Purpose: Sulforaphane is a naturally-occurring isothiocyanate abundant in broccoli. It has been suggested as a promising antioxidant. In this study, the therapeutic effect of sulforaphane in paraquat intoxication was investigated. Methods: Paraquat was administered via the tail vein, after which sul...
Purpose: Sulforaphane is a naturally-occurring isothiocyanate abundant in broccoli. It has been suggested as a promising antioxidant. In this study, the therapeutic effect of sulforaphane in paraquat intoxication was investigated. Methods: Paraquat was administered via the tail vein, after which sulforaphane or a vehicle (4% DMSO) was administered intraperitoneally 15 minutes after paraquat administration. Histological injury, lipid peroxidation, plasma cytokine (IL-6, IL-10), and nitric oxide were measured. In addition, the effect of sulforaphane on survival in paraquat-intoxication was observed. Results: Regarding histological injury, lipid peroxidation, and plasma cytokine and nitric-oxide response, sulforaphane administration showed no protective effects in paraquat-intoxicated rats. Rather, it increased mortality (log rank p=0.03) and caused lipid peroxidation, as well as plasma cytokine and nitric-oxide production, to be increased. Conclusion: Sulforaphane had no therapeutic effect on paraquat-intoxicated rats; rather, it increased mortality.
Purpose: Sulforaphane is a naturally-occurring isothiocyanate abundant in broccoli. It has been suggested as a promising antioxidant. In this study, the therapeutic effect of sulforaphane in paraquat intoxication was investigated. Methods: Paraquat was administered via the tail vein, after which sulforaphane or a vehicle (4% DMSO) was administered intraperitoneally 15 minutes after paraquat administration. Histological injury, lipid peroxidation, plasma cytokine (IL-6, IL-10), and nitric oxide were measured. In addition, the effect of sulforaphane on survival in paraquat-intoxication was observed. Results: Regarding histological injury, lipid peroxidation, and plasma cytokine and nitric-oxide response, sulforaphane administration showed no protective effects in paraquat-intoxicated rats. Rather, it increased mortality (log rank p=0.03) and caused lipid peroxidation, as well as plasma cytokine and nitric-oxide production, to be increased. Conclusion: Sulforaphane had no therapeutic effect on paraquat-intoxicated rats; rather, it increased mortality.
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문제 정의
Second, we did not evaluate the effects of sulforaphane pre-treatment and other dose of sulforaphane on paraquat intoxication. This study was designed to evaluate the therapeutic potential of sulforaphane in paraquat intoxication. Thus, pretreatment was not considered initially and the dose of sulforaphane chosen in this study was derived by previous studies, which showed antioxidant effects of sulforaphane post-treatment.
Thus, the objective of this study is to examine the therapeutic effects of sulforaphane in paraquat intoxication in rats.
가설 설정
Thus, further study will be needed. Third, we did not evaluate the sole effects of sulforaphane. Sham-operated animal experiments will be needed to clarify whether sulforaphane has anti-oxidant or pro-oxidant effects in this model.
제안 방법
In this study, we administered sulforaphane 15 minutes after paraquat injection for testing the therapeutic effects. However, we did not find any protective effects of sulforaphane in paraquat intoxication model of rats.
Two sets of experiment were performed to examine the therapeutic effects of sulforaphane in paraquat intoxication.
대상 데이터
This study was approved by the Animal Care and Use Committee of our institute and was conducted in accordance with National Institute of Health guidelines. Male Sprague-Dawley rats (Orient Bio, Seoul, Korea) weighing 300~350 g were used for experiment. The rats were housed in a controlled environment with free access to food and water for one week before the experiment.
데이터처리
Student’s t-test and Mann-Whitney U test were performed as appropriate. Survival rates were compared by Kaplan-Meier log-rank test. Statistical analyses were conducted using STATA 10.
성능/효과
Sham-operated animal experiments will be needed to clarify whether sulforaphane has anti-oxidant or pro-oxidant effects in this model. Fourth, anti-oxidants like NQO1, HO-1 or glutathione and ROS were not measured. Thus, the pro-oxidant effects of sulforaphane could not explain increased mortality in sulforaphane group.
First, we did not conclude whether sulforaphane in paraquat intoxication might be beneficial or harmful by this small animal study. In this study, we did not Protective Effects in Paraquat-intoxicated Rats reach the significant result on the effects of sulforaphane in paraquat intoxication except mortality. This might be derived from early harvest of tissues.
The results of this study demonstrate that sulforaphane post-treatment did not show beneficial effect in paraquat intoxication. Rather sulforaphane increased mortality, and lipid peroxidation, inflammatory cytokine expression and NO production was not significantly different but showed a tendency to be increased in sulforaphane group.
후속연구
(29) However, there is no report about the role of iron chelation in the anti-oxidant effect of sulforaphane. Further study will be needed to elucidate the pro-oxidant or anti-oxidant effects of sulforaphane.
Thus, pretreatment was not considered initially and the dose of sulforaphane chosen in this study was derived by previous studies, which showed antioxidant effects of sulforaphane post-treatment. Thus, further study will be needed. Third, we did not evaluate the sole effects of sulforaphane.
If sulforaphane will be administered before the paraquat intoxication, this may have protective effects. To clarify this, further studies will be needed.
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