[논문]Ethanol Production from Glycerol by the Yeast Pachysolen tannophilus Immobilized on Celite during Repeated-Batch Flask Culture

Cha, Hye-Geun; Kim, Yi-Ok; Lee, Hyeon-Yong; Choi, Woon Yong; Kang, Do-Hyung; Jung, Kyung-Hwan

doi:10.5941/myco.2014.42.3.305

Ethanol Production from Glycerol by the Yeast Pachysolen tannophilus Immobilized on Celite during Repeated-Batch Flask Culture 원문보기

Mycobiology, v.42 no.3, 2014년, pp.305 - 309

Cha, Hye-Geun (Department of Biotechnology, Korea National University of Transportation) , Kim, Yi-Ok (Department of Biotechnology, Korea National University of Transportation) , Lee, Hyeon-Yong (Department of Food Science and Engineering, Seowon University) , Choi, Woon Yong (Department of Medical Biomaterials Engineering, Kangwon National University) , Kang, Do-Hyung (Korea Institute of Ocean Science and Technology) , Jung, Kyung-Hwan (Department of Biotechnology, Korea National University of Transportation)

Abstract ▼ AI-Helper

We investigated a novel process for production of ethanol from glycerol using the yeast Pachysolen tannophilus. After optimization of the fermentation medium, repeated-batch flask culture was performed over a period of 378 hr using yeast cells immobilized on Celite. Our results indicated that the use of Celite for immobilization of P. tannophilus was a practical approach for ethanol production from glycerol, and should be suitable for industrial ethanol production.

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문제 정의

In this study, we investigated a novel process for ethanol production from glycerol using the yeast P. tannophilus with the goal of developing a more practical process for ethanol production from glycerol. First, the medium for ethanol production from glycerol was optimized on the basis of the cost-effectiveness of the medium ingredients, such as the use of industrial-grade yeast extract and corn steep liquor (CSL).

제안 방법

Batch flask cultures using YCG2 medium (40 g/L glycerol) was performed (Fig. 3) by replacing the media three times to produce a series of four batch cultures using the two techniques described above. As shown in Fig.
tannophilus for ethanol production from glycerol. Finally, we evaluated the effectiveness of Celite as an immobilization carrier during repeated-batch culture, as compared to cell recycle using centrifugation.

대상 데이터

The yeast strain used in this study was P. tannophilus ATCC 32691. For the seed culture, the yeast was cultivated in a 100 mL Erlenmeyer flask in YPD (10 g/L yeast extract, 20 g/L peptone, and 20 g/L glucose) or YPG medium (10 g/L yeast extract, 20 g/L peptone, and 20 g/L glycerol), in an incubator shaker maintained at 30℃ and 150 rpm.

참고문헌 (18)

1 Abbaszaadeh A Ghobadian B Omidkhah MR Najafi G Current biodiesel production technologies: a comparative review Energy Convers Manag 2012 63 138 148
2 Salvi BL Panwar NL Biodiesel resources and production technologies: a review Renew Sustain Energy Rev 2012 16 3680 3689
3 Dobson R Gary V Rumbold K Microbial utilization of crude glycerol for the production of value-added products J Ind Microbiol Biotechnol 2012 39 217 226 21948485

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4 Rywiñska A Juszczyk P Wojtatowicz M Robak M Lazar Z Tomaszewska L Rymowicz W Glycerol as a promising substrate for Yarrowia lipolytica biotechnological applications Biomass Bioenergy 2013 48 148 166

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5 Clomburg JM Gonzalez R Anaerobic fermentation of glycerol: a platform for renewable fuels and chemicals Trends Biotechnol 2013 31 20 28 23178075
6 Liu X Jensen PR Workman M Bioconversion of crude glycerol feedstocks into ethanol by Pachysolen tannophilus Bioresour Technol 2012 104 579 586 22093973

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7 Maleszka R Wang PY Schneider H Ethanol production from D-galactose and glycerol by Pachysolen tannophilus Enzyme Microb Technol 1982 4 349 352

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8 Yazdani SS Gonzalez R Anaerobic fermentation of glycerol: a path to economic viability for the biofuels industry Curr Opin Biotechnol 2007 18 213 219 17532205

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9 Gancedo C Gancedo JM Sols A Glycerol metabolism in yeasts: pathways of utilization and production Eur J Biochem 1968 5 165 172 5667352

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10 Klingenberg M Localization of the glycerol-phosphate dehydrogenase in the outer phase of the mitochondrial inner membrane Eur J Biochem 1970 13 247 252 5439930

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11 Vieira AM Sá-Correia I Novais JM Cabral JM Could the improvements in the alcoholic fermentation of high glucose concentrations by yeast immobilization be explained by media supplementation? Biotechnol Lett 1989 11 137 140

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12 Khare SK Nakajima M Immobilization of Rhizopus japonicus lipase on celite and its application for enrichment of docosahexaenoic acid in soybean oil Food Chem 2000 68 153 157

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13 Lim HK Jung KH Park DH Chung SI Production characteristics of interferon-alpha using an L-arabinose promoter system in a high-cell-density culture Appl Microbiol Biotechnol 2000 53 201 208 10709983
14 Lee SE Lee CG Kang DH Lee HY Jung KH Preparation of corncob grits as a carrier for immobilizing yeast cells for ethanol production J Microbiol Biotechnol 2012 22 1673 1680 23221530

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15 Lee SE Kim YO Choi WY Kang DH Lee HY Jung KH Two-step process using the immobilized Saccharomyces cerevisiae and Pichia stipitis for ethanol production from the hydrolysate of Ulva pertusa Kjellman J Microbiol Biotechnol 2013 23 1434 1444 23851267
16 Lee SE Choi WS Kang DH Lee HY Jung KH Improvement of thin-layer chromatography for quantitative glycerol assay in biodiesel J Life Sci 2013 23 537 541
17 Yeon JH Lee SE Choi WY Kang DH Lee HY Jung KH Repeated-batch operation of surface-aerated fermentor for bioethanol production from the hydrolysate of seaweed Sargassum sagamianum J Microbiol Biotechnol 2011 21 323 331 21464605

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18 Kim YO Choi WY Kang DH Lee HY Jung KH Ethanol production from glycerol using Pachysolen tannophilus in a surface-aerated fermentor J Life Sci 2013 23 886 892

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Ethanol Production from Glycerol by the Yeast Pachysolen tannophilus Immobilized on Celite during Repeated-Batch Flask Culture 원문보기

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Ethanol Production from Glycerol by the Yeast Pachysolen tannophilus Immobilized on Celite during Repeated-Batch Flask Culture 원문보기

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