Han, Sang Mi
(Department of Agricultural Biology, National Academy of Agricultural Science)
,
Kim, Jung Min
(Department of Agricultural Biology, National Academy of Agricultural Science)
,
Hong, In Phyo
(Department of Agricultural Biology, National Academy of Agricultural Science)
,
Woo, Soon Ok
(Department of Agricultural Biology, National Academy of Agricultural Science)
,
Kim, Se Gun
(Department of Agricultural Biology, National Academy of Agricultural Science)
,
Jang, Hye Ri
(Department of Agricultural Biology, National Academy of Agricultural Science)
,
Park, Kwan Kyu
(Catholic University of Daegu School of Medicine)
,
Pak, Sok Cheon
(School of Biomedical Sciences, Charles Sturt University)
Royal jelly has been widely used as a health supplement worldwide. However, royal jelly has been implicated in allergic reactions, and we developed a water-soluble royal jelly (WSRJ) without the allergy inducing protein. In this study, we aimed to identify the anti-melanogenic efficacy of WSRJ. B16F...
Royal jelly has been widely used as a health supplement worldwide. However, royal jelly has been implicated in allergic reactions, and we developed a water-soluble royal jelly (WSRJ) without the allergy inducing protein. In this study, we aimed to identify the anti-melanogenic efficacy of WSRJ. B16F1 melanoma cells were first treated with 10 nM α-melanocyte stimulating hormone (α-MSH) and then with various doses of WSRJ. In addition, we investigated the mRNA and protein expression of melanogenesis-related genes such as tyrosinase, tyrosinase related protein-1 (TRP-1) and TRP-2 by reverse transcription-polymerase chain reaction and western blotting. WSRJ directly inhibited tyrosinase and cellular tyrosinase activity, which decreased melanin synthesis in α-MSH stimulated B16F1 melanoma cells a level comparable to that observed with arbutin. WSRJ decreased the mRNA and protein expressions of tyrosinase, TRP-1, and TRP-2, which was comparable to that observed with arbutin. WSRJ has strong anti-melanogenic activity, which invoice direct inhibition of tyrosinase enzyme activity and suppression of expression of melanogenesis related genes. Results from this study suggests that WSRJ is a potential candidate for the treatment of skin pigmentation.
Royal jelly has been widely used as a health supplement worldwide. However, royal jelly has been implicated in allergic reactions, and we developed a water-soluble royal jelly (WSRJ) without the allergy inducing protein. In this study, we aimed to identify the anti-melanogenic efficacy of WSRJ. B16F1 melanoma cells were first treated with 10 nM α-melanocyte stimulating hormone (α-MSH) and then with various doses of WSRJ. In addition, we investigated the mRNA and protein expression of melanogenesis-related genes such as tyrosinase, tyrosinase related protein-1 (TRP-1) and TRP-2 by reverse transcription-polymerase chain reaction and western blotting. WSRJ directly inhibited tyrosinase and cellular tyrosinase activity, which decreased melanin synthesis in α-MSH stimulated B16F1 melanoma cells a level comparable to that observed with arbutin. WSRJ decreased the mRNA and protein expressions of tyrosinase, TRP-1, and TRP-2, which was comparable to that observed with arbutin. WSRJ has strong anti-melanogenic activity, which invoice direct inhibition of tyrosinase enzyme activity and suppression of expression of melanogenesis related genes. Results from this study suggests that WSRJ is a potential candidate for the treatment of skin pigmentation.
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가설 설정
(A) Tyrosinase activity was determined using mu- shroom tyrosinase and by measuring the absorbance of dopaquinone that is produced by an oxidative reaction with mushroom tyrosinase as the enzyme and L-DOPA as the enzyme substrate. (B) Cellular tyrosinase activity was mea- sured using B16F1 melanoma cell lysates. Data are presen- ted as mean±SEM of five independent experiments.
제안 방법
After washing with TBST, proteins were visual- ized using an enhanced chemiluminescence detection sys- tem. Densitometric analysis was performed using Quan- tity One (Bio-Rad, Hercules, USA) to scan the signals.
After washing with TBST, proteins were visual- ized using an enhanced chemiluminescence detection sys- tem. Densitometric analysis was performed using Quan- tity One (Bio-Rad, Hercules, USA) to scan the signals.
We further evaluated the direct inhibitory action of WSRJ on tyrosinase activity by using mushroom tyrosi- nase, and measured the absorbance of dopaquinone which is produced by an oxidative reaction with mushroom ty- rosinase as the enzyme and L-DOPA as the enzyme sub- strate. Fig.
데이터처리
All data are expressed as the mean±standard error of the mean (SEM). Statistical differences among groups were calculated by analysis of variance followed by Dun- can’s multiple range test (SPSS Version 18.0, USA). Dif- ferences with a P value less than 0.
성능/효과
Ar- butin inhibited mRNA expression of melanogenic enzy- mes at levels similar to those of WSRJ. These findings indicate that downregulation of WSRJ on melanogenesis is due to the decreased levels of melanogenic enzymes, tyrosinase, TRP-1 and TRP-2 proteins and mRNA exp- ression in parallel with the inhibition of tyrosinase activ- ity.
Also, WSRJ inhibited the mRNA and pro- tein expression of tyrosinase, TRP-1 and TRP- 2 in α-MSH stimulated B16F1 melanoma cells. These results indicate that WSRJ has anti-melanogenic effects through direct inhibition of tyrosinase activity in parallel with the inhibi- tion of melanogenic enzyme activities in α-MSH stimu- lated B16F1 melanoma cells. To stimulate melanogenesis, we used cAMP inducers such as α-MSH (Imokawa, 2004).
