Kim, Min Jeong
(Organic Agriculture Division, National Academy of Agricultural Science, Rural Development Administrations)
,
Shim, Chang Ki
(Organic Agriculture Division, National Academy of Agricultural Science, Rural Development Administrations)
,
Kim, Yong Ki
(Organic Agriculture Division, National Academy of Agricultural Science, Rural Development Administrations)
,
Hong, Sung Jun
(Organic Agriculture Division, National Academy of Agricultural Science, Rural Development Administrations)
,
Park, Jong Ho
(Organic Agriculture Division, National Academy of Agricultural Science, Rural Development Administrations)
,
Han, Eun Jung
(Organic Agriculture Division, National Academy of Agricultural Science, Rural Development Administrations)
,
Kim, Jin Ho
(Organic Agriculture Division, National Academy of Agricultural Science, Rural Development Administrations)
,
Kim, Suk Chul
(Organic Agriculture Division, National Academy of Agricultural Science, Rural Development Administrations)
This study investigated the chemical characteristics and microbial population during incubation of four kinds of aerated compost teas based on oriental medicinal herbs compost, vermicompost, rice straw compost, and mixtures of three composts (MOVR). It aimed to determine the effects of the aerated c...
This study investigated the chemical characteristics and microbial population during incubation of four kinds of aerated compost teas based on oriental medicinal herbs compost, vermicompost, rice straw compost, and mixtures of three composts (MOVR). It aimed to determine the effects of the aerated compost tea (ACT) based on MOVR on the growth promotion of red leaf lettuce, soybean and sweet corn. Findings showed that the pH level and EC of the compost tea slightly increased based on the incubation time except for rice straw compost tea. All compost teas except for oriental medicinal herbs and rice straw compost tea contained more ${NO^-}_3-N$ than ${NH^+}_4-N$. Plate counts of bacteria and fungi were significantly higher than the initial compost in ACT. Microbial communities of all ACT were predominantly bacteria. The dominant bacterial genera were analyzed as Bacillus (63.0%), Ochrobactrum (13.0%), Spingomonas (6.0%) and uncultured bacterium (4.0%) by 16S rDNA analysis. The effect of four concentrations, 0.1%, 0.2%, 0.4% and 0.8% MOVR on the growth of red leaf lettuce, soybean and sweet corn was also studied in the greenhouse. The red leaf lettuce with 0.4% MOVR had the most effective concentration on growth parameters in foliage part. However, 0.8% MOVR significantly promoted the growth of root and shoot of both soybean and sweet corn. The soybean treated with higher MOVR concentration was more effective in increasing the root nodule formation by 7.25 times than in the lower MOVR concentrations Results indicated that ACT could be used as liquid nutrient fertilizer with active microorganisms for culture of variable crops under organic farming condition.
This study investigated the chemical characteristics and microbial population during incubation of four kinds of aerated compost teas based on oriental medicinal herbs compost, vermicompost, rice straw compost, and mixtures of three composts (MOVR). It aimed to determine the effects of the aerated compost tea (ACT) based on MOVR on the growth promotion of red leaf lettuce, soybean and sweet corn. Findings showed that the pH level and EC of the compost tea slightly increased based on the incubation time except for rice straw compost tea. All compost teas except for oriental medicinal herbs and rice straw compost tea contained more ${NO^-}_3-N$ than ${NH^+}_4-N$. Plate counts of bacteria and fungi were significantly higher than the initial compost in ACT. Microbial communities of all ACT were predominantly bacteria. The dominant bacterial genera were analyzed as Bacillus (63.0%), Ochrobactrum (13.0%), Spingomonas (6.0%) and uncultured bacterium (4.0%) by 16S rDNA analysis. The effect of four concentrations, 0.1%, 0.2%, 0.4% and 0.8% MOVR on the growth of red leaf lettuce, soybean and sweet corn was also studied in the greenhouse. The red leaf lettuce with 0.4% MOVR had the most effective concentration on growth parameters in foliage part. However, 0.8% MOVR significantly promoted the growth of root and shoot of both soybean and sweet corn. The soybean treated with higher MOVR concentration was more effective in increasing the root nodule formation by 7.25 times than in the lower MOVR concentrations Results indicated that ACT could be used as liquid nutrient fertilizer with active microorganisms for culture of variable crops under organic farming condition.
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제안 방법
The compost tea extraction ratio was 1:1 (v/v) aerated compost tea to tap water and 50 mL of aerated compost tea was applied to the root zone and was applied to the foliage. Aerated compost teas were prepared separately events every week before application using the same batch of the mixture of four compost materials in a shade close to the greenhouse. Plants were harvested five weeks after treatment.
25 U of AmpliTaq DNA polymerase LD (Applied Biosystems, Rotkreuz, Switzerland), and the appropriate buffer. Amplicons were purified with the QIAquick PCR purification kit (Qiagen AG, Basel, Switzerland) and sequenced with forward primer fD1 using the BigDye kit and an automatic ABI Prism 3730 Genetic Analyzer (Applied Biosystems, Rotkreuz, Switzerland).
Experiments were conducted using a completely randomized design with 15 replicates of each treatment. Pairwise comparisons between treatments were performed using the least significant difference test.
The objectives of this study was to determine the efficacy dose of application and evaluated the efficacy of aerated compost teas prepared from oriental medicinal herbs compost, vermicompost, rice straw compost, and mixtures of three composts (MOVR) on the growth promotion of red leaf lettuce, sweet corn and soybean in the greenhouse.
Plants were harvested five weeks after treatment. The quantitative properties of red leaf lettuce, sweet corn, and soybean were determined in each plant by measuring the fresh weight and leaf number, leaf length, leaf width or shoot, and root length of the tested crops.
, Rotkreuz, Switzerland). The sequences were manually checked for quality, assembled, trimmed, and aligned using BioEdit (http://www.mbio.ncsu.edu/bioedit/bioedit.html). The sequences were subjected to Blast searches against the NCBI database.
데이터처리
Experiments were conducted using a completely randomized design with 15 replicates of each treatment. Pairwise comparisons between treatments were performed using the least significant difference test. When significant differences were observed (p≤0.
When significant differences were observed (p≤0.001), treatment means experiments were separated using Fisher’s least significant differences (LSD) test at 95% confidence level (p≤0.05) using the Statistical Analysis System, version 9.1 (SAS statistical analysis software, SAS Institute Inc. Cary, NC).
성능/효과
In the study, application of aerated compost tea from organic compost based using MOVR (the mixture of rice straw compost, vermicompost, and Hinoki cypress bark compost) to the root zone increased the plant shoot and root growths and yield of the red leaf lettuce, sweet corn, and soybean. Thus, compost tea could be used as an agent for promoting plant growth in organic cultivation of crops.
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