[논문]식물근권에서 분리한 Pseudomonas fluorescens strain EP103에 의한 고추역병억제

김택수; 스와나리더타; 이세원; 박경석

doi:10.7585/kjps.2014.18.4.422

식물근권에서 분리한 Pseudomonas fluorescens strain EP103에 의한 고추역병억제
Endophytic bacterium Pseudomonas fluorescens strain EP103 was effective against Phytophthora capsici causing blight in chili pepper 원문보기

농약과학회지 = The Korean journal of pesticide science, v.18 no.4, 2014년, pp.422 - 428

김택수 (농촌진흥청 국립농업과학원 농업미생물과) , 스와나리더타 (농촌진흥청 국립농업과학원 농업미생물과) , 이세원 (농촌진흥청 국립농업과학원 농업미생물과) , 박경석 (농촌진흥청 국립농업과학원 농업미생물과)

초록
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딸기 근권에서 분리한 내생균 중 고추역병균 방제 및 고추생육촉진 효과가 우수한 균주를 선발하였다. Pseudomonas fluorescen EP103으로 명명된 내생균주는 다른 내생균주와 비교하여 식물의 뿌리 길이와 생체 중이 크게 증가하였다. 고추역병에 대한 온실검정에서 EP103처리는 방제가 78.7%을 나타냈으며 항균력 실험결과 고추역병균을 직접 억제하지는 않았다. EP103이 처리된 고추에서는 PR1, PR10등의 병저항성 유전자가 발현되었으며 EP103의 PCR분석 결과 피올테오린, 파이로니트린, 하이드로젠 싸이아나이드, 오르화미드 등의 유용유전자를 함유하고 있음이 밝혀졌다. 따라서 본 균주는 고추역병의 생물 방제용으로 활용할 가치가 있는 것으로 판단된다.

Abstract ▼ AI-Helper

Endophytic bacterial strains from root tissue of strawberry were screened for their efficacy in growth improvement and control of Phytophthora blight disease of chili pepper plant under greenhouse condition. Plants treated with the strain EP103, identified as Pseudomonas fluorescens, showed growth improvement in terms of fresh weight and root length compared to the untreated control and other endophytic strains. When challenged with Phytophthora capsici, there was significant reduction of disease in EP103 treated plants with an efficacy of 78.7%. There was no direct inhibition of the target pathogen by EP103 when tested under in vitro antibiosis assay. Analysis of differential expression of selected marker genes for induced systemic resistance (ISR) in plants treated with EP103 and challenged with P. capsici showed up-regulation of PR1 and PR10 pathogenesis-related (PR) proteins. PCR analysis showed that EP103 produced secondary metabolites such as pyoluteorin, pyrrolnitrin, hydrogen cyanide and orfamide A. This study indicated the potential of endophytic P. fluorescens strain EP103 as an efficient biocontrol agent against P. capsici in chili pepper plant.

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제안 방법

fluorescens. The oligonucleotide primers developed from sequences available in NCBI for respective genes of P. fluorescens were used for this study. Lanes: (M) Ladder, (1) Pyoluteorin, (2) Pyrrolnitrin, (3) 2,4-diacetylphloroglucinol, (4) Hydrogen cyanide, (5) Orfamide A.

대상 데이터

The plates were incubated at 28°C for 7 days and inhibition zone was recorded in diameter (mm). Five replications were performed per treatment.
5% agarose gels at 80 V for 60 min. The experiments were conducted twice with three replicates (plants).
The fungal pathogen Phytophthora capsici was obtained from the Korean Agriculture Cultural Collection (KACC), National Academy of Agricultural Sciences (NAAS), Suwon, South Korea. The P.

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