Palenciano C, Majado M, Chavez R, Munoz A, Fernandez OM, Sanchez A, Segura B, Sansano T, Acosta F, Robles R, Sanchez F, Fuente T, Cascales P, Gonzalez F, Ruiz D, Martinez L, Pons JA, Rodriguez JI, Yelamos J, Cowan P, d'Apice A and Parrilla P. 2005. Prevention of hyperacute rejection in a model of orthotopic liver xenotransplantation from pig to baboon using polytransgenic pig livers (CD55, CD59, and H-transferase). Transplant. Proc. 37: 4103-4106.
Ramsoondar JJ, Machaty Z, Costa C, Williams BL, Fodor WL and Bondioli KR. 2003. Production of alpha 1,3-galactosyltransferase-knockout cloned pigs expressing human alpha 1,2-fucosylosyltransferase. Biol. Reprod. 69: 437-445.
Sheyn D, Pelled G, Zilberman Y, Talasazan F, Frank JM, Gazit D and Gazit Z. 2008. Nonvirally engineered porcine adipose tissue-derived stem cells: use in posterior spinal fusion. Stem Cells 26: 1056-1064.
Skrzyszowska M, Samiec M, Slomski R, Lipinski D and Maly E. 2008. Development of porcine transgenic nuclear-transferred embryos derived from fibroblast cells transfected by the novel technique of nucleofection or standard lipofection. Theriogenology 70: 248-259.
Takahagi Y, Fujimura T, Miyagawa S, Nagashima H, Shigehisa T, Shirakura R and Murakami H. 2005. Production of alpha 1,3-galactosyltransferase gene knockout pigs expressing both human decay-accelerating factor and N-acetylglucosaminyltransferase III. Mol. Reprod. Dev. 71: 331-338.
White DJ, Cozzi E, Langford G, Oglesby T, Wang MW, Wright L and Wallwork J. 1995. The control of hyperacute rejection by genetic engineering of the donor species. Eye 9 (Pt 2): 185-189.
Yang YG and Sykes M. 2007. Xenotransplantation: current status and a perspective on the future. Nat. Rev. Immunol. 7: 519-531.
Zhou CY, McInnes E, Parsons N, Langford G, Lancaster R, Richards A, Pino-Chavez G, Dos Santos Cruz G, Copeman L, Carrington C and Thompson S. 2002. Production and characterization of a pig line transgenic for human membrane cofactor protein. Xenotransplantation 9: 183-190.
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