[논문]PMFs Analysis of Krachaidum Products by HPLC and GC

Burapan, Supawadee; Kim, Mihyang; Han, Jaehong

doi:10.3839/jabc.2014.034

PMFs Analysis of Krachaidum Products by HPLC and GC 원문보기

Journal of applied biological chemistry, v.57 no.3, 2014년, pp.211 - 218

Burapan, Supawadee (Metalloenzyme Research Group and Department of Systems Biotechnology, Chung-Ang University) , Kim, Mihyang (Metalloenzyme Research Group and Department of Systems Biotechnology, Chung-Ang University) , Han, Jaehong (Metalloenzyme Research Group and Department of Systems Biotechnology, Chung-Ang University)

Abstract ▼ AI-Helper

Polymethoxyflavones (PMFs) are a group of polymethoxylated bioactive flavones with diverse biological activities, including anticancer, anti-inflammatory, antibacterial, and antiviral activities. PMFs are found from various plants such as orange, tangerine, and krachaidum. To establish the simple quantitative analytical methods for PMFs, chromatographic analysis was applied to the selected krachaidum foods because krachaidum contains diverse PMFs compared to other PMF-containing foods. Krachaidum is the rhizome of Kaempferia parviflora, and many commercial krachaidum products, such as tea, juice and wine, are commercially available and consumed as health functional foods in Asian countries. Apart from the claimed health promoting benefits, reliable quality assurance and legal guideline for the registration of these products are not available yet. Twelve PMFs were analyzed from the commercial krachaidum foods by GC-FID and HPLC-DAD. No single chromatographic method could not analyze 12 PMFs simultaneously. HPLC-DAD method was found more sensitive to detect PMFs. Based on our analysis data, we proposed 5,7-dimethoxyflaone and 5,7,4'-trimethoxyflavone as index components for the food products.

주제어

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문제 정의

This research reported a simple and efficient analyte preparation procedure from krachaidum foods for GC and HPLC analyses. The procedure can be applied to the PMFs analysis of any PMF-containing food products.

제안 방법

The GC-FID chromatogram of the chloroform analyte prepared from product 5 tea brewing were shown at Fig. 1. HP-5 column could not resolve PMF-9 and PMF-10 under various temperature gradient conditions and an isothermal GC conditions were optimized for PMFs analysis. GC analytes were dissolved in chloroform to suppress solvent peak.
In this study, we have established the PMFs analysis method with completely characterized 12 standard PMFs and two common chromatographic tools of HPLC and GC. Analytes from krachaidum foods were prepared differently, depending on the types of 16 krachaidum products.

데이터처리

All measurements, including analyte preparations, were done at least in triplicate. The data were processed to produce averages and standard deviations by Microsoft Excel.

성능/효과

2. C18 Hypersil GOLDΤΜ column-equipped HPLC analysis was able to resolve 12 PMFs, including 4'-hydroxy-5,7-dimethoxyflavone at 5.5 min which was not quantitated.
Composition analysis data suggested 5,7-dimethoxyflavone (PMF-1), 5,7,4'-trimethoxyflavone (PMF5), and 3,5,7,3',4'-pentamethoxyflavone (PMF-10) as index components, and these three PMFs can be used to set the guideline for the regulation and registration of krachaidum foods. The study also provided insights whether uptake of krachaidum foods actually increased the physiological concentration of PMFs to the level that exhibiting the specific biological activity.

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