[논문]Effect of Oral Administration of Lactobacillus plantarum HY7714 on Epidermal Hydration in Ultraviolet B-Irradiated Hairless Mice

Ra, Jehyeon; Lee, Dong Eun; Kim, Sung Hwan; Jeong, Ji-Woong; Ku, Hyung Keun; Kim, Tae-Youl; Choi, Il-Dong; Jeung, Woonhee; Sim, Jae-Hun; Ahn, Young-Tae

doi:10.4014/jmb.1408.08023

Effect of Oral Administration of Lactobacillus plantarum HY7714 on Epidermal Hydration in Ultraviolet B-Irradiated Hairless Mice 원문보기

Journal of microbiology and biotechnology, v.24 no.12, 2014년, pp.1736 - 1743

Ra, Jehyeon (Korea Yakult Co., Ltd.) , Lee, Dong Eun (Korea Yakult Co., Ltd.) , Kim, Sung Hwan (Korea Yakult Co., Ltd.) , Jeong, Ji-Woong (Korea Yakult Co., Ltd.) , Ku, Hyung Keun (Korea Yakult Co., Ltd.) , Kim, Tae-Youl (Korea Yakult Co., Ltd.) , Choi, Il-Dong (Korea Yakult Co., Ltd.) , Jeung, Woonhee (Korea Yakult Co., Ltd.) , Sim, Jae-Hun (Korea Yakult Co., Ltd.) , Ahn, Young-Tae (Korea Yakult Co., Ltd.)

Abstract ▼ AI-Helper

In this study, we evaluated the effect of Lactobacillus plantarum HY7714 on skin hydration in human dermal fibroblasts and in hairless mice. In Hs68 cells, L. plantarum HY7714 not only increased the serine palmitoyltransferase (SPT) mRNA level, but also decreased the ceramidase mRNA level. In order to confirm the hydrating effects of L. plantarum HY7714 in vivo, we orally administered vehicle or L. plantarum HY7714 at a dose of $1{\times}10^9CFU/day$ to hairless mice for 8 weeks. In hairless mice, L. plantarum HY7714 decreased UVB-induced epidermal thickness. In addition, we found that L. plantarum HY7714 administration suppressed the increase in transepidermal water loss and decrease in skin hydration, which reflects barrier function fluctuations following UV irradiation. In particular, L. plantarum HY7714 administration increased the ceramide level compared with that in the UVB group. In the experiment on SPT and ceramidase mRNA expressions, L. plantarum HY7714 administration improved the reduction in SPT mRNA levels and suppressed the increase in ceramidase mRNA levels caused by UVB in the hairless mice skins. Collectively, these results suggest that L. plantarum HY7714 can be a potential candidate for preserving skin hydration levels against UV irradiation.

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가설 설정

A study on the gut–skin relationship reported that phenols produced by the gut bacteria accumulate in other organs, including the skin, in hairless mice and induce a skin disorder [16]. Thus, we hypothesized that oral administration of strain HY7714 may provide benefits to skin by having a positive influence on the intestinal microflora.

제안 방법

Based on the preliminary screening experiments for determining the levels of hyaluronic acid, SPT mRNA, and ceramidase mRNA in Hs68 cells, as shown in Fig. 1, L. plantarum HY7714 was selected as a candidate for consecutive studies among the various probiotics isolated from the feces of healthy infants or from breast milk. In a zequential UVB-irradiated hairless mice study, we investigated the dietary effects of L.

대상 데이터

Five-week-old female hairless mice were purchased from Central Lab Animal Inc. (Seoul, Korea). The mice were maintained in climate-controlled quarters (at 24℃, 55% relative humidity) with a 12 h light/12 h dark cycle.
Hs68 human dermal fibroblasts were purchased from the American Type Culture Collection (Manassas, USA) and were cultured as monolayers in Dulbecco’s modified Eagle’s medium containing 10% fetal bovine serum at 37℃ in a 5% CO2 incubator.

데이터처리

Where appropriate, data are expressed as the mean ± SEM, and the Student’s t-test was used for multiple statistical comparisons.

성능/효과

L. plantarum HY7714 administration increased STP mRNA levels by 2.74-fold (p < 0.05, Fig. 1B) and decreased ceramidase mRNA levels by 0.47-fold (p < 0.05, Fig. 1C) compared with that in the Con group, whereas the other lactic acid bacteria had no effects on both STP and ceramidase mRNA levels.

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