[국내논문]Combined Skin Moisturization of Liposomal Serine Incorporated in Hydrogels Prepared with Carbopol ETD 2020, Rhesperse RM 100 and Hyaluronic Acid원문보기
We investigated the combined moisturizing effect of liposomal serine and a cosmeceutical base selected in this study. Serine is a major amino acid consisting of natural moisturizing factors and keratin, and the hydroxyl group of serine can actively interact with water molecules. Therefore, we hypoth...
We investigated the combined moisturizing effect of liposomal serine and a cosmeceutical base selected in this study. Serine is a major amino acid consisting of natural moisturizing factors and keratin, and the hydroxyl group of serine can actively interact with water molecules. Therefore, we hypothesized that serine efficiently delivered to the stratum corneum (SC) of the skin would enhance the moisturizing capability of the skin. We prepared four different cosmeceutical bases (hydrogel, oil-in-water (O/W) essence, O/W cream, and water-in-oil (W/O) cream); their moisturizing abilities were then assessed using a $Corneometer^{(R)}$. The hydrogel was selected as the optimum base for skin moisturization based on the area under the moisture content change-time curves (AUMCC) values used as a parameter for the water hold capacity of the skin. Liposomal serine prepared by a reverse-phase evaporation method was then incorporated in the hydrogel. The liposomal serine-incorporated hydrogel (serine level=1%) showed an approximately 1.62~1.77 times greater moisturizing effect on the skin than those of hydrogel, hydrogel with serine (1%), and hydrogel with blank liposome. However, the AUMCC values were not dependent on the level of serine in liposomal serine-loaded hydrogels. Together, the delivery of serine to the SC of the skin is a promising strategy for moisturizing the skin. This study is expected to be an important step in developing highly effective moisturizing cosmeceutical products.
We investigated the combined moisturizing effect of liposomal serine and a cosmeceutical base selected in this study. Serine is a major amino acid consisting of natural moisturizing factors and keratin, and the hydroxyl group of serine can actively interact with water molecules. Therefore, we hypothesized that serine efficiently delivered to the stratum corneum (SC) of the skin would enhance the moisturizing capability of the skin. We prepared four different cosmeceutical bases (hydrogel, oil-in-water (O/W) essence, O/W cream, and water-in-oil (W/O) cream); their moisturizing abilities were then assessed using a $Corneometer^{(R)}$. The hydrogel was selected as the optimum base for skin moisturization based on the area under the moisture content change-time curves (AUMCC) values used as a parameter for the water hold capacity of the skin. Liposomal serine prepared by a reverse-phase evaporation method was then incorporated in the hydrogel. The liposomal serine-incorporated hydrogel (serine level=1%) showed an approximately 1.62~1.77 times greater moisturizing effect on the skin than those of hydrogel, hydrogel with serine (1%), and hydrogel with blank liposome. However, the AUMCC values were not dependent on the level of serine in liposomal serine-loaded hydrogels. Together, the delivery of serine to the SC of the skin is a promising strategy for moisturizing the skin. This study is expected to be an important step in developing highly effective moisturizing cosmeceutical products.
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가설 설정
2 and Table 2 clearly demonstrate that the liposomal serine incorporated in the hydrogel in the above study was effective at increasing the moisture content of the skin. The results obtained in this study may support the hypothesis that serine efficiently delivered to the skin enhances the moisture content of the skin. Natural moisturizing factors, including serine, are highly efficient humectants that are able to hold water molecules from the atmosphere and bring them into the SC [14].
Serine is considered to contribute largely to the moisturization of the skin, as hydroxyl groups of serine can actively interact with water molecules [3]. We therefore hypothesized that serine efficiently delivered to the SC of the skin would enhance the moisturizing capability of the skin as demonstrated by the NMF. However, the hydrophilic and low membrane permeable properties of serine were expected to limit its efficacious delivery to the SC and its ability to become available as NMF in the SC.
