황금추출물이 과산화지질을 급여한 흰쥐의 지질대사, 산화반응 및 전염증성 Cytokine의 생산에 미치는 영향 Effects of Scutellariae Radix Extract on Lipid Metabolism, Oxidation and Production of Pro-Inflammatory Cytokines in Rats Fed Highly Oxidized Fat원문보기
목적: 본 연구는 황금추출물이 과산화지질을 장기간 급여한 흰쥐의 지질대사, 산화반응 및 전염증성 cytokine의 생산에 미치는 영향을 검토하기 위해 수행되었다. 방법: 10주 동안 흰쥐에게 과산화지질을 과량으로 급여하여 생체 내에 과잉의 지질을 축적시킨 후, 황금추출물을 6주간 급여하였다. 그 후, 각 처리군별로 지질대사, 산화반응 및 전염성 cytokine의 생산에 미치는 영향을 조사했다. 결과: 혈액 내 유리지방산, 총지질, 총콜레스테롤, low density lipoprotein-cholesterol과 간장 내 총지질, 총콜레스테롤이 황금추출물 처리군에서 하락했다. 혈액 및 간장의 thiobarbituric acid 농도가 황금추출물 처리군에서 하락했다. 간장 내 항산화효소들(glutathione peroxidase, liver catalase, liver superoxide dismutase)의 활성치가 황금처리군에서 상승하였다. 전염증성 cytokine(interleukin $[IL]-1{\beta}$, tumor necrosis $factor-{\alpha}$, IL-6)의 농도는 황금추출물 처리군에서 하락했다. 반면에 IL-10의 농도는 황금추출물 처리군에서 증가했다. 결론: 이상의 결과들을 종합해 보면, 황금 추출물은 과산화지질을 급여한 비만쥐의 지질대사, 항산화반응 및 항염증반응에 긍정적인 효과를 나타내었음을 인식시켜 준다.
목적: 본 연구는 황금추출물이 과산화지질을 장기간 급여한 흰쥐의 지질대사, 산화반응 및 전염증성 cytokine의 생산에 미치는 영향을 검토하기 위해 수행되었다. 방법: 10주 동안 흰쥐에게 과산화지질을 과량으로 급여하여 생체 내에 과잉의 지질을 축적시킨 후, 황금추출물을 6주간 급여하였다. 그 후, 각 처리군별로 지질대사, 산화반응 및 전염성 cytokine의 생산에 미치는 영향을 조사했다. 결과: 혈액 내 유리지방산, 총지질, 총콜레스테롤, low density lipoprotein-cholesterol과 간장 내 총지질, 총콜레스테롤이 황금추출물 처리군에서 하락했다. 혈액 및 간장의 thiobarbituric acid 농도가 황금추출물 처리군에서 하락했다. 간장 내 항산화효소들(glutathione peroxidase, liver catalase, liver superoxide dismutase)의 활성치가 황금처리군에서 상승하였다. 전염증성 cytokine(interleukin $[IL]-1{\beta}$, tumor necrosis $factor-{\alpha}$, IL-6)의 농도는 황금추출물 처리군에서 하락했다. 반면에 IL-10의 농도는 황금추출물 처리군에서 증가했다. 결론: 이상의 결과들을 종합해 보면, 황금 추출물은 과산화지질을 급여한 비만쥐의 지질대사, 항산화반응 및 항염증반응에 긍정적인 효과를 나타내었음을 인식시켜 준다.
Objectives: This study investigated the effects of Scutellariae Radix extract (SRE) on lipids metabolism, oxidation and the production of pro-inflammatory cytokines in rats fed highly oxidized fat. Methods: To induce obesity, male Sprague‐Dawley rats were fed a highly oxidized fat diet for 10 weeks....
