[논문]Hepatoprotective Effect of Aged Black Garlic Extract in Rodents

Shin, Jung Hyu; Lee, Chang Woo; Oh, Soo Jin; Yun, Jieun; Kang, Moo Rim; Han, Sang-Bae; Park, Heungsik; Jung, Jae Chul; Chung, Yoon Hoo; Kang, Jong Soon

doi:10.5487/tr.2014.30.1.049

Hepatoprotective Effect of Aged Black Garlic Extract in Rodents 원문보기

Toxicological research, v.30 no.1, 2014년, pp.49 - 54

Shin, Jung Hyu (Bioevaluation Center, Korea Research Institute of Bioscience and Biotechnology) , Lee, Chang Woo (Bioevaluation Center, Korea Research Institute of Bioscience and Biotechnology) , Oh, Soo Jin (Bioevaluation Center, Korea Research Institute of Bioscience and Biotechnology) , Yun, Jieun (Bioevaluation Center, Korea Research Institute of Bioscience and Biotechnology) , Kang, Moo Rim (Bioevaluation Center, Korea Research Institute of Bioscience and Biotechnology) , Han, Sang-Bae (College of Pharmacy, Chungbuk National University) , Park, Heungsik (Novarex Co., Ltd.) , Jung, Jae Chul (Novarex Co., Ltd.) , Chung, Yoon Hoo (Namhaegun Blackgarlic Co., Ltd) , Kang, Jong Soon (Bioevaluation Center, Korea Research Institute of Bioscience and Biotechnology)

Abstract ▼ AI-Helper

In this study, we investigated the hepatoprotective effects of aged black garlic (ABG) in rodent models of liver injury. ABG inhibited carbon tetrachloride-induced elevation of aspartate transaminase (AST) and alanine transaminase (ALT), which are markers of hepatocellular damage, in SD rats. D-galactosamine-induced hepatocellular damage was also suppressed by ABG treatment. However, ABG does not affect the elevation of alkaline phosphatase (ALP), a marker of hepatobilliary damage, in rats treated with carbon tetrachloride or D-galactosamine. We also examined the effect of ABG on high-fat diet (HFD)-induced fatty liver and subsequent liver damage. ABG had no significant effect on body weight increase and plasma lipid profile in HFD-fed mice. However, HFD-induced increase in AST and ALT, but not ALP, was significantly suppressed by ABG treatment. These results demonstrate that ABG has hepatoprotective effects and suggest that ABG supplementation might be a good adjuvant therapy for the management of liver injury.

주제어

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가설 설정

Based on these reports, we hypothesized that ABG might also have protective effects against liver injuries induced by various insults. In the present study, we examined the effects of ABG on carbon tetrachloride (CCl₄)- or D-galac-tosamine- induced liver damage.

제안 방법

C57BL/6 mice were used for HFD-induced liver steatosis and injury model. The control mice were fed with a control diet (CD, D12450B, Research Diets, Inc., New Brunswick, NJ, USA) and the other mice were fed with a high-fat diet (HFD, D12492, Research Diets, Inc.) for 4 weeks before sample treatment. Vehicle or indicated concentrations of ABG (100, 200 or 400 mg/kg) were give five days a week for 4 weeks.
) for 4 weeks before sample treatment. Vehicle or indicated concentrations of ABG (100, 200 or 400 mg/kg) were give five days a week for 4 weeks. The body weight was recorded once a week.

대상 데이터

Louis, MO, USA) unless otherwise noted. Seven weeks old male SD (Sprague Dawley) rats and C57BL/6 mice were purchased from Koatech (Pyungtaek, Gyeonggi, Korea) and Biomedical Mouse Resource Center in KRIBB (Cheongwon, Chungbuk, Korea), respectively, and cared for as described previously (18). Animals were allowed to acclimate to the local environment for at least 1 week before use.

데이터처리

Results are expressed as mean ± S.D. Two-way ANOVA and Bonferroni’s multiple comparison test were used for body weight and One-way ANOVA and Dunnett’s t-test were used for another results for statistical analysis using GraphPad Prism (GraphPad Software; La Jolla, CA, USA).
Statistical significance was analyzed by one-way ANOVA and Dunnett’s t-test (* p < 0.05 vs. vehicle).
Statistical significant was analyzed by one-way ANOVA and Dunnett’s t-test (* p < 0.05 vs. vehicle).
Statistical significant was analyzed by one-way ANOVA and Dunnett’s t-test (* p < 0.05).

