Chou, David Jiyao
(Division of Life Science and Center for Chinese Medicine, The Hong Kong University of Science and Technology)
,
Lam, Kelly Yinching
(Division of Life Science and Center for Chinese Medicine, The Hong Kong University of Science and Technology)
,
Chen, Jianping
(Division of Life Science and Center for Chinese Medicine, The Hong Kong University of Science and Technology)
,
Yao, Ping
(Division of Life Science and Center for Chinese Medicine, The Hong Kong University of Science and Technology)
,
Dong, Tina Tingxia
(Division of Life Science and Center for Chinese Medicine, The Hong Kong University of Science and Technology)
,
Xiong, Aizhen
(Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine)
,
Chou, Guixin
(Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine)
,
Wang, Zhengtao
(Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine)
,
Tsim, Karl Wah-Keung
(Division of Life Science and Center for Chinese Medicine, The Hong Kong University of Science and Technology)
Linderae Radix, the dry roots of Lindera aggregata (Sims) Kosterm, has long been used as traditional Chinese medicine for treatment of inflammatory diseases. The total alkaloids are believed to be the active components responsible for anti-inflammation of Linderae Radix. Here, the total alkaloids of...
Linderae Radix, the dry roots of Lindera aggregata (Sims) Kosterm, has long been used as traditional Chinese medicine for treatment of inflammatory diseases. The total alkaloids are believed to be the active components responsible for anti-inflammation of Linderae Radix. Here, the total alkaloids of Linderae Radix were extracted and isolated, including 12 isoquinoline alkaloids and 1 furan sesquiterpene. Within the alkaloids, norisoboldine, boldine, linderaline, isoboldine, reticuline, N-methyllaurotetanine, norjuziphine were found to be the major ingredients. In lipopolysaccharide-treated macrophage RAW 264.7 cells, application of Linderae Radix extract, or total alkaloids, suppressed the transcription of proinflammatory cytokines, interleukin-$1{\beta}$ and interleukin-6. Out of the 12 alkaloids, norisoboldine, boldine, and isoboldine were tested in lipopolysaccharide-treated macrophages, and norisoboldine was the strongest alkaloid in suppressing the cytokine expressions. The current studies suggested that the identification of alkaloids from Linderae Radix could provide a plausible explanation for herbal therapeutic functions.
Linderae Radix, the dry roots of Lindera aggregata (Sims) Kosterm, has long been used as traditional Chinese medicine for treatment of inflammatory diseases. The total alkaloids are believed to be the active components responsible for anti-inflammation of Linderae Radix. Here, the total alkaloids of Linderae Radix were extracted and isolated, including 12 isoquinoline alkaloids and 1 furan sesquiterpene. Within the alkaloids, norisoboldine, boldine, linderaline, isoboldine, reticuline, N-methyllaurotetanine, norjuziphine were found to be the major ingredients. In lipopolysaccharide-treated macrophage RAW 264.7 cells, application of Linderae Radix extract, or total alkaloids, suppressed the transcription of proinflammatory cytokines, interleukin-$1{\beta}$ and interleukin-6. Out of the 12 alkaloids, norisoboldine, boldine, and isoboldine were tested in lipopolysaccharide-treated macrophages, and norisoboldine was the strongest alkaloid in suppressing the cytokine expressions. The current studies suggested that the identification of alkaloids from Linderae Radix could provide a plausible explanation for herbal therapeutic functions.
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제안 방법
And quantitative real-time PCR was performed by Faststart Universal SYBR Green Master (ROX) (Roche Applied Science, Mannheim, Germany), according to the manufacturer’s instructions.
Statistical analysis (SPSS) was used to analyze the correlation of different bioactivity of isoquinoline alkaloids. Statistical tests were done by using one-way ANOVA.
대상 데이터
Chemical structures of major isolated products from total a lkaloids. The structures were identified by comparing their ESI-M S, 1H-NMR (400/500 MHz) and 13C-NMR (100/125 MHz) data. The identity of these chemicals have been reported in literatures.
