Sleep is vital to maintain health and well-being; however, insomnia is currently a widespread health complaint worldwide. In particular, caffeine, a psychoactive component of coffee, tea, and caffeine beverages may lead to sleep disorders such as insomnia. In this study, our primary objective was to...
Sleep is vital to maintain health and well-being; however, insomnia is currently a widespread health complaint worldwide. In particular, caffeine, a psychoactive component of coffee, tea, and caffeine beverages may lead to sleep disorders such as insomnia. In this study, our primary objective was to investigate the inhibitory effect of high-purity phlorotannin preparation (HP-PRT) on caffeine-induced wakefulness. The sleep test of pentobarbital-induced mice was used as an in vivo animal model. Caffeine (50 and 100 mg/kg) showed significant arousal effects (an increase in sleep latency and a decrease in sleep duration). Co-administration of caffeine (50 mg/kg) and the sedative-hypnotic diazepam (DZP, 1 mg/kg) did not result in similar arousal activity. HP-PRT (500 mg/kg) also inhibited caffeine-induced wakefulness. Our results suggest that HP-PRT would be a useful additive for developing coffee products without the arousal effect.
Sleep is vital to maintain health and well-being; however, insomnia is currently a widespread health complaint worldwide. In particular, caffeine, a psychoactive component of coffee, tea, and caffeine beverages may lead to sleep disorders such as insomnia. In this study, our primary objective was to investigate the inhibitory effect of high-purity phlorotannin preparation (HP-PRT) on caffeine-induced wakefulness. The sleep test of pentobarbital-induced mice was used as an in vivo animal model. Caffeine (50 and 100 mg/kg) showed significant arousal effects (an increase in sleep latency and a decrease in sleep duration). Co-administration of caffeine (50 mg/kg) and the sedative-hypnotic diazepam (DZP, 1 mg/kg) did not result in similar arousal activity. HP-PRT (500 mg/kg) also inhibited caffeine-induced wakefulness. Our results suggest that HP-PRT would be a useful additive for developing coffee products without the arousal effect.
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제안 방법
5% CMC-saline, 10 ml/kg) were tested in parallel with the animals receiving test sample treatment. All experiments were performed between 13:00 and 17:00, and mice were fasted for 24 h prior to the experiment. Test solutions were administered (post-oral injection, p.
(2005), caffeine significantly produced arousal activity in analysis of sleep-wake profile based on electroencephalography and electromyography. In this study, 50 mg/kg was selected as a dosage of caffeine for the experiment to test the inhibitory effects of phlorotannins. A dosage of HP-PRT was determined by testing four concentrations of 62.
Regardless of molecular mechanisms of sleep-wake regulation, hypnotic or arousal compounds can accelerate or decelerate the pentobarbital-induced sleep. In this study, arousal effect of caffeine via the adenosine A2A receptor was offset by hypnotic effect of DZP and HP-PRT via the benzodiazepine site of the GABAA receptor. Therefore, this offset did not alter duration and latency of the pentobarbital-induced sleep.
Although a large amount of caffeine intake is known to lead to sleep disorders such as insomnia, few studies have been conducted on foods and their constituents with inhibitory effects on caffeine-induced wakefulness. In this study, we evaluated the effect of HP-PRT with sedative-hypnotic activity on caffeine-induced wakefulness by using the pentobarbitalinduced sleep test in mice. This method is useful to evaluate in vivo hypnotic and arousal activities (Ma et al.
The animals were housed in an insulated, soundproof recording room maintained at an ambient temperature of 23 ± 0.5°C, with a relative humidity (55 ± 2%) on an automatically controlled 12-h light/12-h dark cycle (lights on at 5:00).
To determine a statistically significant dosage of caffeine, its effects on sleep latency and sleep duration in mice were evaluated. With a hypnotic dose of pentobarbital (42 mg/kg), caffeine (12.
대상 데이터
ICR mice (male; 23–28 g; 4 weeks) were purchased from Koatech Animal Inc. (Pyeongtaek, Korea).
데이터처리
Comparisons between group data were analyzed using an unpaired Student’s t-test.
For multiple comparisons in the pentobarbital-induced sleep test, data were analyzed using one-way ANOVA followed by Dunnett’s test.
후속연구
Therefore, HP-PRT may be a good additive for the preparation of coffee products without an arousal effect. Further studies are needed to evaluate the effects of co-administration of HP-PRT and caffeine on sleep-wake architecture and profile.
참고문헌 (33)
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