[논문]추간판 탈출증이 발생한 개에서 동종의 지방유래 줄기세포의 임상적 적용

김영기; 이승용; 박세진; 이스캇; 김진현; 이희천; 장홍희; 이효종; 연성찬

추간판 탈출증이 발생한 개에서 동종의 지방유래 줄기세포의 임상적 적용
Clinical Application of Mesenchymal Stem Cells in a Dog with Intervertebral Disc Disease 원문보기

Journal of veterinary clinics = 한국임상수의학회지, v.28 no.1 = no.78, 2011년, pp.122 - 127

초록
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후지마비와 심부통각의 소실을 보이는 5 년령의 수컷 Cocker Spaniel이 내원하였다. 신체검사, 신경검사, 방사선검사와 컴퓨터 단층촬영결과 심부통각의 소실을 동반한 요추 2번과 3번 사이의 디스크 탈출증으로 진단하였다. 편측 추궁 절제술을 시행하여 감압술과 탈출된 디스크 물질을 제거하였으며 $1{\times}10^6$ 개의 동종 지방유래 줄기세포를 $50{\mu}l$ 생리식염수에 희석하여 손상된 척수에 직접 주입하였다. 수술 10주 후, 양쪽 후지 모두에서 심부통각과 운동기능이 완전히 회복되었으며 세포이식과 관련된 부작용은 현재까지 발견되지 않았다. 본 증례를 통해 심각한 정도의 디스크질환을 가진 환축에서 감압술과 병행된 줄기세포의 이식방법은 좀더 나은 예후를 기대하기 위한 치료방법들 중 하나로 고려해볼 수 있을 것으로 생각된다.

Abstract ▼ AI-Helper

A 5-year old, intact male, Cocker spaniel dog was referred with paraplegia and loss of deep pain perception. Physical, neurological examinations, radiography, and computed tomography were evaluated. Based on the clinical examinations, the dog was diagnosed with severe disc herniation ($L_2$ to $L_3$ intervertebral disc space). On the next day of presentation (6 days after loss of deep pain perception), hemilaminectomy was performed. After decompression of spinal cord and removal of herniated disc materials, $1{\times}10^6$ canine allogenic adipose tissue-derived mesenchymal stem cells (MSCs) diluted by $50{\mu}l$ saline were directly applied to the injured site of the spinal cord. Ten weeks of follow-up after surgery, full recovery of deep pain perception and motor function were evaluated in both hind limbs. Based on the result, we suggest that the transplantation of allogenic adipose tissue-derived MSCs to dogs with spinal cord injuries could be a considerable method to expect better clinical outcomes in veterinary practice.

주제어

AI 본문요약
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문제 정의

However, the spinal cord injuries involved in these studies were occurred experimentally in dog model. Thus, this current study was aimed to report the beneficial effects of the MSCs transplantation after surgical decompression in a dog with SCI occurred spontaneously in actual veterinary practice.

제안 방법

mildly, it was not enough to characterize the lesion. The helical computed tomography (CT) scan of the thoracolumbar portion of the vertebral column was performed. On CT scans, a large mineralized intervertebral disc was presented within the right ventral side of the L₂ - L₃ vertebral canal, displacing the spinal cord dorsally and to the left.

대상 데이터

Five years old, 13 kg intact male Cocker spaniel was presented at the Gyeongsang national university animal medical center owing to 6 days history of non-ambulation. Routine blood analyses and serum biochemical analyses were performed, but the results were unremarkable.

이론/모형

Daily behavioral analysis was performed to measure the degree of functional recovery of the pelvic limbs during 20 days of hospitalization. Functional recovery was scored according to the Olby scoring system (21) (Fig 4). The procedures of behavioral analysis were standardized.

성능/효과

The prognosis is excellent for dogs with grade I, II, and III deficits, especially following surgical treatments and also, dogs with grade III and IV deficits have a better outcome after decompression (28). However, the prognosis for dogs with grade V deficits is less optimistic and its published recovery rates following surgical decompression was ranged from 0 to 76 percent (27). Moreover, based on the previous study (26), when deep pain perception (DPP) has been absent for more than 48 h, surgical approach is seldom recommended.
In conclusion, the combination of surgical decompression and allogenic adipose tissue-derived MSCs transplantation yield beneficial effects on recovery of neurologic function in a dog with spontaneously occurred spinal cord injury. However, since the present study was considering the practical aspect of clinic, laboratory and molecular works including histopathology and postmortem examination could not be involved.

