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NTIS 바로가기Journal of plant biotechnology = 식물생명공학회지, v.41 no.1, 2014년, pp.1 - 9
김진영 (부산대학교 생명자원과학대학 식물생명과학과) , 이소의 (부산대학교 생명자원과학대학 식물생명과학과) , 오하람 (부산대학교 생명자원과학대학 식물생명과학과) , 최인수 (부산대학교 생명자원과학대학 식물생명과학과) , 김용철 (부산대학교 생명자원과학대학 식물생명과학과) , 김선태 (부산대학교 생명자원과학대학 식물생명과학과)
So far it has been generally considered that proteomic approaches are very useful for studying plant-microbes interaction. In this review, recent studies based on papers published from 2010 to 2013 have investigated proteomics analysis in various interaction during plant-fungal pathogen infection by...
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핵심어 | 질문 | 논문에서 추출한 답변 |
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단백질 정량분석의 한계를 극복하기 위한 여러 가지 대체 기술은? | 2006a, 2006b , 2008 , 2009, 2011). 하지만 최근에는 질량 분석기를 이용한 단백질 정량분석의 한계를 극복하기 위한 여러 가지 대체 기술(IC AT, SILAC, 2D-LC, ITRAQ )이 개발되고 있으며 또한 점차적으로 식물 및 병원균의 단백질체 연구에 많이 적용되고 있다(Lee and Koh, 2011). 하지만 아직도 고전적인 2-DE를 이용한 단백질체 연구는 꾸준히 지속되고 있다. | |
프로테오믹스에 있어 식물의 단백질을 분리해내는 것이 중요한 이유는? | 단백질을 용해하거나 추출하는 기술은 그 기술로부터 얻어낸 단백질을 이용하여 검정하고 동정하는 것을 가능하게 한다. 이것은 프로테오믹스에 있어 시료로부터 단백질을 분리해내는 것에 대한 중요성을 말해주며 식물을 재료로 하는 경우에 있어서 더 중요해 진다. | |
단백질 동정을 위한 질량 분석을 통해 알수 있는 것은? | 단백질 동정을 위한 질량 분석(M as s spectrometry, MS)은 단백질의 양질적 측면에서의 pro filin g 뿐만 아니라 단백질 종류를 동정하고 변화를 확인할 수 있게 해준다. 단백질체는 효소(트립신 등)나 화학적 처리를 통해 얻어지는 펩티드나 온전한 단백질의 질량 스펙트럼으로부터 동정 되며 그 종은 단백질, 유전체, EST s seq uen ce 또는 MS spectra 데이터베이스로부터 얻은 이론적인 것과 실험적 결과를 대조하여 동정될 수 있다(Gon zález-Fernández et al. |
Agrawal GK, Jwa NS, Iwahashi Y, Yonekura M (2006) Rejuvenating rice proteomics: facts, challenges, and visions. Proteomics 6:5549-5576
Agrawal GK, Rakwal R (2006) Rice Proteomics: a cornerstone for cereal food crop proteomes. Mass Spectrom Rev 25:1-53
Agrawal GK, Rakwal R (2008) Plant Proteomics: Technologies, Strategies, and Applications (Eds. GK Agrawal and R Rakwal). John Wiley & Sons, Inc., Hoboken, NJ, pp165-178
Agrawal GK, Jwa NS, Rakwal R (2009) Rice proteomics: ending phase I and the beginning of phase II. Proteomics 9:935-963
Agrawal GK, Jwa NS, Lebrun MH, Job D, Rakwal R (2010) Plant secretome: unlocking secrets of the secreted proteins. Proteomics 10:799-827
Agrawal GK, Rakwal R (2011) Rice proteomics: a move toward expanded proteome coverage to comparative and functional proteomics uncovers the mysteries of rice and plant biology. Proteomics 11:1630-1649
Edgar N, Vollmer M, Horth P, Vad C (2004) 2D-LC/MS techniques for the identification of proteins in highly complex mixtures. Expert Rev Proteomics 1:37-46
De Wolf ED, Effertz RJ, Ali S, Francl LJ (1998) Vistas of tan spot research. Can J Plant Pathol 20:349-370
Fang X, Chen W, Xin Y, Zhang H, Yan C, Yu H, Liu H, Xiao W, Wang S, Zheng G, Liu H, Jin L, Ma H, Ruan S (2012) Proteomic analysis of strawberry leaves infected with Colletorichum fragariae. J Proteomics 75:4074-4090
Fang X, Jost R, Finnegan PM, Barbetti MJ (2013) Comparative proteome analysis of the strawberry-Fusarium oxysporum f. sp. fragariae pathosystem reveals early activation of defense responses as a crucial determinant of host resis tance. J Proteome Res 12:1772-1788
