선택한 단어 수는 입니다.
최소 단어 이상 선택하여야 합니다.
최소 단어 이상 선택하여야 합니다.
선택한 단어 수는 30입니다.
최대 10 단어까지만 선택 가능합니다.
최대 10 단어까지만 선택 가능합니다.
제안 방법
- Column chromatography was carried out using Kieselgel 60 (Merck, NJ, USA) and C-18 (Yamazen, Ultra Pack ODS-S-50B, Japan). 1H- and 13C-NMR analyses were performed with a Brucker Avance Digital 400 NMR spectrometer (Karlsruhe, Germany) at 400 and 100 MHz, respectively. Chemical shifts were given in δ (ppm) from tetramethylsilane.
- Louis, MO, USA). Column chromatography was carried out using Kieselgel 60 (Merck, NJ, USA) and C-18 (Yamazen, Ultra Pack ODS-S-50B, Japan). 1H- and 13C-NMR analyses were performed with a Brucker Avance Digital 400 NMR spectrometer (Karlsruhe, Germany) at 400 and 100 MHz, respectively.
- HPLC analysis was performed using a HPLC system consisting of Dionex Ultimate 3000 binary pump, automated sample injector, thermostatted column compartment, and Agilent 380-ELSD with Alltech Prosphere 300 C-4 column (250 × 4.6 mm id, 5 μm).
- 16 However, there are no reports on the detection and analysis of euscaphic acid in Chaenomelis Fructus. In this study, the simultaneous determination of ursolic acid and euscaphic acid as marker compounds to ensure the quality of Chaenomelis Fructus was performed by HPLC-ELSD. The method was validated and applied to assess the quality of Chaenomelis Fructus cultivated in different sites.
- The precision and accuracy were determined by analyzing each standard sample at a concentration of 10 μg/mL. Intra-day precision was determined by repeating the analysis of each standard sample five times in a single day, and inter-day precision and accuracy were determined by repeating the analysis on three consecutive days. LOD and LOQ were defined as the minimum concentration at the signal-to-noise ratio (S/N) = 3 and 10, respectively.
- The validated HPLC method was used to analyze two marker compounds, ursolic acid and euscaphic acid, in ten samples collected from different locations in Korea (Table 2). In all ten samples, the contents of ursolic acid were higher than those of euscaphic acid.
대상 데이터
- Dried Chaenomelis Fructus samples from ten different habitats were obtained from herbal medicine markets in Daegu and Seoul, Korea. A voucher specimen was deposited at the Laboratory of Natural Products Medicine, College of Pharmacy, Kyungpook National University. Ethanol, dichloromethane, ethyl acetate, n-butanol, and methanol were obtained from Duksan Chemical (Anseong, Korea).
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