[국내논문]Antibacterial activities of bark extracts from Fraxinus rhynchophylla Hance and Geranium koreanum Kom. against clinical strains of Clostridium perfringens in chickens원문보기
Oh, Jae-Young
(Department of Infectious Diseases and Avian Diseases, College of Veterinary Medicine and Korea Zoonosis Research Institute, Chonbuk National University)
,
Lee, Kwang-Jae
(Gangwon Agricultural Research and Extension Services)
,
Wei, Bai
(Department of Infectious Diseases and Avian Diseases, College of Veterinary Medicine and Korea Zoonosis Research Institute, Chonbuk National University)
,
Roh, Jae-Hee
(Department of Infectious Diseases and Avian Diseases, College of Veterinary Medicine and Korea Zoonosis Research Institute, Chonbuk National University)
,
Kang, Min
(Department of Infectious Diseases and Avian Diseases, College of Veterinary Medicine and Korea Zoonosis Research Institute, Chonbuk National University)
,
Cha, Se-Yeoun
(Department of Infectious Diseases and Avian Diseases, College of Veterinary Medicine and Korea Zoonosis Research Institute, Chonbuk National University)
,
Jang, Hyung-Kwan
(Department of Infectious Diseases and Avian Diseases, College of Veterinary Medicine and Korea Zoonosis Research Institute, Chonbuk National University)
Necrotic enteritis (NE) caused by Clostridium (C.) perfringens commonly occurs in domestic broiler farms since antibiotic supplementation in poultry feed has been banned. We evaluated the antibacterial activities of medicinal plant extracts against C. perfringens isolates to select alternative compo...
Necrotic enteritis (NE) caused by Clostridium (C.) perfringens commonly occurs in domestic broiler farms since antibiotic supplementation in poultry feed has been banned. We evaluated the antibacterial activities of medicinal plant extracts against C. perfringens isolates to select alternative compounds for preventing NE. We compared antibacterial activities using two methods and evaluated susceptibilities of the isolates based on minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Two (Fraxinus rhynchophylla Hance [FRH] and Geranium koreanum Kom. [GKK]) of the 30 plant extracts had potent antibacterial activities against C. perfringens ATCC 13124 in two assays. The MIC values for FRH and GKK against 20 C. perfringens isolates were $128{\sim}256{\mu}g/mL$ and $32{\sim}128{\mu}g/mL$, respectively. The geometric MIC mean values for the two extracts were $147.2{\mu}g/mL$ and $68.8{\mu}g/mL$, respectively. The MBCs for the two extracts against the same strains were $1,024{\sim}2,048{\mu}g/mL$ and $256{\sim}1,024{\mu}g/mL$, respectively. The geometric mean MIC and MBC for GKK were about two-fold lower than those of FRH. The modified spot-on-lawn assay may be useful for measuring primary antibacterial potential. FRH and GKK are expected to be used as feed additives to prevent or treat NE in veterinary practice.
Necrotic enteritis (NE) caused by Clostridium (C.) perfringens commonly occurs in domestic broiler farms since antibiotic supplementation in poultry feed has been banned. We evaluated the antibacterial activities of medicinal plant extracts against C. perfringens isolates to select alternative compounds for preventing NE. We compared antibacterial activities using two methods and evaluated susceptibilities of the isolates based on minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Two (Fraxinus rhynchophylla Hance [FRH] and Geranium koreanum Kom. [GKK]) of the 30 plant extracts had potent antibacterial activities against C. perfringens ATCC 13124 in two assays. The MIC values for FRH and GKK against 20 C. perfringens isolates were $128{\sim}256{\mu}g/mL$ and $32{\sim}128{\mu}g/mL$, respectively. The geometric MIC mean values for the two extracts were $147.2{\mu}g/mL$ and $68.8{\mu}g/mL$, respectively. The MBCs for the two extracts against the same strains were $1,024{\sim}2,048{\mu}g/mL$ and $256{\sim}1,024{\mu}g/mL$, respectively. The geometric mean MIC and MBC for GKK were about two-fold lower than those of FRH. The modified spot-on-lawn assay may be useful for measuring primary antibacterial potential. FRH and GKK are expected to be used as feed additives to prevent or treat NE in veterinary practice.
* AI 자동 식별 결과로 적합하지 않은 문장이 있을 수 있으니, 이용에 유의하시기 바랍니다.
제안 방법
The plant extract concentration ranges were set arbitrarily to compare the two experiments. Second, the spoton-lawn assay was used as described previously [12] with a slight modification. Briefly, 1 mL bacterial culture refreshed in MH broth was dropped to moisten the cultures on MH agar, and the remaining cultures were removed and placed in medium to dry for 10 min on a clean bench.
perfringens. The paper disc diffusion and the modified spoton-lawn assays were conducted by measuring inhibition zones around paper discs and clear inhibition zones of the dropped plant extract on culture medium, respectively.
이론/모형
MICs and MBCs were evaluated using a CLSI method [5] to determine the antimicrobial susceptibility of the two plant extracts. The MIC is the smallest concentration of an antibacterial agent that will inhibit growth of bacteria after an overnight incubation, and the MBC is the smallest concentration of antimicrobial that prevents growth of bacteria after subculture on antibiotic-free medium.
참고문헌 (34)
Bigos M, Wasiela M, Kalemba D, Sienkiewicz M. Antimicrobial activity of geranium oil against clinical strains of Staphylococcus aureus. Molecules 2012, 17, 10276-10291.
de Souza SM, Delle Monache F, Smania A Jr. Antibacterial activity of coumarins. Z Naturforsch C 2005, 60, 693-700.