Gonzalez-Fernandez R, Prats E, Jorrin-Novo JV (2010) Proteomics of plant pathogenic fungi. J Biomed Biotech 2010:1-36
Gunnaiah R, Kushalappa A, Duggavathi R, Fox S, Somers DJ (2012) Integrated metabolo-proteomic approach to decipher the mechanisms by which wheat QTL (Fhb1) contributes to resistance against Fusarium graminearum. PLoS ONE 7:e40695:1-15
Harder A (2008) Sample preparation procedure for cellular fungi. In 2D PAGE: Sample preparation and fractionation. Methods Mol Biol 425:265-273
Haynes PA, Roberts TH (2007) Subcellular shotgun proteomics in plants: looking beyond the usual suspects. Proteomics 16:2963-2975
Kalra A, Singh HB, Patra NK, Pandey R, Shukla RS, Kumar S (2001) The effect of leaf spot, rust and powdery mildew on yield components of nine Japanese mint (Mentha arvensis) genotypes. J Hort Sci Biotech 76:546-548
Kim ST, Cho KS, Jang YS, Kang KY (2001) Two-dimensional electrophoretic analysis of rice proteins by polyethylene glycol fractionation for protein arrays. Electrophoresis 22:2103-2109
Kim ST, Kim SG, Hwang du H, Kang SY, Kim HJ, Lee BH, Lee JJ, Kang KY (2004) Proteomic analysis of pathogen-responsive proteins from rice leaves induced by rice blast fungus, Magnaporthe grisea. Proteomics 4:3569-3578
Kim SG, Wang Y, Lee KH, Park ZY, Park J, Wu J, Kwon SJ, Lee YH, Agrawal GK, Rakwal R, Kim ST, Kang KY (2013) In-depth insight into in vivo apoplastic secretome of rice-Magnaporthe oryzae interaction. J Proteomics 78:58-71
Kim ST, Kim SG, Agrawal GK, Kikuchi S, Rakwal R (2014) Rice proteomics: A model system for crop improvement and food security. Proteomics 14:593-610
Kim YM, Bouras N, Kav NNV, Strelkov SE (2010) Inhibition of photosynthesis and modification of the wheat leaf proteome by Ptr ToxB: A host-specific toxin from the fungal pathogen Pyrenophora tritici-repentis. Proteomics 10:2911-2926
Koga H, Dohi K, Nishiuchi T, Kato T, Takahara H, Mori M, Komatsu S (2012) Proteomic analysis of susceptible rice plants expressing the whole plant-specific resistance against Magnaporthe oryzae: Involvement of a thaumatin-like protein. Physiol Mol Plant Pathol 77:60-66
Kosova K, Vitamvas P, Prasil IT, Renaut J (2011) Plant proteome changes under abiotic stress - Contribution of proteomics studies to understanding plant stress response. Proteomcis 74:1301-1322
Lee J, Koh HJ (2011) A label-free quantitative shotgun proteomics analysis of rice grain development. Proteome Sci 9:61
Li H, Goodwin PH, Han Q, Huang L, Kang Z (2012) Microscopy and proteomic analysis of the non-host resistance of Oryza sativa to the wheat leaf rust fungus, Puccinia triticina f. sp. tritici. Plant Cell Rep 31:637-650
Lilley KS, Dupree P (2007) Plant organelle proteomics. Curr Opin Plant Biol 10:594-599
Liu J, Wang X, Mitchell T, Hu Y, Liu X, Dai L (2010) Recent progress and understanding of the molecular mechanisms of the rice-Magnaporthe oryzae interaction. Mol Plant Pathol 11:419-427
Marra R, Li H, Barbetti MJ, Sivasithamparam K, Vinale F, Cavallo P, Lorito M (2010) Proteomic analysis of the interaction between Brassica napus cv. Surpass 400 and virulent or avirulent isolates of Leptosphaeria maculans. J Plant Pathol 92:89-101