후속연구
This result implies that WSRJ directly inhibits transcription of tyrosinase, TRP-1, and TRP-2 to produce the inhibition of melanin synthesis in B16F1 melanoma cells. Further studies are necessary to clarify the mechanism of down-regulation. WSRJ is a useful inhibitor of melanogenesis and it might lead to an effective treatment for hyperpigmentation dis- orders.
참고문헌 (23)
1. Ando H. Funasaka Y. Oka M. Ohashi A. Furumura M. Matsunaga J. Matsunaga N. Hearing V. J. Ichihashi M. Possible involvement of proteolytic degradation of tyrosinase in the regulatory effect of fatty acids on melanogenesis J. Lipid Res. (1999) 40 1312 1316 10393216
3. Buscà R. Ballotti R. Cyclic AMP a key messenger in the regulation of skin pigmentation Pigment Cell Res. (2000) 13 60 69 10.1034/j.1600-0749.2000.130203.x 10841026
4. Chang T. S. Chen C. T. Inhibitory effect of homochlorcyclizine on melanogenesis in α-melanocyte stimulating hormone-stimulated mouse B16 melanoma cells Arch. Pharm. Res. (2012) 35 119 127 10.1007/s12272-012-0113-z 22297750
5. Food and Agriculture Organization of the United Nations (FAO) Value-added products from beekeeping (2007) http://www.fao.org/docrep/w0076E/w0076E00.htm
6. Han S. M. Yeo J. H. Cho Y. H. Pak S. C. Royal jelly reduces melanin synthesis through down-regulation of tyrosinase expression Am. J. Chin. Med. (2011) 39 1253 1260 10.1142/S0192415X11009536 22083994
7. Hearing V. J. Biochemical control of melanogenesis and melanosomal organization J. Investig. Dermatol. Symp. Proc. (1999) 4 24 28 10.1038/sj.jidsp.5640176
8. Hearing V. J. Tsukamoto K. Enzymatic control of pigmentation in mammals FASEP J. (1991) 5 2902 2909
9. Imokawa G. Autocrine and paracrine regulation of melanocytes in human skin and in pigmentary disorders Pigment Cell Res. (2004) 17 96 110 10.1111/j.1600-0749.2003.00126.x 15016298
11. Kim J. M. Han S. M. Cho M. R. Lee K. G. Lee M. L. Lee M. Y. Woo S. O. Hong I. P. Sim H. S. Choi Y.S. Characterization of water soluble royal jelly removed allergenic protein Korean J. Apiculture (2013) 28 19 23
12. Kohno K. Okamoto I. Sano O. Arai N. Iwaki K. Ikeda M. Kurimoto M. Royal jelly inhibits the production of proinflammatory cytokines by activated macrophages Biosci. Biotechnol. Biochem. (2004) 68 138 145 10.1271/bbb.68.138 14745176
13. Koya-Miyata S. Okamoto I. Ushio S. Iwaki K. Ikeda M. Kurimoto M. Identification of a collagen production-promoting factor from an extract of royal jelly and its possible mechanism Biosci. Biotechnol. Biochem. (2004) 68 767 773 10.1271/bbb.68.767 15118301
14. Lee E. H. Lim Y. J. Ha S. K. Kang T. H. Koketsu M. Kang C. Kim S. Y. Park J. H. Inhibitory effects of 5-chloroacetyl-2-piperidino-1,3-selenazole, a novel selenium-containing compound, on skin melanin biosynthesis J. Pharm. Pharmacol. (2010) 62 352 359 10.1211/jpp.62.03.0010 20487219
15. Lercker G. Capella P. Conte L. S. Ruini F. Giordani G. Components of royal jelly II. The lipid fraction, hydrocarbons and sterols J. Apicultural Res. (1982) 21 178 184
16. Park H. M. Cho M. H. Cho Y. Kim S. Y. Royal jelly increases collagen production in rat skin after ovariectomy J. Med. Food (2012) 15 568 575 10.1089/jmf.2011.1888 22468645
17. Park H. M. Hwang E. S. Lee K. G. Han S. M. Cho Y. H. Kim S. Y. Royal jelly protects against ultraviolet B-induced photoaging in human skin fibroblast via enhancing collagen production J. Med. Food (2011) 14 899 906 10.1089/jmf.2010.1363 21812645
18. Rosmilah M. Shahnaz M. Patel G. Lock J. Rahman D. Masita A. Noormalin A. Characterization of major allergens of royal jelly Apis mellifera Trop. Biomed. (2008) 25 243 251 19287364
19. Solano F. Briganti S. Picardo M. Ghanem G. Hypopigmenting agents: an updated review on biological, chemical and clinical aspects Pigment Cell Res. (2006) 19 550 571 10.1111/j.1600-0749.2006.00334.x 17083484
20. Tolanai S. Morgan J. F. Studies on the in vitro antitumor activity of fatty acids. V. Unsaturated acids Can. J. Biochem. Physiol. (1962) 40 869 875 10.1139/o62-098 14037871
21. Tokunaga K. H. Yoshida C. Suzuki K. M. Maruyama H. Futamura Y. Araki Y. Mishima S. Antihypertensive effect of peptides from royal jelly in spontaneously hypertensive rats Biol. Pharm. Bull. (2004) 27 189 192 10.1248/bpb.27.189 14758031
22. Urabe K. Nakayama J. Hori Y. The pigmentary system: physiology and pathophysiology Oxford University Press New York (1998) 760 766
23. Yokota T. Nishio H. Kubota Y. Mizoguchi M. The inhibitory effect of glabridin from licorice extracts on melanogenesis and inflammation Pigment Cell Res. (1998) 11 355 361 10.1111/j.1600-0749.1998.tb00494.x 9870547
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