[논문]Zerumbone, Sesquiterpene Photochemical from Ginger, Inhibits Angiogenesis

Park, Ju-Hyung; Park, Geun Mook; Kim, Jin-Kyung

doi:10.4196/kjpp.2015.19.4.335

Zerumbone, Sesquiterpene Photochemical from Ginger, Inhibits Angiogenesis 원문보기

The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology, v.19 no.4, 2015년, pp.335 - 340

Park, Ju-Hyung (Department of Biomedical Science, Catholic University of Daegu) , Park, Geun Mook (Department of Biomedical Science, Catholic University of Daegu) , Kim, Jin-Kyung (Department of Biomedical Science, Catholic University of Daegu)

Abstract ▼ AI-Helper

Here, we investigated the role of zerumbone, a natural cyclic sesquiterpene of Zingiber zerumbet Smith, on angiogenesis using human umbilical vein endothelial cells (HUVECs). Zerumbone inhibited HUVECs proliferation, migration and tubule formation, as well as angiogenic activity by rat aorta explants. In particular, zerumbone inhibited phosphorylation of vascular endothelial growth factor receptor-2 and fibroblast growth factor receptor-1, which are key regulators of endothelial cell function and angiogenesis. In vivo matrigel plug assay in mice demonstrated significant decrease in vascularization and hemoglobin content in the plugs from zerumbone-treated mice, compared with control mice. Overall, these results suggest that zerumbone inhibits various attributes of angiogenesis, which might contribute to its reported antitumor effects.

주제어

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제안 방법

To determine of cytotoxicity of zerumbone on HUVECs, the LDH release assay was performed using a CytoTox 96Ⓡ Non-Radioactive Cytotoxicity assay kits according to the manufacturer’s instructions.
Endothelial migration is the key process in angiogenesis. To determine whether zerumbone could inhibit endothelial cell migration, wound-healing assay was employed to explore the changes in migration. As shown in Fig.

대상 데이터

Four-week-old C57BL/6 male mice were obtained from Hyochang Science and were subcutaneously injected with 500 μl of matirgel containing 100 ng/ml of recombinant mouse VEGF164, 100 ng/ml of recombinant mouse bFGF and 25 U heparin with indicated concentration of zerumbone.
Anti-phospho- FGFR antibody was obtained from R&D systems (Minneapolis, MN, USA). Recombinant human VEGF₁₆₅ and bFGF were purchased from Pepprotech (Rocky Hill, NJ, USA).
. Thoracic aortas were excised from 8-week-old male Sprague-Dawley rats (Hyochang Science, Deagu, Korea), and the fibroadipose tissue was removed. The aortas were sectioned into 1 mm to 1.

데이터처리

The data are means±SEM of at least three independent experiments. The values were evaluated by one-way analysis of variance (ANOVA) with Bonferroni multiple comparison post tests using GraphPad Prism 4.0 software (Graph-Pad Software Inc., San Diego, CA). p-values＜0.

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