[논문]EMS-induced Mutagenesis for C18 Unsaturated Fatty Acids in Rapeseed (Brassica napus L.)

Lee, Yong-Hwa; Kim, Kwang-Soo; Jang, Young-Seok; Choi, In-Hu

doi:10.7740/kjcs.2014.59.2.128

EMS-induced Mutagenesis for C18 Unsaturated Fatty Acids in Rapeseed (Brassica napus L.) 원문보기

Korean journal of crop science = 韓國作物學會誌, v.59 no.2, 2014년, pp.128 - 133

Lee, Yong-Hwa (Bioenergy Crop Research Center, National Institute of Crop Science, Rural Development Administration) , Kim, Kwang-Soo (Bioenergy Crop Research Center, National Institute of Crop Science, Rural Development Administration) , Jang, Young-Seok (Bioenergy Crop Research Center, National Institute of Crop Science, Rural Development Administration) , Choi, In-Hu (Bioenergy Crop Research Center, National Institute of Crop Science, Rural Development Administration)

Abstract ▼ AI-Helper

Rapeseed (Brassica napus L.) oil with high oleic acid content is of great interest for both food and non-food uses. The 'Tamla' variety, characterized by oleic acid content of approximately 69%, was treated with 1% ethyl methane sulfonate (EMS) to induce mutations in the fatty acid biosynthesis pathway. $M_1$ plants were selfed and subsequent generations ($M_2$, $M_3$, and $M_4$ mutants) were analyzed to identify mutants having increased levels of oleic acid. $M_2$ mutants showed oleic acid content ranging from 13.5% to 76.9% with some mutants (TR-458 and TR-544) having up to 74.7% and 76.9% oleic acid, which was an increase of nearly 5% and 7%, respectively, compared to untreated cv 'Tamla'. We selected two $M_3$ mutants with >75% oleic acid content. One mutant (TR-458-2) had increased oleic acid (75.9%) and decreased linoleic acid (12.5%) and linolenic acid (4.4%) contents. The other (TR-544-1) showed increased oleic acid content (75.7%) and decreased linoleic acid (13.5%) and linolenic acid (3.3%) contents. The accumulation or reduction of oleic acid content in the selected $M_4$ mutants was also accompanied by a simultaneous decrease or increase in linoleic and linolenic acid contents. The high-oleic lines could be utilized further in breeding programs for improvement of rapeseed oil quality.

주제어

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문제 정의

Our objective was to identify changes in fatty acid composition among Brassica napus L. ‘Tamla’ mutants after EMS treatment.
The objective of this study was to develop rapeseed mutants with beneficially altered fatty acid composition from B. napus ‘Tamla’ variety by EMS mutagen treatment.

제안 방법

To investigate the stability of oleic acid level in the TR-458 and TR-544 mutants, the M₃ seeds of the mutants were grown to plants and oleic acid content in M₄ seeds of the single-plant progenies was analyzed (Fig. 2). M₄ seeds of TR-458-2 and TR-544-1 had ≥ 75% oleic acid content and a reduction in linoleic and linolenic acids equivalent to the increase in oleic acid (Fig.

대상 데이터

The seeds were then rinsed under water for >4 h and dried on filter paper at room temperature. The seeds were sown into soil in a greenhouse at the Bioenergy Crop Research Center, National Institute of Crop Science, Muan, South Korea in December 2009.
The seed oil content of selected mutants was determined by extraction with petroleum ether using a Soxtec™ 2050 Auto Fat Extraction System (Foss Tecator, Sweden) and calculated by the percentage of oil-free dry solids. Three replicates per sample were used.

참고문헌 (17)

Auld, D. L., M. K. Heikkinen, D. A. Erickson, J. L. Sernyk, and J. E. Romero. 1992. Rapeseed mutants with reduced levels of polyunsaturated fatty acids and increased levels of oleic acid. Crop Science. 32(3) : 657-662.

상세보기
Buhsenschuz-Nothdurft, A., A. Schuster, and W. Friedt. 1998. Breeding for modified fatty acid composition via experimental mutagenesis in Camelina sativa (L.) Crtz. Industrial Crops and Products. 7(2) : 291-295.

상세보기
Craig, B. M. 1961. Varietal and environmental effects on rapeseed. 3. Fatty acid composition of 1958 varietal tests. Canadian Journal of Plant Science. 41 : 204-210.

상세보기
Jacobs, M. 1969. Comparison de I'action mutagenique d'agents alkylants et des radiations gamina chez Arabidopsis thaliana. Radiation Botany. 9 : 251-268.

상세보기
James, D. W. and H. K. Dooner. 1990. Isolation of EMSinduced mutants in Arabidopsis altered in seed fatty acid composition. Theoretical and Applied Genetics. 80(2) : 241-245.
Jonsson, R. 1977. Erucic-acid heredity in rapeseed Brassica napus L. and Brassica campestris. Hereditas. 86(2) : 159-170.
Jang, Y. S., K. S. Kim, Y. H. Lee, H. J. Cho, and S. J. Suh. 2010. Review of property and utilization of oil crop for biodiesel. Journal of Plant Biotechnology. 37(1) : 25-46.

원문보기 상세보기
Lee, Y. H., K. S. Kim, Y. S. Jang, H. J. Cho, S. S. Nam, and S. J. Suh. 2010. Breeding of $F_1$ hybrid rapeseed and the cultivation for biodiesel production in Korea. The Journal of the Korean Society of International Agriculture. 22(4) : 341-345.
Patil, A., S. P. Taware, M. D. Oak, S. A. Tamhankar, and V. S. Rao. 2007. Improvement of oil quality in soybean [Glycine max (L.) Merrill] by mutation breeding. Journal of the American Oil Chemists' Society. 84(12) : 1117-1124

상세보기
Rahman, S. M., Y. Takagi, K. Kubota, K. Miyamoto, and T. Kawakita. 1994. High oleic acid mutant in soybean induced by x-ray irradiation. Bioscience, Biotechnology, and Biochemistry. 58(6) : 1070-1072.

상세보기
Rakow, G. and D. I. McGregor. 1973. Opportunities and problems in modification of levels of rapeseed C18 unsaturated fatty acids. Journal of the American Oil Chemists' Society. 50(10) : 400-403.

상세보기
Robbelen, G. and K. Kraling. 1993. Rapeseed oils high in single fatty acid contents for oleochemical uses. Industrial Crops and Products. 1 : 303-309.
Savant, K. D. and V. S. Kothekar. 2011. Induction of variability in fatty acid profile in sesame (Sesamum indicum L.). Journal of Phytology. 3(12) : 1-3.
Sheikh, F.A., B. Lone, S. Najeeb, A. B. Shikari, G. A. Parray, R. R. Rather, and R. S. Khudwani. 2009. Induced mutagenesis for seed quality traits in Ethiopian mustard (Brassica carinata A. Braun). Journal of Agricultural and Biological Science. 4(2) : 42-46.
Spasibionek, S. 2006. New mutants of winter rapeseed (Brassica napus L.) with changed fatty acid composition. Plant Breeding. 125(3) : 259-267.

상세보기
Velasco, L., J. M. Fernandez-Martinez, and A. De Haro. 1997. Induced variability for C18 unsaturated fatty acids in Ethiopian mustard. Canadian Journal of Plant Science. 77(1) : 91-95.

상세보기
Zhao, J., Z. Dimov, H. C. Becker, W. Ecke, and C. Molers. 2008. Mapping QTL controlling fatty acid composition in a doubled haploid rapeseed population segregating for oil content. Molecular Breeding. 21(1) : 115-125.

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