후속연구

However, the method used in the present study is not the best option for transplantation stem cells into the injured spinal cord because it should be accompanied by surgical intervention including hemilaminectomy. Thus, in order to find the most appropriate and effective method to apply in clinical practice, further studies investigating effectiveness of the various injection methods should be performed.

참고문헌 (32)

Barnett SC, Riddell JS. Olfactory ensheathing cell transplantation as a strategy for spinal cord repair - what can it achieve?. Nat Clin Pract Neurol 2007; 3: 152-161.

상세보기
Brown NO, Helphrey ML, Prata RG. Thoracolumbar disk disease in the dog: A retrospective analysis of 187 cases. J Am Anim Hosp Assoc 1977; 13: 665-672.
Cova L, Ratti A, Volta M, Fogh I, Cardin V, Corbo M, Silani V. Stem cell therapy for neurodegenerative diseases: The issue of transdifferentiation. Stem Cells Dev 2004; 13: 121-131.
Cowan CM, Shi YY, Aalami OO, Chou YF, Mari C, Thomas R, Quarto N, Contag CH, Wu B, Longaker MT. Adiposederived adult stromal cells heal critical-size mouse calvarial defects. Nat Biotechnol 2004; 22: 560-567.

상세보기
Dhupa S, Glickman NW, Waters DJ. Functional outcome in dogs after surgical treatment of caudal lumbar intervertebral disk herniation. J Am Anim Hosp Assoc 1999; 35: 323-331.

상세보기
Erices A, Conget P, Minguell JJ. Mesenchymal progenitor cells in human umbilical cord blood. Br J Haematol 2000; 109: 235-242.

상세보기
Ferreira AJA, Correia JHD, Jaggy A. Thoracolumbar disc disease in 71 paraplegic dogs: Influence of rate of onset and duration of clinical signs on treatment results. J Small Anim Pract 2002; 43: 158-163.

상세보기
James PT, David JW. Intervertebral disc disease. In: Textbook of Small Animal Surgery, 3rd ed. Philadelphia: Saunders. 2002: 1193-1209.
Gimble JM, Guilak F. Adipose-derived adult stem cells: Isolation, characterization, and differentiation potential. Cytotherapy 2003; 5: 362-369.

상세보기
Hiyama A, Mochida J, Iwashina T, Omi H, Watanabe T, Serigano K, Tamura F, Sakai D. Transplantation of mesenchymal stem cells in a canine disc degeneration model. J Orthop Res 2008; 26: 589-600.

상세보기
Hui L, Feng SQ, Chen JT, Han MY, Wang CY, Yu TQ. Repair of spinal cord injury using schwann cells in rats: Feasibility and superiority of intravenous transplantation. J Clin Rehab Tissue Eng Res 2010; 14: 3305-3309.
Joo YO, Mee KK, Mi SS, Hyun JL, Jung HK, Won RW, Jin HL. The anti-inflammatory and anti-angiogenic role of mesenchymal stem cells in corneal wound healing following chemical injury. Stem Cells 2008; 26: 1047-1055.

상세보기
Jung DI, Ha J, Kang BT, Kim JW, Quan FS, Lee JH, Woo EJ, Park HM. A comparison of autologous and allogenic bone marrow-derived mesenchymal stem cell transplantation in canine spinal cord injury. J Neurol Sci 2009; 285: 67-77.

상세보기
Kang SK, Lee DH, Bae YC, Kim HK, Baik SY, Jung JS. Improvement of neurological deficits by intracerebral transplantation of human adipose tissue-derived stromal cells after cerebral ischemia in rats. Exp Neurol 2003; 183: 355-366.

상세보기
Kang SK, Shin MJ, Jung JS, Kim YG, Kim CH. Autologous adipose tissue-derived stromal cells for treatment of spinal cord injury. Stem Cells Dev 2006; 15: 583-594.
Lepore AC, Bakshi A, Swanger SA, Rao MS, Fischer I. Neural precursor cells can be delivered into the injured cervical spinal cord by intrathecal injection at the lumbar cord. Brain Res 2005; 1045: 206-216.

