맥동전자장이 고지혈증 흰쥐의 혈액 성분, 항산화 효소 및 활성 산소에 미치는 영향 The Effects of Pulsed Electromagnetic Fields on Blood components, Antioxidant enzymes and Reactive Oxygen in Hyperlipidemic Rats원문보기
본 연구는 맥동전자장이 고지혈증 관련 혈액 성분, 항산화 효소와 활성 산소에 어떠한 영향을 미치는 지를 관찰하기 위하여 실시하였다. 실험군은 정상식이군, 고지방식이군과 고지방식이-맥동전자장 적용군의 3군으로 나누었다. 맥동 전자장은 맥동 전자장 에너지 치료기를 이용하였다. 혈액 성분의 검사 항목은 혈당, 유리지방산, 중성지방 및 콜레스테롤, 인슐린이고, 항산화 효소와 활성 산소의 검사는 글루타치온, 글루타치온 환원효소, 크산틴, 과산화지질을 측정하였다. 그 결과 맥동전자장 적용은 혈액 구성성분과 GSH와 GRD를 고지장식이 수준에서 정상식이 수준으로 상승시켰으며, XO와 MDA는 고지방식이 수준에서 정상 식이 수준으로 낮추어 주는 개선효과를 나타내었다. 그러므로 맥동전자장의 적용은 고지혈증 흰쥐의 혈액성분의 변화, 항산화 효소의 증가, 활성 산화의 억제에 효과적인 치료 방법이라 할 수 있다.
본 연구는 맥동전자장이 고지혈증 관련 혈액 성분, 항산화 효소와 활성 산소에 어떠한 영향을 미치는 지를 관찰하기 위하여 실시하였다. 실험군은 정상식이군, 고지방식이군과 고지방식이-맥동전자장 적용군의 3군으로 나누었다. 맥동 전자장은 맥동 전자장 에너지 치료기를 이용하였다. 혈액 성분의 검사 항목은 혈당, 유리지방산, 중성지방 및 콜레스테롤, 인슐린이고, 항산화 효소와 활성 산소의 검사는 글루타치온, 글루타치온 환원효소, 크산틴, 과산화지질을 측정하였다. 그 결과 맥동전자장 적용은 혈액 구성성분과 GSH와 GRD를 고지장식이 수준에서 정상식이 수준으로 상승시켰으며, XO와 MDA는 고지방식이 수준에서 정상 식이 수준으로 낮추어 주는 개선효과를 나타내었다. 그러므로 맥동전자장의 적용은 고지혈증 흰쥐의 혈액성분의 변화, 항산화 효소의 증가, 활성 산화의 억제에 효과적인 치료 방법이라 할 수 있다.
The present study was aimed to investigate the effects of the application of pulsed electromagnetic fields on the blood components associated hyperlipidemia, antioxidant enzymes and reactive oxygen. The subjects were divided into three groups: General Diet, High fat Diet, High fat diet and pulsed el...
The present study was aimed to investigate the effects of the application of pulsed electromagnetic fields on the blood components associated hyperlipidemia, antioxidant enzymes and reactive oxygen. The subjects were divided into three groups: General Diet, High fat Diet, High fat diet and pulsed electromagnetic fields. Pulsed electromagnetic field was applied with pulsed electromagnetic energy therapy equipment. The Glucose, free fatty acids, triglycerides, cholesterol and insulin was used to measure hyperlipidemia-related blood components, and the GSH, GRD, XO and MDA was used to measure antioxidant enzymes and reactive oxygen. The applied pulsed electromagnetic field has improved the concentrations of blood components and increasing GSH and GRD, that were decreased due to a high-fat diet and by reducing the increased MDA and XO to a level of general diet group. Therefore, the applied pulsed electromagnetic field can be the effective treatment for changes in the blood composition of hyperlipidemic rats, enhancement of antioxidative activity and the inhibition of reactive oxygen.
