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Journal of embryo transfer = 한국수정란이식학회지, v.30 no.3, 2015년, pp.121 - 128
이경진 (강원대학교 동물생명과학대학) , 이상희 (강원대학교 동물생명과학대학) , 주선호 (강원대학교 동물생명과학대학) , 김유진 (강원대학교 동물생명과학대학) , 양진우 (강원대학교 동물생명과학대학) , 이연주 (강원대학교 동물생명과학대학) , 황보용 (강원대학교 동물생명과학대학) , 이승형 (강원대학교 동물생명과학대학) , 이승태 (강원대학교 동물생명과학대학) , 이은송 (강원대학교 수의과대학) , 박춘근 (강원대학교 동물생명과학대학)
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The objective of this study was to evaluate the efficiency of sperm cryosurvival in boar sperm separated by Percoll containing antioxidant enzymes. The boar semen was collected into a pre-warmed (
| 핵심어 | 질문 | 논문에서 추출한 답변 |
|---|---|---|
| 정액의 동결 보존은 어떠한 특징을 가지고 있는 기술인가? | 정액의 동결 보존은 정자를 초저온에서 보관하는 기술로, 반영구적인 보관이 가능하여 우수한 형질의 유전자원을 보존할 수 있는 특징을 가지고 있다. 이러한 장점으로 멸종위기동물의 유전자원 보존과 가축의 개량 및 생산성 향상에 크게영향을 미쳤다(Sieme와 Oldenhof, 2015). | |
| 정액의 동결과정 중 다양한 물리적, 화학적 스트레스에 노출되어 세포에서 생성되는 과도한 활성 산소는 정자에 어떤 영향을 미치는가? | 특히 돼지 정자는 다른 종에 비해 세포막 내 다중불포화지방산의 농도가 높기 때문에 산화적 충격에 더 민감하다(Strzezek 등, 2004). 이러한 활성 산소는 정자의 생존율과 운동성의 감소, 세포막 손상 및 DNA 손상에 영향을 미치게 되어, 최종적으로 정자의 수정능력을 감소시키게 된다(Knox 등, 2015). | |
| 돼지 정자가 산화적 충격에 더 민감한 이유는? | 동결과정 중 다양한물리적, 화학적 스트레스에 노출되어 세포에서 생성되는 과도한 활성 산소 종에 의해 정자가 손상을 받게 된다(Santiani 등, 2014). 특히 돼지 정자는 다른 종에 비해 세포막 내 다중불포화지방산의 농도가 높기 때문에 산화적 충격에 더 민감하다(Strzezek 등, 2004). 이러한 활성 산소는 정자의 생존율과 운동성의 감소, 세포막 손상 및 DNA 손상에 영향을 미치게 되어, 최종적으로 정자의 수정능력을 감소시키게 된다(Knox 등, 2015). |
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