[논문]Anti-MRSA action of Papenfussiella kuromo

Lee, Sun-Ae; Mun, Su-Hyun; Kang, Ok-Hwa; Joung, Dae-Ki; Seo, Yun-Soo; Kang, Da-Hye; Kim, Sung-Bae; Kong, Ryong; Yang, Da-Wun; Kwon, Dong-Yeul

[국내논문] Anti-MRSA action of Papenfussiella kuromo 원문보기

Natural product sciences, v.20 no.1, 2014년, pp.39 - 43

Abstract ▼ AI-Helper

Papenfussiella kuromo (PK) is a marine plant and an abundant ecological resource for the future; it is found in almost 80% of the terrestrial biosphere. The aim of this study was to investigate the antibacterial activity of PK against methicillin-resistant Staphylococcus aureus (MRSA), multidrug-resistant pathogen. The minimum inhibitory concentrations (MICs) of PK hexane fraction (PKH) against 7 strains of MRSA ranged from 1.0 to 2.0 mg/mL. In the checkerboard dilution method, a synergistic effect of the PKH and the antibiotics (oxacillin and norfloxacin) was seen. PKH markedly reduced the MIC of each of the 4 antibiotics against MRSA. The time-kill assay showed that the synergistic activity of PKH and an antibiotic reduced the bacterial counts below the lowest detectable limit after 24 h. These findings suggest that PKH has antibacterial activity, and may be important baseline data in future extensive studies of living marine resources as a source of compounds active against MRSA.

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문제 정의

The study did show the PK was effective in treating MRSA infection. The result of antimicrobial efficacy of PK frantions against S.

제안 방법

In this study, we investigated the antimicrobial activity of Papenfussiella kuromo hexane fraction (PKH) against methicillin-resistant Staphylococcus aureus (MRSA). S.

대상 데이터

Bacterial strains and growth conditions − Among the 7 strains of MRSA used in this study, 2 were clinical isolates of MRSA obtained from 2 unique patients at Wonkwang University Hospital (Iksan, Korea).
The dried residue was then dissolved in dimethyl sulfoxide (DMSO) for further use. A papenfussiella kuromo sample was deposited at the Herbarium of the College of Pharmacy, Wonkwang University, Iksan.
Bacterial strains and growth conditions − Among the 7 strains of MRSA used in this study, 2 were clinical isolates of MRSA obtained from 2 unique patients at Wonkwang University Hospital (Iksan, Korea). S. aureus ATCC 33591 (methicillin-resistant strain), was purchased from the American Type Culture Collection (Manassas, VA). Methicillin-resistant bacteria CCARM 3090, CCARM 3091, CCARM 3095, and CCARM 3102 were provided by the Culture Collection of Antimicrobial Resistant Microbes (CCARM, Republic of Korea).
The diameter of the inhibition zone around each of the discs was measured and recorded at the end of the incubation period. The experiment was carried out in triplicate.

이론/모형

These bacterial cultures were suspended in MHB and incubated at 37 ℃ for 24 h. MHA was used in the agar diffusion method.
The study did show the PK was effective in treating MRSA infection. The result of antimicrobial efficacy of PK frantions against S. aureus strains was evaluated by examining surrounding inhibition zones in the paper disc diffusion method. The growth of each of the tested S.
The zone of inhibition increased in a concentration-dependent manner (this table has not shown). The antimicrobial susceptibility tests of the PKH against 7 strains of MRSA were performed using the broth dilution method, and the results are presented in Table 2. All the MRSA isolates were sensitive to the PKH.

성능/효과

Of the 14 combinations of organisms with antibiotics and PKH, 11 showed evidence of synergy, 1 of partial synergy, and 2 of an additive effect. When OXI and NOR were separately evaluated in combination with PKH, a significant decrease in the MIC of the antibacterial drugs against the MRSA strains was observed. For example, the MIC of OXI against DPS-1 decreased from 0.

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