Ugurluoglu, Ceyhan
(Department of Pathology Disease, Selcuk University Medicine Faculty)
,
Kurtipek, Ercan
(Department of Chest Disease, Konya Training and Research Hospital)
,
Unlu, Yasar
(Department of Pathology Disease, Konya Training and Research Hospital)
,
Esme, Hidir
(Department of Thoracic Surgery, Konya Training and Research Hospital)
,
Duzgun, Nuri
(Department of Thoracic Surgery, Konya Training and Research Hospital)
Background: Cytological examination of pleural effusions is very important in the diagnosis of malignant lesions. Thoracentesis is the first investigation to be performed in a patient with pleural effusion. In this study, we aimed to compare traditional with cell block methods for diagnosis of lung ...
Background: Cytological examination of pleural effusions is very important in the diagnosis of malignant lesions. Thoracentesis is the first investigation to be performed in a patient with pleural effusion. In this study, we aimed to compare traditional with cell block methods for diagnosis of lung disease accompanied by pleural effusion. Materials and Methods: A total of 194 patients with exudative pleural effusions were included. Ten mililiters of fresh pleural fluid were obtained by thoracentesis from all patients in the initial evaluation. The samples gathered were divided to two equal parts, one for conventional cytological analysis and the other for analysis with the cell block technique. In cytology, using conventional diagnostic criteria cases were divided into 3 categories, benign, malignant and undetermined. The cell block sections were evaluated for the presence of single tumor cells, papillary or acinar patterns and staining with mucicarmine. In the cell block examination, in cases with sufficient cell counts histopathological diagnosis was performed. Results: Of the total undergoing conventional cytological analyses, 154 (79.4%)were reported as benign, 33 (17%) as malignant and 7 (3.6%) as suspicious of malignancy. With the cell block method the results were 147 (75.8%) benign, 12 (6.2%) metastatic, 4 (2.1%) squamous cell carcinoma, 18 (9.3%) adenocarcinoma, 5 (2.6%) large cell carcinoma, 2 (1%) mesothelioma, 3 (1.5%) small cell carcinoma, and 3 (1.5%) lymphoma. Conclusions: Our study confirmed that the cell block method increases the diagnostic yield with exudative pleural effusions accompanying lung cancer.
Background: Cytological examination of pleural effusions is very important in the diagnosis of malignant lesions. Thoracentesis is the first investigation to be performed in a patient with pleural effusion. In this study, we aimed to compare traditional with cell block methods for diagnosis of lung disease accompanied by pleural effusion. Materials and Methods: A total of 194 patients with exudative pleural effusions were included. Ten mililiters of fresh pleural fluid were obtained by thoracentesis from all patients in the initial evaluation. The samples gathered were divided to two equal parts, one for conventional cytological analysis and the other for analysis with the cell block technique. In cytology, using conventional diagnostic criteria cases were divided into 3 categories, benign, malignant and undetermined. The cell block sections were evaluated for the presence of single tumor cells, papillary or acinar patterns and staining with mucicarmine. In the cell block examination, in cases with sufficient cell counts histopathological diagnosis was performed. Results: Of the total undergoing conventional cytological analyses, 154 (79.4%)were reported as benign, 33 (17%) as malignant and 7 (3.6%) as suspicious of malignancy. With the cell block method the results were 147 (75.8%) benign, 12 (6.2%) metastatic, 4 (2.1%) squamous cell carcinoma, 18 (9.3%) adenocarcinoma, 5 (2.6%) large cell carcinoma, 2 (1%) mesothelioma, 3 (1.5%) small cell carcinoma, and 3 (1.5%) lymphoma. Conclusions: Our study confirmed that the cell block method increases the diagnostic yield with exudative pleural effusions accompanying lung cancer.
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문제 정의
, 2012). In this study we aimed to compare cell block technique with the conventional techniques and emphasize the importance of it.
In this study, evaluating the program of cell block preparation with increased cytological diagnosis sensitivity, identifying malignant effusions and demonstrating the superiority of cell block technique compared to conventional technique in identifying the primary region are aimed.
제안 방법
This study includes 194 patients clinically and radiologicaly proven to have pleural effusion in Konya Education and Research Hospital between 2008 and 2013. The fluids gathered from the patients were divided to two equal parts, while in one half conventional cytological analysis was done, in the other half the analysis was done with the cell block technique. Half of the specimens were centrifuged for 5 minutes with 2000 rpms.
대상 데이터
1 with stomach carcinoma and 1 carcinoma metastasis were detected as well. The 194 patients included to the study were 57.2% (111) men and 42.8% (83) were women. 12 (0.
This study includes 194 patients clinically and radiologicaly proven to have pleural effusion in Konya Education and Research Hospital between 2008 and 2013. The fluids gathered from the patients were divided to two equal parts, while in one half conventional cytological analysis was done, in the other half the analysis was done with the cell block technique.
성능/효과
8%) were affirmed benign with the conventional technique was detected malignant with cell block technique. Besides, the 2 (0.6%) of the 31 cases affirmed malignant with the conventional technique, were detected benign with the cell block technique and also the 3 (42%) of the 7 cases affirmed suspiciously malignant with the conventional technique was determined as benign with the cell block technique (Table 3). In our study, the specificity of conventional cytology method is 96%, the sensitivity is 50%.
, 2013). Correspondingly, in our study, 12 cases considered benign with Conventional smear cytology technique was found out that they were actually malignant with the cell block technique and again with the cell block technique adenocarcinoma was the most common diagnosis with 18 cases among all of the malignant cases.
In conclusion, in patients with lung cancer accompanied with pleural effusion, the fluid obtained from thoracentesis, should be examined with both the Conventional smear cytology and cell block technique cytologically, in order to make the benign or malignant discrimination and increase the right diagnosis rate. It is crucial to popularize the usage of the cell block technique, which is very important in making a cancer diagnosis in patients with pleural effusion.
6%) suspiciously malignant (Table-1). The group where the examination was done with cell block method the results were: 147 (75.8%) benign, 12 (6.2%) metastasis, 4 (2.1%) squamous cell carcinoma, 18 (9.3%) adeno carcinoma, 5 (2.6%) large cell carcinoma, 2 (1%) mesothelioma, 3 (1.5%) small cell carcinoma, 3 (1.5%) lymphoma (Table 2). Breast cancer, with 8 patients, was the most common in the metastasis group the second was over carcinoma metastasis with 2 patients.
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