[논문]글라이포세이트 노출로 인한 DNA손상에 대한 녹차의 예방적 효과

박정민; 최우익; 진상찬; 이재호; 최인장

doi:10.22537/jksct.14.2.78

글라이포세이트 노출로 인한 DNA손상에 대한 녹차의 예방적 효과
In vitro Effects of Epigallocatechin Gallate on Sister Chromatid Exchange in the Lymphocytes Exposed to Glyphosate 원문보기

대한임상독성학회지 = Journal of the Korean society of clinical toxicology, v.14 no.2, 2016년, pp.78 - 82

박정민 (계명대학교 의과대학 동산의료원 응급의학교실) , 최우익 (계명대학교 의과대학 동산의료원 응급의학교실) , 진상찬 (계명대학교 의과대학 동산의료원 응급의학교실) , 이재호 (계명대학교 의과대학 해부학교실) , 최인장 (계명대학교 의과대학 해부학교실)

Abstract ▼ AI-Helper

Purpose: Green tea is known as a potent anti-oxidant, anti-carcinogen, and genetic protector. Glyphosate (N-phosphonomethyl glycine) is a widely used non-selective herbicide that causes DNA damage. The present study was conducted to investigate the protective effects of green tea in human blood lymphocytes exposed to glyphosate using the Sister Chromatid Exchange (SCE) frequency method. Methods: Peripheral blood was obtained from 10 volunteers and cultured through four different conditions. Four groups were divided into control, glyphosate only (300 ng/mL), glyphosate and low ($20{\mu}m$) concentrations of epigallocatechin gallate (EGCG) and glyphosate and high ($100{\mu}m$) concentrations of EGCG. Results: The glyphosate exposed groups had a higher mean SCE frequency ($10.33{\pm}2.50$) than the control group ($6.38{\pm}2.28$, p<0.001). The low concentrations of EGCG groups had a lower mean SCE frequency ($9.91{\pm}1.93$) than the glyphosate-only group, although this difference was not significant (p=0.219). However, the high concentration group ($9.49{\pm}1.85$) had a significantly lower SCE frequency than the glyphosate-only group (p=0.001). Conclusion: EGCG has a gene protective effect in human lymphocytes exposed to the genotoxicity of glyphosate in the case of high concentrations.

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문제 정의

The aim of this study was to know protective effect of EGCG in human blood lymphocyte exposed to genotoxicity of glyphosate by SCE method. GT is mainly comprised of EGCG, ECG, EGC and EC, therefore, their anti-genotoxic effects against glyphosate were analyzed in 3 subjects preliminarily.
It is difficult to know the detail mechanisms of both ‘genotoxic effect of glyphosate’ and ‘genetic protective effect of EGCG’. This study was done to clarify protective effect of EGCG in human blood lymphocyte exposed to genotoxicity of glyphosate by SCE frequency method.

제안 방법

All of blood samples were experimented together through four groups that divided by concentrations of glyphosate and EGCG. Group 1 is control group that contains no glyphosate and no EGCG.
Of the catechins, polyphenolic components of GT, EGCG is the major constituent and also most active component with the highest antioxidant properties²³⁾. Therefore, our further main experiment was performed by EGCG and its anti-genotoxic effect against glyphosate was evaluated.

대상 데이터

The frequency of SCE was examined in the 10 healthy-male volunteers. The mean and standard deviation (SD) of each group were calculated and summarized in Table 1.

데이터처리

In this experiment, the statistical comparisons of the mean number of SCEs from each group were performed using the one-way ANOVA method. The TUKEY post-testing was utilized for multiple comparisons.

이론/모형

1 ml (10 μg/mL) of colcemid (Gibco, Uxbridge, UK) was added to arrest mitosis at metaphase. All the chromosome preparations were stained using the BrdU-Hoechst-Giemsa technique. The SCE of the lymphocytes was microscopically examined and counted using the Cytovision Computer-Assisted Karyotyping System (Applied Imaging, Santa Clara, CA, USA).

성능/효과

Also, this study showed EGCG has a protective effect against genotoxicity of glyphosate in case of only high concentration (100 μM) not low concentration (20 μM).
GT is mainly comprised of EGCG, ECG, EGC and EC, therefore, their anti-genotoxic effects against glyphosate were analyzed in 3 subjects preliminarily. As a result, EGCG showed a lowest SCE frequency in the cytotoxicity by glyphosate compared to ECG, EGC and EC, though it did not have statistical significance. Of the catechins, polyphenolic components of GT, EGCG is the major constituent and also most active component with the highest antioxidant properties²³⁾.

후속연구

Accordingly, the experiments in the lymphocyte of its acute exposure patient and in vivo studies might figure out its cytotoxicity. Therefore, further study with clinically available dose should be performed. And the effect of other catechins in GT also should be studied in various subject based on various kinds of personal consequences and side effects of EGCG.

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연합인증

글라이포세이트 노출로 인한 DNA손상에 대한 녹차의 예방적 효과
In vitro Effects of Epigallocatechin Gallate on Sister Chromatid Exchange in the Lymphocytes Exposed to Glyphosate 원문보기

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글라이포세이트 노출로 인한 DNA손상에 대한 녹차의 예방적 효과 In vitro Effects of Epigallocatechin Gallate on Sister Chromatid Exchange in the Lymphocytes Exposed to Glyphosate 원문보기

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글라이포세이트 노출로 인한 DNA손상에 대한 녹차의 예방적 효과
In vitro Effects of Epigallocatechin Gallate on Sister Chromatid Exchange in the Lymphocytes Exposed to Glyphosate 원문보기

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