Natural moisturizing factors, including serine, are highly efficient humectants that are able to hold water molecules from the atmosphere and bring them into the SC [14]. We thus hypothesized that liposomal delivery of serine, the richest component among the hydrophilic amino acids of NMF, could improve the introduction of serine into the SC where NMF is found; as a result, the moisturizing ability of the skin would increase. Furthermore, as the hydrogel employed in this study was useful at moisturizing the skin, the combined effect of liposomal serine and moisturizing bases may be another reason for the effective moisturizing caused by hydrogel incorporated with serine (1%).
제안 방법
In the present study, we aimed to design cosmeceutical formulations with liposomal serine for enhanced delivery of serine to the SC to improve skin moisturization. As cosmeceutical bases play a key role in skin moisturization, four different cosmeceutical bases (hydrogel, oil-in-water (O/W) essence, O/W cream, and water-in-oil (W/O) cream) were prepared, and the moisturizing ability was assessed to select an optimum cosmeceutical base. Thereafter, liposomal serine was eventually incorporated in the selected cosmeceutical base, and the combined skin moisturizing effect of liposomal serine and the base were investigated.
The values were not greatly changed again until the last measurement at 3 h. As the skin moisture content measured in arbitrary units as a function of time did not allow for quantitative comparison of the effect of cosmetic vehicles on skin moisturization, the profiles of the changes in moisture contents of the skin were produced with time (Fig. 1B). The area under the moisture content change-time curves (AUMCC) was used as a parameter for water holding capacity of the skin.
Four different cosmeceutical bases used as liposomal serine carriers and moisturizers were formulated; components and percent compositions of the final formulations tested in the current study are shown in Table 1. In order to compare the effect of cosmeceutical base type on skin moisturization, hydrogel, O/W essence, O/W cream and W/O cream bases were employed. The ingredients listed from Carbopol ETD 2020 to water were in the aqueous phase, while the ingredients listed from Tween 80 to olive oil were in the oil phase.
The measurements were performed in a room in which the temperature and relative humidity were maintained at 21±3oC and 40±5%, respectively.
The measurements were made as a function of time up to 3 hours. Time-dependent changes in skin moisture were then evaluated by calculating the area under the profiles showing changes in skin moistures, with time used to judge the efficiency of skin moisturization [9]. The age of participants ranged from 24∼26, and written informed consent was obtained following a thorough explanation of the study with its purpose and risks.
데이터처리
Student’s t-test was carried out using the Microsoft Excel 2010 program (Microsoft Corporation, WA, USA), and for statistical comparison, p values less than 0.05 were considered statistically significant.
이론/모형
The physical characteristics of liposomal serine such as particle size and zeta potential values were evaluated using a dynamic light scattering method with Zetasizer Nano-ZS (Malvern Instrument, Worcestershire, UK). The stability of the particle sizes of liposomal serine was also examined for 24 h at 37oC.
성능/효과
In conclusion, the increased moisturizing ability of liposomal serine loaded hydrogels was successfully evaluated in a non-concentration dependent manner. Further research should be completed to optimize the levels of liposomal carriers in hydrogel bases with long-term observation.
Unexpectedly, the AUMCC values were not dependent on the level of serine in liposomal serine-loaded hydrogels. Indeed, the maximum AUMCC values were observed with hydrogel with liposomal serine (serine level=1%), and increased serine levels (i.e., 1.5%) caused a decrease in AUMCC values (Table 2).
The AUMCC values of hydrogel with liposomal serine (serine level=1%) were estimated to be 203.21±51.16 and were found to be approximately 1.62∼1.77 times greater than those of hydrogel, hydrogel with serine (1%), and hydrogel with blank liposome (i.e., liposomes without serine).
후속연구
In conclusion, the increased moisturizing ability of liposomal serine loaded hydrogels was successfully evaluated in a non-concentration dependent manner. Further research should be completed to optimize the levels of liposomal carriers in hydrogel bases with long-term observation. This study is expected to be an important step in developing highly effective moisturizing cosmeceutical products.
Further research should be completed to optimize the levels of liposomal carriers in hydrogel bases with long-term observation. This study is expected to be an important step in developing highly effective moisturizing cosmeceutical products.
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