The present study was aimed to investigate the effects of the application of pulsed electromagnetic fields on the blood components associated hyperlipidemia, antioxidant enzymes and reactive oxygen. The subjects were divided into three groups: General Diet, High fat Diet, High fat diet and pulsed electromagnetic fields. Pulsed electromagnetic field was applied with pulsed electromagnetic energy therapy equipment. The Glucose, free fatty acids, triglycerides, cholesterol and insulin was used to measure hyperlipidemia-related blood components, and the GSH, GRD, XO and MDA was used to measure antioxidant enzymes and reactive oxygen. The applied pulsed electromagnetic field has improved the concentrations of blood components and increasing GSH and GRD, that were decreased due to a high-fat diet and by reducing the increased MDA and XO to a level of general diet group. Therefore, the applied pulsed electromagnetic field can be the effective treatment for changes in the blood composition of hyperlipidemic rats, enhancement of antioxidative activity and the inhibition of reactive oxygen.
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문제 정의
There have been lots of previous studies on the changes in blood components in patients with hyperlipidemia and changes in antioxidant enzymes and reactive oxygen depending on various methods of exercise, but studies are very limited on the effects of electrical stimulation such as pulsating electromagnetic fields. Therefore, the present study was aimed to investigate the effects of the application of pulsed electromagnetic fields on the blood components associated hyperlipidemia, antioxidant enzymes and reactive oxygen, and to provide basic data on the effects of pulsating electromagnetic field on the improvement of hyperlipidemia.
제안 방법
Following the 6 week application of pulsating electromagnetic field, the test rats were anesthetized with CO2 gas and under went the ventral midline laparotomy, and blood samples were taken from the abdominal aorta and immediately centrifuged at 3,000rpm at 4℃ for 20 minutes before removing the plasma, followed by being store at -80℃ for before analysis.
The animals used in this experiment are three week-old, white male Spraque-Dawley rats weighing 50g. For experiments, the subjects were divided into three groups: normal diet group consuming ordinary solid feed (General Diet; GD, n = 10); group consuming high fat diet which accounts for 51 percent of total calories (High fat Diet; HD, n = 10); and the group consuming high fat diet and applied with pulsed electromagnetic field (High fat diet + pulsed electromagnetic fields; HDP, n = 10). Each test group was fed with solid formulation feed for adaptation for one week before being fed with high fat feed for 8 weeks.
95%. Rats were put in a plastic case during the application to minimize the movement, for 20 minutes a time, 5 times a day, and a total of 6 weeks.
대상 데이터
The animals used in this experiment are three week-old, white male Spraque-Dawley rats weighing 50g. For experiments, the subjects were divided into three groups: normal diet group consuming ordinary solid feed (General Diet; GD, n = 10); group consuming high fat diet which accounts for 51 percent of total calories (High fat Diet; HD, n = 10); and the group consuming high fat diet and applied with pulsed electromagnetic field (High fat diet + pulsed electromagnetic fields; HDP, n = 10).
데이터처리
In addition, one-way ANOVA was conducted to verify the significance between each group, and Duncan's multiple range test was performed as a post-hoc comparison if proved significant between groups.
Statistical data analysis for this experiment was performed using the PASW statics windows (ver 18.0); the mean and standard deviation of the measured variables were calculated for each group. In addition, one-way ANOVA was conducted to verify the significance between each group, and Duncan's multiple range test was performed as a post-hoc comparison if proved significant between groups.
성능/효과
According to the result of antioxidant enzymes and reactive oxygen analysis, in the change of GSH and GRD related with the reaction of antioxidant enzymes, GSH showed lower value in high fat diet group than in normal diet group (p <.05), and was improved to the level of normal diet group by applying pulsed electromagnetic field (p <.05).
According to the result of hyperlipidemic blood components analysis, the plasma triglyceride level showed higher in high fat diet group than in normal diet group, demonstrating the induction of hypertriglyceridemia (p <.05), and was improved to the level of normal diet group by applying pulsed electromagnetic field (p <.05)[Table 1].
The lipid peroxidation generated in the cell membrane by reactive oxygen nonspecifically attacks the cell membrane and cause accumulation of the aging pigment that ultimately leads to cell death, and there is a report that the amount of lipid peroxidation cab be recognized by the measure of MDA [15]. It was found in this study that the adverse effects caused by reactive oxygen was higher in hyperlipidemia induced rats compared with normal rats because the high-fat diet group showed high levels of MDA than the normal diet group. However, as the results of applying pulsed electromagnetic field, it was found that the high MDA levels in the high-fat diet group were improved to the level of the normal diet group, which means the application of pulsed electromagnetic field has a positive effect on the inhibition of adverse effects caused by reactive oxygen.
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