To investigate the memory-enhancing effect of lactic acid bacteria, we selected the probiotic mixture KF, which consisted of Lactobacillus plantarum KY1032 and Lactobacillus curvatus HY7601 (1 × 1011 CFU/g of each strain), and investigated its antilipidemic and memoryenhancing effects in aged Fische...
To investigate the memory-enhancing effect of lactic acid bacteria, we selected the probiotic mixture KF, which consisted of Lactobacillus plantarum KY1032 and Lactobacillus curvatus HY7601 (1 × 1011 CFU/g of each strain), and investigated its antilipidemic and memoryenhancing effects in aged Fischer 344 rats. KF (1 × 1010 CFU/rat/day), which was administered orally once a day (6 days per week) for 8 weeks, significantly inhibited age-dependent increases of blood triglyceride and reductions of HDL cholesterol (p < 0.05). KF restored agereduced spontaneous alternation in the Y-maze task to 94.4% of that seen in young rats (p < 0.05). KF treatment slightly, but not significantly, shortened the escape latency daily for 4 days. Oral administration of KF restored age-suppressed doublecortin and brain-derived neurotrophic factor expression in aged rats. Orally administered KF suppressed the expression of p16, p53, and cyclooxygenase-2, the phosphorylation of Akt and mTOR, and the activation of NF-κB in the hippocampus of the brain. These findings suggest that KF may ameliorate age-dependent memory deficit and lipidemia by inhibiting NF-κB activation.
To investigate the memory-enhancing effect of lactic acid bacteria, we selected the probiotic mixture KF, which consisted of Lactobacillus plantarum KY1032 and Lactobacillus curvatus HY7601 (1 × 1011 CFU/g of each strain), and investigated its antilipidemic and memoryenhancing effects in aged Fischer 344 rats. KF (1 × 1010 CFU/rat/day), which was administered orally once a day (6 days per week) for 8 weeks, significantly inhibited age-dependent increases of blood triglyceride and reductions of HDL cholesterol (p < 0.05). KF restored agereduced spontaneous alternation in the Y-maze task to 94.4% of that seen in young rats (p < 0.05). KF treatment slightly, but not significantly, shortened the escape latency daily for 4 days. Oral administration of KF restored age-suppressed doublecortin and brain-derived neurotrophic factor expression in aged rats. Orally administered KF suppressed the expression of p16, p53, and cyclooxygenase-2, the phosphorylation of Akt and mTOR, and the activation of NF-κB in the hippocampus of the brain. These findings suggest that KF may ameliorate age-dependent memory deficit and lipidemia by inhibiting NF-κB activation.
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제안 방법
For the measurement of memory-related behavioral responses, the Y-maze task was carried out, as previously reported [20], 1 h after the final oral administration of KF (1×1010 CFU/rat) or vehicle (GSB). The Morris water maze task was carried out for 4 days as previously reported [20], 1h after oral administration of KF (1 × 1010 CFU/rat) or vehicle on each consecutive day.
CFU/rat) or vehicle (GSB). The Morris water maze task was carried out for 4 days as previously reported [20], 1h after oral administration of KF (1 × 1010 CFU/rat) or vehicle on each consecutive day.
To identify the potential inhibitory effect of KF on age dependent lipidemia, we measured the blood TG, total cholesterol, and HDL cholesterol levels in young and aged rats (Fig. 1). Although aging slightly, but not significantly, increased the body, liver, and epididymal weights, there was a significant increase in blood TG level and decrease in blood HDL cholesterol.
To measure the effect of KF on memory deficit in aged Fischer 344 rats, KF (1 × 1010 CFU/rat/day) or rapamycin (1㎎/㎏/day, used as a positive control) was orally given once a day for 6 days each week; this was continued for 8weeks. Y-maze and Morris water maze tasks were performed sequentially.
데이터처리
All data are presented as the mean ± standard deviation (SD), with statistical significance analyzed by one-way ANOVA and Turkey’s multiple comparison tests (12.0K for Windows; SPSS, Inc., Chicago, IL, USA) (p<0.05).
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