The voucher specimens (WYS001-006) were deposited in Centre for Chinese Medicine at Hong Kong University of Science & Technology.
데이터처리
Statistical analysis (SPSS) was used to analyze the correlation of different bioactivity of isoquinoline alkaloids. Statistical tests were done by using one-way ANOVA. The data was separated into two groups to compare both the activation of tested compounds and control.
성능/효과
The findings indicated that both herbal extract and alkaloids from Linderae Radix might inhibit the LPS-induced IL-6 and IL-1β by blocking the phosphorylation of NF-κB subunits.
The results suggested that for each isoquinoline alkaloid, the doses below 30 μM did not show remarkable cytotoxicity or proliferation effect.
These results indicated that the ethanol extract, total alkaloids and few isoquinoline alkaloids from Linderae Radix showed inhibition against the LPS-enhanced gene expressions of proinflammatory cytokines, IL-6 and IL-β, on mRNA levels.
참고문헌 (24)
Bresnihan B. Pathogenesis of joint damage in rheumatoid arthritis. J Rheumatol. 1999;26:717-719.
Chou, GX, Nakamura, N, Ma, CM, Wang, ZT, Hattori, M. Isoquinoline alkaloids from Lindera aggregata. Chin J Nat Med. 2005;3:272-275.
Du CY, Choi RC, Zheng KY, Dong TT, Lau DT, Tsim KW. Yu Ping Feng San, an Ancient Chinese Herbal Decoction Containing Astragali Radix, Atractylodis Macrocephalae Rhizoma and Saposhnikoviae Radix, Regulates the Release of Cytokines in Murine Macrophages. PLoS One. 2013;8:e78622.
Luo Y, Liu M, Dai Y, Yao X, Xia Y, Chou G, & Wang Z. Norisoboldine inhibits the production of pro-inflammatory cytokines in lipopolysaccharide-stimulated RAW 264.7 cells by down-regulating the activation of MAPKs but not NF- $\kappa$ B. Inflammation. 2010;33:389-397.
Luo Y, Liu M, Xia Y, Dai Y, Chou G, Wang Z. Therapeutic effect of norisoboldine, an alkaloid isolated from Radix Linderae, on collagen-induced arthritis in mice. Phytomedicine. 2010;17:726-731.
Luo Y, Liu M, Yao X, Xia Y, Dai Y, Chou G, & Wang Z. Total alkaloids from Radix Linderae prevent the production of inflammatory mediators in lipopolysaccharide-stimulated RAW 264.7 cells by suppressing NF- $\kappa$ B and MAPKs activation. Cytokine. 2009;46:104-110.
Tak PP, Bresnihan B. The pathogenesis and prevention of joint damage in rheumatoid arthritis: advances from synovial biopsy and tissue analysis. Arthritis Rheum. 2000;43:2619-2633.
Xiao Y, Li Z Y, Li L. Chemical constituents (II) from Litsea euosma W. W. Smith. Yunnan Chemical Technology. 2006;33:22-23.
Xiao Y, Yang XD, Zhou HJ, Zhao JF, Yang JH, Li L. Isoquinoline alkaloids from Litsea euosma. Journal of Yunnan University. 2004;26:192-193. http://www.cqvip.com/qk/92787x/2004b07/10058518.html
Winer J, Jung CK, Shackel I, Williams PM. Development and validation of real-time quantitative reverse transcriptasepolymerase chain reaction for monitoring gene expression in cardiac myocytes in vitro. Anal Biochem. 1999;270:41-49.
Yu R, Ye Q, Chen B, Zhang GL. Chemical study on Mitrephora Natural Product Research and Development. 2003;15:212-215.
Zhu CL, Yang PM. Isolation and structure identification of chemical constituents from the root of Litsea cubeba. Chinese Journal of Pharmaceuticals. 2007;38:558-560. http://www.cqvip.com/qk/92282x/200708/25126172.html
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