[논문]Lignification in Relation to the Influence of Water-deficit Stress in Brassica napus

Lee, Bok-Rye; Zhang, Qian; Kim, Tae-Hwan

doi:10.5333/kgfs.2014.34.1.15

Lignification in Relation to the Influence of Water-deficit Stress in Brassica napus 원문보기

한국초지조사료학회지 = Journal of the Korean Society of Grassland and Forage Science, v.34 no.1, 2014년, pp.15 - 20

Lee, Bok-Rye (Department of Animal Science, Institute of Agriculture Science and Technology, College of Agriculture & Life Science, Chonnam National University) , Zhang, Qian (Department of Animal Science, Institute of Agriculture Science and Technology, College of Agriculture & Life Science, Chonnam National University) , Kim, Tae-Hwan (Department of Animal Science, Institute of Agriculture Science and Technology, College of Agriculture & Life Science, Chonnam National University)

Abstract ▼ AI-Helper

To investigate lignification process and its physiological significance under water-deficit condition, the responses of peroxidases, polyphenol oxidase (PPO) and phenylalanine ammonia-lyase (PAL) in relation to leaf water status to the short term of water deficit treatment in the leaves with different maturities in forage rape were measured. The significant decrease in relative water content (RWC) and leaf osmotic potential (${\Psi}{\pi}$) were apparent after 5 d of water-deficit treatment. The activity of guaiacol peroxidase (GPOD), ascorbate peroxidase (APOD), coniferyl alcohol peroxidase (CPOD), and syringaldazine peroxidase (SPOD) was depressed especially in middle and old leaves when compared with that of control leaves. On the other hand, in young leaves, a significant increase in CPOD (+34%) and SPOD (+24%) activity as affected by water-deficit treatment was apparent. The activation of PAL and PPO was observed in middle and old leaves for PAL and in young and middle leaves for PPO. These results suggest that peroxidases in middle and old leaves did not involve in lignification under mild water-deficit stress, whereas CPOD and SPOD in young leaves participate in lignification by a coordination with PAL and PPO to incorporate phenol and lignin into the cell walls.

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제안 방법

4). For the response of peroxidases activity to water-deficit treatment, the activity of four peroxidases examined in this study (GPOD, APOD, CPOD and SPOD) was depressed especially in middle and old leaves when compared with that of control leaves, respectively. In general, the isozymes of peroxidases participate in the modulation of cell wall properties during plant growth partly through catalyzing the formation of covalent crosslinks after oxidation of ester- and ether-bound phenolic acids and also through the oxidative coupling of cinnamoyl alcohol moieties to generate lignin (Iiyama et al.
, 2001), to drought treatment in forage rape plants. In this study, thus, the responses of peroxidases, PPO and PAL in relation to leaf water status to the short term of water deficit treatment in the leaves with different maturities in forage rape.

이론/모형

The amount of lignin was calculated from a linear calibration curve (0~40 μ g) built with commercial alkali lignin (Aldrich, Steinheim, Germany). Total phenol content was determined by FolinCiocalteau method (Singleton and Rossi, 1965). The phenolic contents were estimated using a standard curve using gallic acid.
Tukey’s studentized range test was used to compare the means of separate replicates.

성능/효과

In this study, 5 d of water-deficit treatment increased total phenol concentration by 48%, 39% and 73% (Fig. 4A), and lignin concentration by 51%, 15% and 22% (Fig. 4B), respectively, in young, middle and old leaves. These results indicate that lignification process in forage rape plants occurs, but that the responses of peroxidases and lignifying enzymes were different depending on leaf maturity.

후속연구

These results suggest that peroxidases in middle and old leaves did not involve in lignification under mild water-deficit stress, whereas CPOD and SPOD in young leaves participate in lignification by a coordination with PAL and PPO to incorporate phenol and lignin into the cell walls. The works further will be necessary to assess whether severe drought would be responsible for the structural rigidity and lignin incorporation in middle and old leaves, which lead to a reduction of growth and digestibility in forage rape plants.

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Lignification in Relation to the Influence of Water-deficit Stress in Brassica napus 원문보기

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Lignification in Relation to the Influence of Water-deficit Stress in Brassica napus 원문보기

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