[논문]Mycorrhiza 접종이 가뭄 스트레스하의 식물성장과 질소 대사산물에 미치는 영향

이복례; 정우진; 김대현; 김길용; 손보균; 김태환

Mycorrhiza 접종이 가뭄 스트레스하의 식물성장과 질소 대사산물에 미치는 영향
Effects of Mycorrhizal Inoculation on Plant Growth and N Metabolites in Relation to drought-stress Tolerance 원문보기

韓國土壤肥料學會誌 = Korean journal of soil science & fertilizer, v.35 no.5, 2002년, pp.314 - 325

이복례 (전남대학교 농업생명과학대학 동물자원학과) , 정우진 (전남대학교 농업생명과학대학 동물자원학과) , 김대현 (전남대학교 농업생명과학대학 동물자원학과) , 김길용 (전남대학교 농업생명과학대학 생물환경화학과) , 손보균 (순천대학교 농과대학 농화학과) , 김태환 (전남대학교 농업생명과학대학 동물자원학과 & 농업과학기술연구소)

초록
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페레니얼 라이그라스의 가뭄스트레스 저항성과 관련하여 균근균(mycorrhiza) 접종이 작물생장 및 질소대사 산물에 미치는 영향을 조사하였다. 균근균 접종에 의한 잎의 수분퍼텐셜, 건물량 및 인의 함량의 유의적인 증가가 인정되었다. 가뭄스트레스 상태에서 뿌리의 질산염 농도는 증가하였으며, 균근균 비접종구가 접종구에 비하여 현저하였다. 잎의 수용성 단백질 함량은 가뭄 스트레스 하에서 균근균 비접종구가 접종구에 비해 현저히 감소하였다. 전 실험기간 동안 가뭄 스트레스 처리구의 암모니아와 proline의 농도는 현저히 증가하였으며, 균근균 접종구에서는 증가가 현저히 완화되었다. 가뭄 스트레스 하에서 잎의 건물량 감소는 암모니아(p<0.01)와 proline(p<0.001) 농도의 증가와 부의 유의적인 상관성을 보였다. 이상의 결과로 볼 때, 균근균의 접종 효과는 가뭄 스트레스 하에서 작물의 성장 억제를 완화시켰으며, 인의 함량과 질소의 동화 작용을 증가 시켰다.

Abstract ▼ AI-Helper

The effects of arbuscular mycorrhizal (AM) fungus (Glomus intraradices) on plant growth and N metabolic responses were examined in perennial ryegrass plants exposed to drought-stressed or well-watered condition. Mycorrhizal inoculation improved significantly leaf water potential, dry mass and P content. Drought stress increased significantly nitrate concentration in roots where the increase was much less in AM than non-AM. Drought stress decreased the concentration of soluble proteins in non-AM shoots, whereas non-significant decline occurred in AM shoots even under drought condition. The concentrations of ammonia and proline in drought stressed non-AM plants significantly increased, while mycorrhizal inoculation lowered significantly ammonia and proline accumulation. The decrease in leaf dry weight in drought stressed-plants was significantly correlated to the increase in ammonia (p<0.01) and proline concentration (p<0.01). These results suggested that the increased P content and N assimilation by mycorrhizal inoculation may be associated with drought stress tolerance, showing the moderating effects on shoot growth inhibition and ammonia accumulation in drought stressed-plants.

주제어

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가설 설정

With these backgrounds, we hypothesized that AM inoculation to perennial ryegrass enhances N utilization that may be one of factors related to drought tolerance of host plant. Hence we examined the changes in the concentration of nitrogenous compounds in roots and shoots of AM or non-AM plants which had been subjected to drought condition.

제안 방법

The seeds washed with distilled water, and then germinated on wet filter paper for 7 days. Four treatment combinations [arbuscular mycorrhizal inoculation (AM) or non-AM inoculation (non-AM) under droughtstressed or well-watered condition] were replicated for three times in a randomized block design. The experimental soil containing 1% (w/w) rock phosphate and 3 % (w/w) organic matter was filled into 3L pots, and then sterilized.

데이터처리

A three-way analysis of variance (ANOVA) was applied to all data (SAS Institute Inc, 1989). Differences among the means of treatment within a sampling date were separated by least significant difierence (LSD) at the 5 % level of probability.

이론/모형

Proline was extracted and its concentration was determined by the method of Bates et al, (1973). Leaf seg ments were homogenized with 3 % sulfosalicylic acid and the homogenate was centrifuged at 3000 g for 20 min.
">reextracted. Soluble proteins in the combined supernatant were quantified by dye-binding method (Bradford, 1976).

성능/효과

P content in roots responded to drought and mycorrhizal treatment with a similar pattern observed of shoots. After 28 days of drought condition, root P content in non-AM plants decreased by 39 % but only 28 % in AM plants compare to wellwatered non-AM and AM plants.
In roots, a significant increase in ammonia concentration was observed at 14 days after treatment. After 28 days of drought treatment, the ammonia concentration in AM plant was lower (39.7 % in shoots and 18.1 % in roots) than non-AM plants, sueesting that mycorrhizal inoculation has a moderating influence on ammonia accumulation under drought stress (Fig. 3).
2 % at the beginning of the treatment. After 28 days of treatment, it increased to 84.25.9 % under wellwater condition, whereas it was almost constant (69.5 4.7 %) under drought condition (data not shown).
Rabe and Lovatt (1986) and Lazcano-Ferrat and Lovatt (1999) provided evidence those abiotic stresses that reduce leaf or shoot growth increase production of ammonia, leading to ammonia accumulation or its removal through biosynthesis of arginine, which is a precursor of proline (Stewart and Boggess, 1977). Consistent with these results, ammoina accumulation in drought stressed-plants was significantly (p<0.01) related to proline accumulation, but no direct relationship in well-watered plants. A similar founding has been reported in detached rice leaves (Yang and Kao, 2000).
The results of ANOVA showed that the soluble protein concentration in shoots and roots was significantly aflected by drought treatment (p<0.001) and mycorrhizal inoculation (pvO.Ol) ( Table 2). The concentration of soluble proteins in non-AM shoots under drought condition was significantly lower than other three treatments.

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