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[국내논문] First Report of Potato Stem-End Rot Caused by Fusarium oxysporum in Korea 원문보기

Mycobiology, v.42 no.2, 2014년, pp.206 - 209  

Aktaruzzaman, Md. (Department of Plant Science, Gangneung-Wonju National University) ,  Xu, Sheng-Jun (Department of Plant Science, Gangneung-Wonju National University) ,  Kim, Joon-Young (Department of Plant Science, Gangneung-Wonju National University) ,  Woo, Jae-Hyoun (Department of Plant Science, Gangneung-Wonju National University) ,  Hahm, Young-Il (Daegwallyong Horticultural Cooperative) ,  Kim, Byung-Sup (Department of Plant Science, Gangneung-Wonju National University)

Abstract ▼ AI-Helper 아이콘AI-Helper

In this study, we identified the causative agent of stem-end rot in potatoes that were grown in Gangwon alpine areas of Korea in 2013. The disease symptoms included appearance of slightly sunken circular lesion with corky rot on the potato surface at the stem-end portion. The fungal species isolated...

주제어

#Fusarium oxysporum   #Pathogenicity   #Solanum tubersum   #Stem-end rot of potato  

AI 본문요약
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제안 방법

  • The extracted DNA was used for PCR sequencing of rDNA genes by using universal primers for internal transcribed spacer (ITS) 1 (5'-TCCGTAGGTGAACCTGCGG-3') and ITS 4 (5'-TCCTCCGCTTATTGATATGC-3') [12].

대상 데이터

  • oxysporum was performed by using the MEGA5 program with the neighbor-joining method [14]. Sequence data were deposited in GenBank (accession No. KJ162149).
  • The reaction was performed in Eppendorf Mastercycler Gradient (Eppendorf, Hamburg, Germany) under the following conditions: pre-denaturation at 94o C for 5 min, followed by 35 cycles of denaturation at 94℃ for 35 sec, annealing at 52℃ for 55 sec, and elongation at 72℃ for 1 min and then final extension at 72℃ for 10 min [13]. The obtained nucleotide sequences were searched through BLASTN at the GenBank database (http:// www.ncbi.nlm.nih.gov/BLAST/). Phylogenetic analysis of F.

이론/모형

  • gov/BLAST/). Phylogenetic analysis of F. oxysporum was performed by using the MEGA5 program with the neighbor-joining method [14]. Sequence data were deposited in GenBank (accession No.
  • The ITS sequence was compared to the GenBank database sequences by using the NCBI BLAST search tool. The sequences identified based on rRNA-ITS alignment were 100% similar to those of several F.
  • DNA extraction, PCR, and sequence analysis. The pathogen was isolated from the disease lesion of the potatoes by using the surface sterilization method. Mycelia were obtained from a 7-day-old culture on PDA.
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참고문헌 (15)

  1. 1 Muthoni J Nyamongo DO A review of constraints to ware Irish potatoes production in Kenya J Hortic For 2009 1 98 102 

  2. 2 Information and Statistics Division of Korean Ministry for Food, Agriculture, Forestry and Fisheries Food, agriculture, forestry and fisheries statistical year book 2012 Gwacheon Korean Ministry for Food, Agriculture, Forestry and Fisheries 2012 100 101 

  3. 3 Summerell BA Laurence MH Liew EC Leslie JF Biogeography and phylogeography of Fusarium: a review Fungal Divers 2010 44 3 13 

  4. 4 Michielse CB Rep M Pathogen profile update: Fusarium oxysporum Mol Plant Pathol 2009 10 311 324 19400835 

    상세보기

  5. 5 Enya J Togawa M Takeuchi T Yoshida S Tsushima S Arie T Sakai T Biological and phylogenetic characterization of Fusarium oxysporum complex, which causes yellows on Brassica spp, and proposal of F. oxysporum f. sp. rapae , a novel forma specialis pathogenic on B. rapa in Japan Phytopathology 2008 98 475 483 18944198 

  6. 6 Garcia Bayona L Grajales A Cardenas ME Sierra R Cepero de Garcia MC Bernal A Jimenez P Restrepo S First report of Fusarium oxysporum causing potato dry rot in Solanum tuberosum in Colombia New Dis Rep 2011 24 14 

  7. 7 Theron DJ Holz G Fusarium species associated with dry and stem-end rot of potatoes in South Africa Phytophylactica 1989 21 175 181 

  8. 8 Brewer PJ Potato stem-end rot caused by Fusarium spp S Afr J Agric Sci 1962 5 475 481 

  9. 9 Pont W Studies on tuber stem end rot and stem rot of potato in north Queensland caused by Fusarium solani and Fusarium oxysporum Queensland J Agric Anim Sci 1976 33 129 144 

  10. 10 MacMillan HG Meckstroth GA The critical temperature for infection of the potato seed piece by Fusarium oxysporum J Agric Res 1925 32 917 921 

  11. 11 Möller EM Bahnweg G Sandermann H Geiger HH A simple and efficient protocol for isolation of high molecular weight DNA from filamentous fungi, fruit bodies, and infected plant tissues Nucleic Acids Res 1992 20 6115 6116 1461751 

    상세보기

  12. 12 White TJ Bruns T Lee S Taylor JW Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics Innis MA Gelfand DH Sninsky JJ White TJ PCR protocols: a guide to methods and applications San Diego Academic Press 1990 315 322 

  13. 13 Nishizawa T Zhaorigetu Komatsuzaki M Sato Y Kaneko N Ohta H Molecular characterization of fungal communities in non-tilled, cover-cropped upland rice field soils Microbes Environ 2010 25 204 210 21597240 

  14. 14 Tamura K Peterson D Peterson N Stecher G Nei M Kumar S MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods Mol Biol Evol 2011 28 2731 2739 21546353 

    상세보기

  15. 15 Booth C Fusarium oxysporum . CMI descriptions of pathogenic fungi and bacteria Kew, Surrey Commonwealth Mycological Institute 1970 

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