무릎 골관절증을 보이는 개에서 자가지방유래 중배엽성 줄기세포 치료 다섯 증례 Clinical Application of Autologous Adipose Tissue Derived Mesenchymal Stem Cells in Five Dogs with Stifle Joint Osteoarthrosis원문보기
후지 파행 증상을 보이는 다섯 마리 개가 내원하였다. 무릎 관절 신체 검사에서 통증, 파행, 슬개골 탈구, 또는 인대 손상을 확인 하였다. 일반 방사선 사진에서 골증식체와 연골하 병변을 확인 하였고 골관절증 점수가 높은 것을 확인하였다. 수술적 교정과 자가지방 유래 줄기세포 치료의 혼합 방법 또는 자가지방 유래 줄기세포의 경피 주입단독 방법을 보호자의 동의 하에 실시 하였다. $1{\times}10^6$ 개의 줄기세포를 PBS와 함께 주입 하였다. 수술 후 확인은 수의사의 신체검사를 통해 12개월까지 실시 하였다. 주입 후 6개월 또는 12 개월에 파행, 통증, 골관절증 점수가 개선되었음을 확인 하였고 일반 방사선 사진에서도 골증식체와 연골하 병변이 줄어든 것을 확인 할 수 있었다. 본 증례들를 통해 자가지방 유래 줄기 세포 치료가 골관절증을 보이는 개에서 효과적인 방법임을 확인 할 수 있었다.
후지 파행 증상을 보이는 다섯 마리 개가 내원하였다. 무릎 관절 신체 검사에서 통증, 파행, 슬개골 탈구, 또는 인대 손상을 확인 하였다. 일반 방사선 사진에서 골증식체와 연골하 병변을 확인 하였고 골관절증 점수가 높은 것을 확인하였다. 수술적 교정과 자가지방 유래 줄기세포 치료의 혼합 방법 또는 자가지방 유래 줄기세포의 경피 주입단독 방법을 보호자의 동의 하에 실시 하였다. $1{\times}10^6$ 개의 줄기세포를 PBS와 함께 주입 하였다. 수술 후 확인은 수의사의 신체검사를 통해 12개월까지 실시 하였다. 주입 후 6개월 또는 12 개월에 파행, 통증, 골관절증 점수가 개선되었음을 확인 하였고 일반 방사선 사진에서도 골증식체와 연골하 병변이 줄어든 것을 확인 할 수 있었다. 본 증례들를 통해 자가지방 유래 줄기 세포 치료가 골관절증을 보이는 개에서 효과적인 방법임을 확인 할 수 있었다.
Five dogs presented with a history of pelvic limb lameness. On physical examination of the stifle joints, five dogs had pain, lameness, patellar luxation, or ligamentous instability. Craniocaudal and mediolateral radiographic projections revealed osteophytes or subchondral cystic lesions on the stif...
Five dogs presented with a history of pelvic limb lameness. On physical examination of the stifle joints, five dogs had pain, lameness, patellar luxation, or ligamentous instability. Craniocaudal and mediolateral radiographic projections revealed osteophytes or subchondral cystic lesions on the stifle joints. Based on a previously described Osteoarthrosis (OA) scoring technique, five dogs showed high OA scores. Combination of surgery and implantation of autologous adipose tissue derived mesenchymal stem cells (aAT-MSCs) or percutaneous injection of aAT-MSCs was determined with informed consent. $1{\times}10^6$ aAT-MSCs suspended in PBS was injected in the stifle joints. The follow-ups were completed 12 months after surgery. The follow-up information was based on physical examination by veterinarians. The lameness, pain on manipulation, and OA scores improved six or 12 months after implantation of aAT-MSCs. There was a radiographic evidence of decreased osteophytes and subchondral cystic lesions. These results suggest that implantation of aAT-MSCs can be considered an option for management of cases of OA in the stifle joints.
Five dogs presented with a history of pelvic limb lameness. On physical examination of the stifle joints, five dogs had pain, lameness, patellar luxation, or ligamentous instability. Craniocaudal and mediolateral radiographic projections revealed osteophytes or subchondral cystic lesions on the stifle joints. Based on a previously described Osteoarthrosis (OA) scoring technique, five dogs showed high OA scores. Combination of surgery and implantation of autologous adipose tissue derived mesenchymal stem cells (aAT-MSCs) or percutaneous injection of aAT-MSCs was determined with informed consent. $1{\times}10^6$ aAT-MSCs suspended in PBS was injected in the stifle joints. The follow-ups were completed 12 months after surgery. The follow-up information was based on physical examination by veterinarians. The lameness, pain on manipulation, and OA scores improved six or 12 months after implantation of aAT-MSCs. There was a radiographic evidence of decreased osteophytes and subchondral cystic lesions. These results suggest that implantation of aAT-MSCs can be considered an option for management of cases of OA in the stifle joints.
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제안 방법
Craniocaudal and mediolateral radiographic projections revealed osteophytes or subchondral cystic lesions on the right and left stifle joints (Fig 1). Based on a previously described OA scoring technique (13), an OA score for each stifle was determined by evaluating 32 specific radiographic features of OA. Osteoarthrosis scores for the right and left stifle joints were 32 and 29 respectively.
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