Ultraviolet (UV) irradiation alters multiple molecular pathways in the skin, thereby inducing skin damage, including photoaging. In recent years, probiotics have gained interest due to their beneficial effects on skin health, such as inhibiting atopic dermatitis and improving skin immunity or inflam...
Ultraviolet (UV) irradiation alters multiple molecular pathways in the skin, thereby inducing skin damage, including photoaging. In recent years, probiotics have gained interest due to their beneficial effects on skin health, such as inhibiting atopic dermatitis and improving skin immunity or inflammation. However, little is known about the effects of probiotics on UVB-induced photoaging. In this study, we evaluated the effect of Lactobacillus plantarum HY7714 against UVB-induced photoaging in human dermal fibroblasts and hairless mice. The results showed that L. plantarum HY7714 treatment effectively rescued UVB-reduced procollagen expression through the inhibition of UVB-induced matrix metalloproteinase (MMP)-1 expression in human dermal fibroblasts. Data from a western blot showed that L. plantarum HY7714 inhibited the phosphorylation of Jun N-terminal kinase, thereby suppressing the UVB-induced phosphorylation and expression of c-Jun. Oral administration of L. plantarum HY7714 clearly inhibited the number, depth, and area of wrinkles in hairless mouse skin. Histological data showed that L. plantarum HY7714 significantly inhibited UVB-induced epidermal thickness in mice. Western blot and zymography data also revealed that L. plantarum HY7714 effectively inhibited MMP-13 expression as well as MMP-2 and -9 activities in dermal tissue. Collectively, these results provide further insight regarding the skin biological actions of L. plantarum HY7714, a potential skin anti-photoaging agent.
Ultraviolet (UV) irradiation alters multiple molecular pathways in the skin, thereby inducing skin damage, including photoaging. In recent years, probiotics have gained interest due to their beneficial effects on skin health, such as inhibiting atopic dermatitis and improving skin immunity or inflammation. However, little is known about the effects of probiotics on UVB-induced photoaging. In this study, we evaluated the effect of Lactobacillus plantarum HY7714 against UVB-induced photoaging in human dermal fibroblasts and hairless mice. The results showed that L. plantarum HY7714 treatment effectively rescued UVB-reduced procollagen expression through the inhibition of UVB-induced matrix metalloproteinase (MMP)-1 expression in human dermal fibroblasts. Data from a western blot showed that L. plantarum HY7714 inhibited the phosphorylation of Jun N-terminal kinase, thereby suppressing the UVB-induced phosphorylation and expression of c-Jun. Oral administration of L. plantarum HY7714 clearly inhibited the number, depth, and area of wrinkles in hairless mouse skin. Histological data showed that L. plantarum HY7714 significantly inhibited UVB-induced epidermal thickness in mice. Western blot and zymography data also revealed that L. plantarum HY7714 effectively inhibited MMP-13 expression as well as MMP-2 and -9 activities in dermal tissue. Collectively, these results provide further insight regarding the skin biological actions of L. plantarum HY7714, a potential skin anti-photoaging agent.
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제안 방법
Since UVB irradiation induces the epidermis to become significantly thicker, we further evaluated the effect of L. plantarum HY7714 on UVB-induced epidermal thickening. To measure the thickness of the epidermis, biopsied skin specimens were stained with hematoxylin and eosin.
The animal protocol used in this study was reviewed and approved based on ethical procedures and scientific care by the Ethics Committee at the R&BD Center of the Korea Yakult Company Limited (KYIACUC-11-04-0231-Y). The mice were divided into a control group (n = 8), a UVB-only treatment group (n = 8), and a UVB plus L. plantarum HY7714 treatment group (n = 8). The mice in the control and UVB-only treatment groups were orally administered 100 µl of PB S.
(B) MMP-2/-9 activities were determined by zymography in mouse skin. The protein levels were quantified using an image analysis program to evaluate the density of each band on the immunoblot or zymography, and the fold-change value was calculated. Data are representative of three independent experiments.
Data are representative of three independent experiments. The protein levels were quantified using an image analysis program to evaluate the density of each band on the immunoblot, and the fold-change value was calculated.
However, only a few studies were designed to determine the anti-photoaging effects and mechanisms of probiotics on UVB-irradiated skin. Thus, the present study was designed to investigate the anti-photoaging activity and molecular mechanisms of Lactobacillus plantarum HY7714 on UVB-irradiated skin, both in vitro and in vivo.
MMP-1 expression is strongly regulated by the transcription factor AP-1, which is rapidly induced and activated by UV exposure in human skin in vivo. To examine this mechanism, we evaluated the expression and the phosphorylation of AP-1 subunits. The results showed that c-Jun phosphorylation and expression were significantly diminished after treatment with L.
plantarum HY7714 strongly reduced the wrinkle formation. To quantitatively analyze the number, area, and depth of wrinkles, we obtained the replicas from mouse dorsal skin at 12 weeks after UVB irradiation. The skin replicas were photographed and the three parameters were estimated using the skin VisioLine 650 system.
대상 데이터
Five-week-old female hairless mice were purchased from Central Lab Animal Inc. (Seoul, Korea). The mice were maintained in climate-controlled quarters (at 24℃, 50% humidity) with a 12 h light/12 h dark cycle.
Hs68 human dermal fibroblasts were purchased from the American Type Culture Collection (Manassas, VA, USA) and were cultured in monolayers at 37℃ in a 5% CO2 incubator in Dulbecco’s modified Eagle’s medium containing 10% fetal bovine serum.
데이터처리
Where appropriate, data are expressed as means ± SD values, and the Student’s t-test was used for multiple statistical comparisons.
성능/효과
To measure the thickness of the epidermis, biopsied skin specimens were stained with hematoxylin and eosin. The results showed that UVB-induced epidermal thickness of the dorsal skin was dramatically increased by 2.3-fold, due to chronic UVB exposure. On the other hand, oral administration of L.
To examine this mechanism, we evaluated the expression and the phosphorylation of AP-1 subunits. The results showed that c-Jun phosphorylation and expression were significantly diminished after treatment with L. plantarum HY7714 in Hs68 cells. However, L.
후속연구
In this study, we used live bacteria in the hairless mouse model. Thus, further studies are necessary to identify the probiotic components such as lipoteichoic acid or other metabolites responsible for the biological functions observed.
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