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NTIS 바로가기생명과학회지 = Journal of life science, v.24 no.11 = no.175, 2014년, pp.1258 - 1267
Developing economic traits with a high growth rate, robustness, and disease resistance in livestock is an important challenge. RFLP and AFLP are the classical methods used to develop economic traits. Whole-genome-based economic traits have recently been detected with the advent of next-generation se...
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핵심어 | 질문 | 논문에서 추출한 답변 |
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RFLP 기법을 통한 유전자 마커 발굴의 한계를 극복한 방법은 무엇인가? | 이전에는 RFLP (Restriction Fragment Length Polymorphism), AFLP (Amplified Fragment Length Polymorphism), microsatellite와 같은 기법을 사용해서 개체군 간 SNP (Single Nucleotide Polymorphism) 및 CNV (Copy Number Variation)와 같은 다양한 유전자 마커를 발굴하고 있었으나[13, 68], 이러한 기법들은 상대적으로 적은 정보와 많은 노동력 및 연구비용을 필요로 한다. 현재, NGS기법의 대두로 인하여 시퀀스 정보와 유전형질이 많은 양의 데이터로 발굴되고 있는 실정이다. 이러한 NGS 데이터가 많이 쏟아져 나옴에 따라 기능유전체 분야의 연구가 가속화되고 있고, 인간 유전체에서 ENCODE (Encyclopedia of DNA Elements) 프로젝트가 주목받고 있다[15]. | |
유전자 마커란 무엇인가? | 유전자 마커란 인간을 비롯한 각 생명체의 표현형질을 나타내는 유전적 특성을 말한다[16, 18] 지금까지 인간에 있어서 정상과 질병 샘플을 비교한 연구가 많이 진행되었으며[49, 50], 가축에 있어서는 각 개체군에서 우수한 경제형질을 가진 샘플과 그렇지 않은 샘플을 비교한 연구가 많이 진행되었다[17, 25]. 이러한 표현형질을 나타내는 유전자 마커는 생명체의 게놈 상에 존재하고 있으나, 전장게놈의 경우 매우 방대하기에 기본적으로 형질과 관련되어 있다고 알려진 중요한 유전자 위주로 연구되고 있었다. | |
유전자 마커는 무엇을 위주로 연구되고 있는가? | 유전자 마커란 인간을 비롯한 각 생명체의 표현형질을 나타내는 유전적 특성을 말한다[16, 18] 지금까지 인간에 있어서 정상과 질병 샘플을 비교한 연구가 많이 진행되었으며[49, 50], 가축에 있어서는 각 개체군에서 우수한 경제형질을 가진 샘플과 그렇지 않은 샘플을 비교한 연구가 많이 진행되었다[17, 25]. 이러한 표현형질을 나타내는 유전자 마커는 생명체의 게놈 상에 존재하고 있으나, 전장게놈의 경우 매우 방대하기에 기본적으로 형질과 관련되어 있다고 알려진 중요한 유전자 위주로 연구되고 있었다. 이전에는 RFLP (Restriction Fragment Length Polymorphism), AFLP (Amplified Fragment Length Polymorphism), microsatellite와 같은 기법을 사용해서 개체군 간 SNP (Single Nucleotide Polymorphism) 및 CNV (Copy Number Variation)와 같은 다양한 유전자 마커를 발굴하고 있었으나[13, 68], 이러한 기법들은 상대적으로 적은 정보와 많은 노동력 및 연구비용을 필요로 한다. |
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