성능/효과

Our unpublished experiments, in which ABG was treated from the start of HFD feeding for 4 weeks, also support this notion by showing that HFD-mediated changes in plasma cholesterols were not affected by ABG treatment although the increases in body weight, body fat and plasma TG were significantly suppressed in HFD-fed mice. However, our results showed that the hepatoprotective effect of ABG is in consistent with the results of AGE and yeast-fermented black garlic extract, suggesting that the hepatoprotective potential against obesity- related hepatic steatosis are universal characteristics of all these garlic preparations.
The level of AST and ALT, which are known as a marker of hepatocellular damage, was also elevated significantly by CCl₄ treatment, and this was dose-dependently suppressed by ABG treatment (Table 1). In addition, the hepatobiliary damage, which is manifested by plasma ALP level, was increased significantly, and concentration-dependent, but statistically insignificant, inhibition of CCl₄-induced increase in ALP by ABG was observed (Table 1). Plasma ALB level was not changed in all groups examined in this experiment (Table 1).
We also assessed the hepatoprotective effect of ABG in HFD-induced obesity model. Our results demonstrated that ABG had no effect on HFD-induced body weight gain and plasma lipid profile, although plasma TG was slightly decreased by ABG treatment. However, HFD-induced increase in body fat was significantly suppressed by ABG treatment.

참고문헌 (32)

1 Amagase H. Milner J.A. Impact of various sources of garlic and their constituents on 7,12-dimethylbenz[a]anthracene binding to mammary cell DNA. Carcinogenesis (1993) 14 1627 1631 10.1093/carcin/14.8.1627 8353846

상세보기
2 Block E. The chemistry of garlic and onions. Sci. Am. (1985) 252 114 119 10.1038/scientificamerican0385-114 3975593

상세보기
3 Isensee H. Rietz B. Jacob R. Cardioprotective actions of garlic (Allium sativum). Arzneimittelforschung (1993) 43 94 98 8457243
4 Hoshino T. Kashimoto N. Kasuga S. Effects of garlic preparations on the gastrointestinal mucosa. J. Nutr. (2001) 131 1109S 1113S 11238827
5 Oboh G. Prevention of garlic-induced hemolytic anemia using some tropical green leafy vegetables. J. Med. Food (2004) 7 498 501 10.1089/jmf.2004.7.498 15671698

상세보기
6 McRae M.P. A review of studies of garlic (Allium sativum on serum lipids and blood pressure before and after 1994: does the amount of allicin released from garlic powder tablets play a role? J. Chiropr. Med. (2005) 4 182 190 10.1016/S0899-3467(07)60149-9 19674660

상세보기
7 Ishikawa H. Saeki T. Otani T. Suzuki T. Shimozuma K. Nishino H. Fukuda S. Morimoto K. Aged garlic extract prevents a decline of NK cell number and activity in patients with advanced cancer. J. Nutr. (2006) 136 816S 820S 16484572
8 Borek C. Antioxidant health effects of aged garlic extract. J. Nutr. (2001) 131 1010S 1015S 11238807
9 Uda N. Kashimoto N. Sumioka I. Kyo E. Sumi S. Fukushima S. Aged garlic extract inhibits development of putative preneoplastic lesions in rat hepatocarcinogenesis. J. Nutr. (2006) 136 855S 860S 16484580
10 Lau B.H. Yamasaki T. Gridley D.S. Garlic compounds modulate macrophage and T-lymphocyte functions. Mol. Biother. (1991) 3 103 107 10.1007/BF02172082 1910619

상세보기
11 Lee Y.M. Gweon O.C. Seo Y.J. Im J. Kang M.J. Kim M.J. Kim J.I. Antioxidant effect of garlic and aged black garlic in animal model of type 2 diabetes mellitus. Nutr. Res. Pract. (2009) 3 156 161 10.4162/nrp.2009.3.2.156 20016716

원문보기 상세보기
12 Kim M.H. Kim M.J. Lee J.H. Han J.I. Kim J.H. Sok D.E. Kim M.R. Hepatoprotective effect of aged black garlic on chronic alcohol-induced liver injury in rats. J. Med. Food (2011) 14 732 738 10.1089/jmf.2010.1454 21663494

원문보기 상세보기
13 Jang E.K. Seo J.H. Lee S.P. Physiological activity and antioxidative effects of aged black garlic (Allium sativum L.) extract. Korean J. Food Sci. Technol. (2008) 40 443 448
14 Kang M.J. Lee S.J. Shin J.H. Kang S.K. Kim J.G. Sung N.J. Effect of garlic with different procesing on lipid metabolism in 1% cholesterol fed rats. J. Korean Soc. Food Sci. Nutr. (2008) 37 162 169 10.3746/jkfn.2008.37.2.162

원문보기 상세보기
15 Seo Y.J. Gweon O.C. Lee Y.M. Kang M.J. Kim J.I. Effect of garlic and aged black garlic on hyperglycemis and dyslipidemia in animal model of type 2 diabetes mellitus. J. Food Sci. Nutr. (2009) 14 1 7 10.3746/jfn.2009.14.1.001

원문보기 상세보기
16 Jung Y.M. Lee S.H. Lee D.S. You M.J. Chung I.K. Cheon W.H. Kwon Y.S. Lee Y.J. Ku S.K. Fermented garlic protects diabetic obese mice when fed a highfat diet by antioxidant effects. Nutr. Res. (2011) 31 387 396 10.1016/j.nutres.2011.04.005 21636017