상세보기
Lim JH, Byeon YE, Ryu HH, Jeong YH, Lee YW, Wan HK, Kang KS, Kweon OK. Transplantation of canine umbilical cord blood-derived mesenchymal stem cells in experimentally induced spinal cord injured dogs. J Vet Sci 2007; 8: 275-282.

상세보기
Lu P, Jones LL, Snyder EY, Tuszynski MH. Neural stem cells constitutively secrete neurotrophic factors and promote extensive host axonal growth after spinal cord injury. Exp Neurol 2003; 181: 115-129.

상세보기
Nishio Y, Koda M, Kamada T, Someya Y, Yoshinaga K, Okada S, Harada H, Okawa A, Moriya H, Yamazaki M. The use of hemopoietic stem cells derived from human umbilical cord blood to promote restoration of spinal cord tissue and recovery of hindlimb function in adult rats. J Neurosurg Spine 2006; 5: 424-433.
Olby N, Levine J, Harris T, Munana K, Skeen T, Sharp N. Long-term functional outcome of dogs with severe injuries of the thoracolumbar spinal cord: 87 cases (1996-2001). J Am Vet Med Assoc 2003; 222: 762-769.

상세보기
Olby NJ, De Risio L, Munana KR, Wosar MA, Skeen TM, Sharp NJH, Keene BW. Development of a functional scoring system in dogs with acute spinal cord injuries. Am J Vet Res 2001; 62: 1624-1628.

상세보기
Osaka M, Honmou O, Murakami T, Nonaka T, Houkin K, Hamada H, Kocsis JD. Intravenous administration of mesenchymal stem cells derived from bone marrow after contusive spinal cord injury improves functional outcome. Brain Res 2010; 1343: 226-235.

상세보기
Prata RG. Neurosurgical treatment of thoracolumbar disks: The rationale and value of laminectomy with concomitant disk removal. J Am Anim Hosp Assoc 1981; 17: 17-26.
Ryu HH, Lim JH, Byeon YE, Park JR, Seo MS, Lee YW, Kim WH, Kang KS, Kweon OK. Functional recovery and neural differentiation after transplantation of allogenic adiposederived stem cells in a canine model of acute spinal cord injury. J Vet Sci 2009; 10: 273-284.

상세보기
Satake K, Lou J, Lenke LG. Migration of mesenchymal stem cells through cerebrospinal fluid into injured spinal cord tissue. Spine 2004; 29: 1971-1979.

상세보기
Scott HW. Hemilaminectomy for the treatment of thoracolumbar disc disease in the dog: A follow-up study of 40 cases. J Small Anim Pract 1997; 38: 488-494.

상세보기
Scott HW, Mckee WM. Laminectomy for 34 dogs with thoracolumbar intervertebral disc disease and loss of deep pain perception. J Small Anim Pract 1999; 40: 417-422.

상세보기
Sharp NJH, Wheeler SJ. Thoracolumbar disc disease. In: Small Animal Spinal Disorders: Diagnosis and Surgery 2nd ed. Philadelphia: Mosby. 2005: 121-160.
Wu S, Suzuki Y, Ejiri Y, Noda T, Bai H, Kitada M, Kataoka K, Ohta M, Chou H, Ide C. Bone marrow stromal cells enhance differentiation of cocultured neurosphere cells and promote regeneration of injured spinal cord. J Neurosci Res 2003; 72: 343-351.

상세보기
Zhao LR, Duan WM, Reyes M, Keene CD, Verfaillie CM, Low WC. Human bone marrow stem cells exhibit neural phenotypes and ameliorate neurological deficits after grafting into the ischemic brain of rats. Exp Neurol 2002; 174: 11-20.

상세보기
Zhao ZM, Li HJ, Liu HY, Lu SH, Yang RC, Zhang QJ, Han ZC. Intraspinal transplantation of CD34+ human umbilical cord blood cells after spinal cord hemisection injury improves functional recovery in adult rats. Cell Transplant 2004; 13: 113-122.
Zurita M, Vaquero J. Bone marrow stromal cells can achieve cure of chronic paraplegic rats: Functional and morphological outcome one year after transplantation. Neurosci Lett 2006; 402: 51-56.

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