Yam (Dioscorea batatas) Root and Bark Extracts Stimulate Osteoblast Mineralization by Increasing Ca and P Accumulation and Alkaline Phosphatase Activity원문보기
Yam (Dioscorea batatas) is widely consumed as functional food for health promotion mainly in East Asia countries. We assessed whether yam root (tuber) or bark (peel) extracts stimulated the activity of osteoblasts for osteogenesis. MC3T3-E1 cells (mouse osteoblasts) were treated with yam root extrac...
Yam (Dioscorea batatas) is widely consumed as functional food for health promotion mainly in East Asia countries. We assessed whether yam root (tuber) or bark (peel) extracts stimulated the activity of osteoblasts for osteogenesis. MC3T3-E1 cells (mouse osteoblasts) were treated with yam root extracts (water or methanol) (study I) or bark extracts (water or hexane) (study II) within $0{\sim}10{\mu}g/mL$ during the periods of osteoblast proliferation (5~10 day), matrix maturation (11~15 day) and mineralization (16~20 day) as appropriate. In study I, both yam root water and methanol extracts increased cell proliferation as concentration-dependent manner. Cellular collagen synthesis and alkaline phosphatase (ALP) activity, both the indicators of bone matrix protein and inorganic phosphate production for calcification respectively, were also increased by yam root water and methanol extract. Osteoblast calcification as cell matrix Ca and P accumulation was also increased by the addition of yam root extracts. In study II, yam bark extracts (water and hexane) increased osteoblast proliferation and differentiation, as collagen synthesis and ALP activity and osteoblast matrix Ca and P deposition. The study results suggested that both yam root and bark extracts stimulate osteogenic function in osteoblasts by stimulating bone matrix maturation by increasing collagen synthesis, ALP activity, and matrix mineralization.
Yam (Dioscorea batatas) is widely consumed as functional food for health promotion mainly in East Asia countries. We assessed whether yam root (tuber) or bark (peel) extracts stimulated the activity of osteoblasts for osteogenesis. MC3T3-E1 cells (mouse osteoblasts) were treated with yam root extracts (water or methanol) (study I) or bark extracts (water or hexane) (study II) within $0{\sim}10{\mu}g/mL$ during the periods of osteoblast proliferation (5~10 day), matrix maturation (11~15 day) and mineralization (16~20 day) as appropriate. In study I, both yam root water and methanol extracts increased cell proliferation as concentration-dependent manner. Cellular collagen synthesis and alkaline phosphatase (ALP) activity, both the indicators of bone matrix protein and inorganic phosphate production for calcification respectively, were also increased by yam root water and methanol extract. Osteoblast calcification as cell matrix Ca and P accumulation was also increased by the addition of yam root extracts. In study II, yam bark extracts (water and hexane) increased osteoblast proliferation and differentiation, as collagen synthesis and ALP activity and osteoblast matrix Ca and P deposition. The study results suggested that both yam root and bark extracts stimulate osteogenic function in osteoblasts by stimulating bone matrix maturation by increasing collagen synthesis, ALP activity, and matrix mineralization.
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가설 설정
In this study, we tested whether various yam root (study I) and bark extracts (study II) have the potential osteogenic activity by osteoblast function. We tested osteogenic activity by measuring synthesis of major bone matrix proteins, collagen (as bone matrix net-like protein for Ca and P deposition), and ALP (an enzyme producing inorganic phosphate for matrix mineral nucleation) and Ca and P deposition in cell layers.
제안 방법
For this reasons, MC3T3-E1 cells are in use for bone-formation study, such as to assess the osteogenic function of nutrients or phytochemicals (7,8). In this study, we used MC3T3-E1 cells as in vitro model for studying osteogenic effect of yam extracts.
데이터처리
The data analysis was performed using one way analysis of variance (ANOVA) and Tukey’s HSD test (P＜0.05), if any significance among means were observed.
MC3T3-E1 cells were cultured with yam bark H2O and hexane extracts (0∼10 μg/mL) up to 15 days and collagen concentration and ALP activity measured. Values were analyzed by Tukey as post hoc, one-way ANOVA. Different letters (a-c) mean significant difference among the groups.
MC3T3-E1 cells were cultured with 0~10 μg/mL yam extracts (H2O or MeOH) up to 15 days. Values were analyzed by one-way ANOVA, Tukey test for mean comparison as post hoc. Different letters (a-c) mean significant difference among the groups.
이론/모형
Cell proliferation was measured using MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] (Sigma) assay. MC3T3-E1 cells were cultured in 96-well plate and treated with 0∼10 μg/mL of yam root and bark extracts for 10 days.
Collagen in cellular and extracellular (media) were measured, after being treated with 0∼10 μg/mL yam root and bark extracts for 9 and 15 days. The concentration of collagen was measured using the Sircol soluble collagen assay. Briefly, each 50 μL of collagen standards and samples were added to 1.
To measure collagen accumulation, cell layers was stained by van Gieson’s method.
성능/효과
There was no significance between vehicle (0 μg of yam extract/mL, DMSO only) and normal OSM, which means no DMSO as solvent effect was accounted.
후속연구
In conclusion, we suggest that yam bark (H2O and hexane) extracts as well as yam root (H2O and MeOH) extracts has the beneficial effect for the osteogenesis in osteoblasts by stimulating osteoblast differentiation and cell matrix calcification. Further studies are needed for clarifying how and whereby the yam bark extracts and components stimulate osteoblast bone formation.
참고문헌 (28)
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