상세보기
17 Wang X. Jiao F. Wang Q.W. Wang J. Yang K. Hu R.R. Liu H.C. Wang H.Y. Wang Y.S. Aged black garlic extract induces inhibition of gastric cancer cell growth in vitro and in vivo. Mol. Med. Rep. (2012) 5 66 72 21922142
18 Kang J.S. Lee W.K. Lee C.W. Yoon W.K. Kim N. Park S.K. Lee H.S. Park H.K. Han S.B. Yun J. Lee K. Lee K.H. Park S.K. Kim H.M. Improvement of highfat diet-induced obesity by a mixture of red grape extract, soy isoflavone and L-carnitine: implications in cardiovascular and non-alcoholic fatty liver diseases. Food Chem. Toxicol. (2011) 49 2453 2458 10.1016/j.fct.2011.06.071 21745528

상세보기
19 Foretz M. Guichard C. Ferre P. Foufelle F. Sterol regulatory element binding protein-1c is a major mediator of insulin action on the hepatic expression of glucokinase and lipogenesis-related genes. Proc. Natl. Acad. Sci. U. S. A. (1999) 96 12737 12742 10.1073/pnas.96.22.12737 10535992

상세보기
20 Uemitsu N. Nishimura C. Nakayoshi H. Evaluation of liver weight changes following repeated administration of carbon tetrachloride in rats and body-liver weight relationship. Toxicology (1986) 40 181 190 10.1016/0300-483X(86)90077-6 3726893

상세보기
21 Domitroviæ R. Jakovac H. Tomac J. Sain I. Liver fibrosis in mice induced by carbon tetrachloride and its reversion by luteolin. Toxicol. Appl. Pharmacol. (2009) 241 311 321 10.1016/j.taap.2009.09.001 19747501

상세보기
22 Fujii T. Fuchs B.C. Yamada S. Lauwers G.Y. Kulu Y. Goodwin J.M. Lanuti M. Tanabe K.K. Mouse model of carbon tetrachloride induced liver fibrosis: Histopathological changes and expression of CD133 and epidermal growth factor. BMC Gastroenterol. (2010) 10 79 10.1186/1471-230X-10-79 20618941

상세보기
23 Qin Y. Tian Y.P. Protective effects of total glucosides of paeony and the underlying mechanisms in carbon tetrachloride- induced experimental liver injury. Arch. Med. Sci. (2011) 7 604 612 10.5114/aoms.2011.24129 22291795

상세보기
24 Boll M. Weber L.W. Becker E. Stampfl A. Mechanism of carbon tetrachloride-induced hepatotoxicity. Hepatocellular damage by reactive carbon tetrachloride metabolites. Z. Naturforsch. C (2001) 56 649 659 11531102
25 Nagi M.N. Alam K. Badary O.A. al-Shabanah O.A. al-Sawaf H.A. al-Bekairi A.M. Thymoquinone protects against carbon tetrachloride hepatotoxicity in mice via an antioxidant mechanism. Biochem. Mol. Biol. Int. (1999) 47 153 159 10092955

상세보기
26 Ajith T.A. Janardhanan K.K. Antioxidant and antihepatotoxic activities of Phellinus rimosus (Berk) Pilat. J. Ethnopharmacol. (2002) 81 387 391 10.1016/S0378-8741(02)00042-9 12127241

상세보기
27 Kuriakose G.C. Kurup M.G. Antioxidant and antihepatotoxic effect of Spirulina laxissima against carbon tetrachloride induced hepatotoxicity in rats. Food Funct. (2011) 2 190 196 10.1039/c0fo00163e 21779578

상세보기
28 Hassan M.H. Edfawy M. Mansour A. Hamed A.A. Antioxidant and antiapoptotic effects of capsaicin against carbon tetrachloride-induced hepatotoxicity in rats. Toxicol. Ind. Health (2012) 28 428 438 10.1177/0748233711413801 21859771

상세보기
29 Decker K. Keppler D. Galactosamine induced liver injury. Prog. Liver Dis. (1972) 4 183 199 4631501

상세보기
30 Shanmugarajan T.S. Sivaraman D. Somasundaram I. Arunsundar M. Krishnakumar E. Balaji R. Ravichandiran V. Influence of alpha lipoic acid on antioxidant status in D-galactosamine-induced hepatic injury. Toxicol. Ind. Health (2008) 24 635 642 10.1177/0748233708101215 19141568

상세보기
31 Yoo Y.M. Nam J.H. Kim M.Y. Choi J. Park H.J. Pectolinarin and pectolinarigenin of Cirsium setidens prevent the hepatic injury in rats caused by D-galactosamine via an antioxidant mechanism. Biol. Pharm. Bull. (2008) 31 760 764 10.1248/bpb.31.760 18379079

상세보기
32 Seo D.Y. Lee S. Figueroa A. Kwak Y.S. Kim N. Rhee B.D. Ko K.S. Bang H.S. Baek Y.H. Han J. Aged garlic extract enhances exercise-mediated improvement of metabolic parameters in high fat diet-induced obese rats. Nutr. Res. Pract. (2012) 6 513 519 10.4162/nrp.2012.6.6.513 23346301

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