선택한 단어 수는 입니다.
최소 단어 이상 선택하여야 합니다.
최소 단어 이상 선택하여야 합니다.
선택한 단어 수는 30입니다.
최대 10 단어까지만 선택 가능합니다.
최대 10 단어까지만 선택 가능합니다.
제안 방법
- 5% agarose gel electrophoresis, followed by purification using a PCR purification kit (Core-one; Core-Bio, Seoul, Korea). Reactions were sequenced using the BigDye Terminator Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA) and analyzed on an ABI 3130 Automated DNA Sequencer (Applied Biosystems). The identity of the isolates was established by comparing their ITS and TEF sequences with those in the GenBank database (National Center for Biotechnology Information [NCBI] US National Institute of Health, Bethesda, MD, USA; http://www.
대상 데이터
- For morphological observation, a small piece of living tissue containing fungal structures was mounted in a drop of water. Bright-field and differential-interference contrast light microscopy (Zeiss AX10 microscope; Zeiss, Jena, Germany) were used to observe the specimen. Measurements of the fungal structures were performed at 200~1,000×magnification.
- The ITS and TEF sequences from a representative isolate were deposited in GenBank with the accession numbers KF372590 (535 bp) and KF372592 (679 bp). A BLAST search of the ITS sequence in GenBank yielded 100% nucleotide identity with the F.
- Reactions were sequenced using the BigDye Terminator Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA) and analyzed on an ABI 3130 Automated DNA Sequencer (Applied Biosystems). The identity of the isolates was established by comparing their ITS and TEF sequences with those in the GenBank database (National Center for Biotechnology Information [NCBI] US National Institute of Health, Bethesda, MD, USA; http://www.ncbi.nlm.nih.gov/BLAST) and FUSARIUM ID (http://isolate.fusariumdb.org/blast.php), respectively. Phylogenetic analysis of ITS and TEF was conducted by neighbor-joining methods using MEGA version 6.
- After drying, we cultured the samples in 90-mm petri dishes containing potato dextrose agar (Difco, Sparks, MD, USA) in the dark at 25℃ for 1 wk. Voucher specimens were deposited in the Korea University Herbarium (KUS). Three isolates were obtained from vascular tissue of the infected plants.
이론/모형
- php), respectively. Phylogenetic analysis of ITS and TEF was conducted by neighbor-joining methods using MEGA version 6.0 [7], and the sequence distance was calculated based on the Tamura-Nei parameter model. Colletotrichum gloeosporioides (DQ084495) was used as an outgroup in the phylogenetic analysis.
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