Gholamiandehkordi A, Eeckhaut V, Lanckriet A, Timbermont L, Bjerrum L, Ducatelle R, Haesebrouck F, Van Immerseel F. Antimicrobial resistance in Clostridium perfringens isolates from broilers in Belgium. Vet Res Commun 2009, 33, 1031-1037.
Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Susceptibility Testing; Twentyfirst Informational Supplement. CLSI document M100-S21. Clinical and Laboratory Standards Institute, Wayne, 2011.
Jang SI, Jun MH, Lillehoj HS, Dalloul RA, Kong IK, Kim S, Min W. Anticoccidial effect of green tea-based diets against Eimeria maxima. Vet Parasitol 2007, 144, 172-175.
Jeon JR. Water extract of ash tree (Fraxinus rhynchophylla) leaves protects against paracetamol-induced oxidative damages in mice. Food Sci Biotechnol 2006, 15, 612-616.
Jeon JR, Choi JH. Protective effect of water extract of Fraxinus rhynchophylla leaves on acetaminophen-induced nephrotoxicity in mice and its phenolic compounds. Food Sci Biotechnol 2007, 16, 988-993.
Bae JH. Antimicrobial effect of Fraxinus rhynchophylla extracts on food-borne pathogens. Korean J Microbiol Biotechnol 2004, 32, 277-281.
Johansson A, Greko C, Engstrom BE, Karlsson M. Antimicrobial susceptibility of Swedish, Norwegian and Danish isolates of Clostridium perfringens from poultry, and distribution of tetracycline resistance genes. Vet Microbiol 2004, 99, 251-257.
Kim CH, Ji GE, Ahn C. Purification and molecular characterization of a bacteriocin from Pediococcus sp. KCA1303-10 isolated from fermented flatfish. Food Sci Biotechnol 2000, 9, 270-276.
Knarreborg A, Simon MA, Engberg RM, Jensen BB, Tannock GW. Effects of dietary fat source and subtherapeutic levels of antibiotic on the bacterial community in the ileum of broiler chickens at various ages. Appl Environ Microbiol 2002, 68, 5918-5924.
Kwon YS, Kim CM. A study on the chemical constituents from leaves of Fraxinus rhynchophylla. Korean J Pharmacogn 1996, 27, 347-349.
Lee JJ, Kim DH, Lim JJ, Kim DG, Min W, Kim GS, Lee HJ, Rhee MH, Park H, Kim SC, Chang HH, Kim S. Anticoccidial effect of supplemental dietary Galla Rhois against infection with Eimeria tenella in chickens. Avian Pathol 2012, 41, 403-407.
Lima ET, Andreatti Filho RL, Okamoto AS, Noujaim JC, Barros MR, Crocci AJ. Evaluation in vitro of the antagonistic substances produced by Lactobacillus spp. isolated from chickens. Can J Vet Res 2007, 71, 103-107.
Odey MO, Iwara IA, Udiba UU, Johnson JT, Inekwe UV, Asenye ME, Victor O. Preparation of plant extracts from indigenous medicinal plants. Int J Sci Technol 2012, 1, 688-692.
Ohmomo S, Murata S, Katayama N, Nitisinprasart S, Kobayashi M, Nakajima T, Yajima M, Nakanishi K. Purification and some characteristics of enterocin ON-157, a bacteriocin produced by Enterococcus faecium NIAI 157. J Appl Microbiol 2000, 88, 81-89.
Olkowski AA, Wojnarowicz C, Chirino-Trejo M, Drew MD. Responses of broiler chickens orally challenged with Clostridium perfringens isolated from field cases of necrotic enteritis. Res Vet Sci 2006, 81, 99-108.
Radulovic NS, Zlatkovic D, Dekic M, Stojanovic-Radi Z. Further antibacterial Geranium macrorrhizum L. metabolites and synthesis of epoxygermacrones. Chem Biodivers 2014, 11, 542-550.
Rattanachaikunsopon P, Phumkhachorn P. A bacteriocin produced by Lactobacillus lactis subsp. lactis isolated from Thai fermented foods. Sci Asia 2000, 26, 195-200.
Hahn DR, Kim CJ, Kim JH, Hong SK. Coumarin glycoside of Fraxinus chiisanensis. Korean J Pharmacogn 1976, 7, 195-198.
Slavic D, Boerlin P, Fabri M, Klotins KC, Zoethout JK, Weir PE, Bateman D. Antimicrobial susceptibility of Clostridium perfringens isolates of bovine, chicken, porcine, and turkey origin from Ontario. Can J Vet Res 2011, 75, 89-97.
Song KD, Lee WK. Antibacterial activity of recombinant pig intestinal parasite cecropin P4 peptide secreted from Pichia pastoris. Asian-Australas J Anim Sci 2014, 27, 278-283.
Timbermont L, Haesebrouck F, Ducatelle R, Van Immerseel F. Necrotic enteritis in broilers: an updated review on the pathogenesis. Avian Pathol 2011, 40, 341-347.
van Asten AJAM, van der Wiel CW, Nikolaou G, Houwers DJ, Grone A. A multiplex PCR for toxin typing of Clostridium perfringens isolates. Vet Microbiol 2009, 136, 411-412.
Yang EJ, Lee DG, Lee JW, Kim YS, Lim SH, Song KS. The chemical constituents of the stem barks of Fraxinus rhynchophylla. J Korean Soc Appl Biol Chem 2007, 50, 348-351.
Yurdugul S, Bozoglu F. Studies on an inhibitor produced by lactic acid bacteria of wines on the control of malolactic fermentation. Eur Food Res Technol 2002, 215, 38-41.
Zhang DF, Sun BB, Yue YY, Zhou QJ, Du AF. Anticoccidial activity of traditional Chinese herbal Dichroa febrifuga Lour. extract against Eimeria tenella infection in chickens. Parasitol Res 2012, 111, 2229-2233.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.