Maytalman D, Mert Z, Baykal AT, Inan C, Gunel A, Hasancebi S (2013) Proteomic analysis of early responsive resistance proteins of wheat (Triticum aestivum) to yellow rust (Puccinia striiformis f. sp. tritici) using ProteomLab PF2D. J Plant Omics 6:24-35
McDonald T, Sheng S, Stanley B, Chen D, Ko Y, Cole RN, Pedersen P, Van EJE (2006) Expanding the subproteome of the inner mitochondria using protein separation technologies: one- and two-dimensional liquid chromatography and twodimensional gel electrophoresis. Mol Cell Proteomics 5:2392-2411
Medina ML, Francisco WA (2008) Isolation and enrichment of secreted proteins from filamentous fungi. In 2D PAGE: Sample preparation and fractionation. Methods Mol Biol 425:275-285
Pitarch A, Nombela C, Gil C (2008) Cell wall fractionation for yeast and fungal proteomics. Method in Molecular Biology 425:217-239
Qi X, Niks RE, Stam P, Lindhout P (1998) Identification of QTLs for partial resistance to leaf rust (Puccinia horden) in barley. Theor Appl Genet 96:1205-1215
Rampitsch C, Bykova NV, McCallum B, Beimcik (2006) Analysis of the wheat and Puccinia triticina (leaf rust) proteomes during a susceptible hostpathogen interaction. Proteomics 6:1897-1907
Rampitsch C, Bykova NV (2012) Proteomics and plant disease: advances in combating a major threat to the global food supply. Proteomics 12: 673-690
Saravanan RS, Rose JK (2004) A critical evaluation of sample extraction techniques for enhanced proteomic analysis of recalcitrant plant tissues. Proteomics 4:2522-2532
Shah P, Powell ALT, Orlando R, Bergmann C, Gutierrez-Sanchez G (2012) Proteomic analysis of ripening tomato fruit infected by Botrytis cinerea. J Proteome Res 11:2178-2192
Shenton MR, Berberich T, Kamo M, Yamashita T, Taira H, Terauchi R (2012) Use of intercellular washing fluid to investigate the secreted proteome of the rice-Magnaporthe interaction. J Plant Res 125:311-316
Sinha R, Chattopadhyay S (2011) Changes in the leaf proteome profile of Mentha arvensis in response to Alternaria alternata infection. J Proteomics 74:327-336
Sreenivasaprasad S, Talhinhas P. (2005) Genotypic and phenotypic diversity in Colletotrichum acutatum, a cosmopolitan pathogen causing anthracnose on a wide range of hosts. Mol Plant Pathol 6:361-378
Strelkov SE, Lamari L (2003) Host-parasite interactions in tan spot [Pyrenophora tritici-repentis] of wheat. Can J Plant Pathol 25:339-349
Talbot NJ (2003) On the trail of a cereal killer: Exploring the biology of Magnaporthe grisea. Annu Rev Microbiol 57:177-202
Tan KC. Ipcho SV, Trengove RD, Oliver P, Solomon PS (2009) Assessing the impact of transcriptomics proteomics and metabolomics on fungal phytopathology. Mol Plant Pathol 10:703-715
Tzorzakis N, Taybi T, Antony E, Singleton I, Borland A, Barnes J (2013) Profiling shifts in protein complement in tomato fruit induced by atmpospheric ozone-enrichment and/or wound-inoculation with Botrytis cinerea. Postharvest Biol Tech 78:67-75
Zhou W, Eudes F, Laroche A (2006) Identification of differentially regulated proteins in response to a compatible interaction between the pathogen Fusarium graminearum and its host, Triticum aestivum. Proteomics 6:4599-4609
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