Objectives: This study investigated the effects of Scutellariae Radix extract (SRE) on lipids metabolism, oxidation and the production of pro-inflammatory cytokines in rats fed highly oxidized fat. Methods: To induce obesity, male Sprague‐Dawley rats were fed a highly oxidized fat diet for 10 weeks. SRE at 100 mg/kg were administered orally to obesity-induced rats for 6 weeks, and their lipid metabolism, oxidation and production of pro-inflammatory cytokines were examined. Results: The concentrations of free fatty acid, triglyceride, total cholesterol, and low density lipoprotein-cholesterol in plasma decreased in SRE-treated groups, although the difference was not significant between control and SRE-treated groups, while that of high density lipoprotein-cholesterol significantly increased in SRE group. The concentrations of total cholesterol and triglyceride in the liver were tended to decrease in SRE-treated group. The concentrations of thiobarbituric acid in plasma and liver were lower in SRE group than in control group. The levels of glutamic oxaloacetic transaminase and glutamic pyruvic transaminase in plasma were decreased in SRE group. Activities of glutathione peroxidase, superoxide dismutase, and catalase in liver were tended to increase in the SRE group. The plasma concentrations of interleukin $(IL)-1{\beta}$, tumor necrosis factor $(TNF)-{\alpha}$ and IL-6 were lower in SRE group than in control group, while that of IL-10 was higher. The liver concentrations of $IL-1{\beta}$, $TNF-{\alpha}$, and IL-6 were tended to decrease while that of IL-10 tended to increase in SRE group. Conclusions: Finally SRE could be used in the production of nutraceuticals for lowering lipids and exerting anti-oxidation and anti-inflammatory effects in obesity rats fed highly oxidized rat.
Objectives: This study investigated the effects of Scutellariae Radix extract (SRE) on lipids metabolism, oxidation and the production of pro-inflammatory cytokines in rats fed highly oxidized fat. Methods: To induce obesity, male Sprague‐Dawley rats were fed a highly oxidized fat diet for 10 weeks. SRE at 100 mg/kg were administered orally to obesity-induced rats for 6 weeks, and their lipid metabolism, oxidation and production of pro-inflammatory cytokines were examined. Results: The concentrations of free fatty acid, triglyceride, total cholesterol, and low density lipoprotein-cholesterol in plasma decreased in SRE-treated groups, although the difference was not significant between control and SRE-treated groups, while that of high density lipoprotein-cholesterol significantly increased in SRE group. The concentrations of total cholesterol and triglyceride in the liver were tended to decrease in SRE-treated group. The concentrations of thiobarbituric acid in plasma and liver were lower in SRE group than in control group. The levels of glutamic oxaloacetic transaminase and glutamic pyruvic transaminase in plasma were decreased in SRE group. Activities of glutathione peroxidase, superoxide dismutase, and catalase in liver were tended to increase in the SRE group. The plasma concentrations of interleukin $(IL)-1{\beta}$, tumor necrosis factor $(TNF)-{\alpha}$ and IL-6 were lower in SRE group than in control group, while that of IL-10 was higher. The liver concentrations of $IL-1{\beta}$, $TNF-{\alpha}$, and IL-6 were tended to decrease while that of IL-10 tended to increase in SRE group. Conclusions: Finally SRE could be used in the production of nutraceuticals for lowering lipids and exerting anti-oxidation and anti-inflammatory effects in obesity rats fed highly oxidized rat.
* AI 자동 식별 결과로 적합하지 않은 문장이 있을 수 있으니, 이용에 유의하시기 바랍니다.
제안 방법
Intracorporeal peroxide accumulation is closely interrelated with the activities of antioxidant enzymes. In this study, the activities of GSH-Px, SOD, and CAT corresponded to the changes in TBARS concentrations. These results suggest that functional substances in SRE act on the activities of antioxidant enzymes and the biologic responses in the production inhibition and lysis promotion of intracorporeal peroxide.
. With the aim of developing nutraceuticals for lowering lipids and with antioxidant and anti-inflammatory effects, this study investigated the effects of SRE on lipids, anti-oxidation, and the concentration of pro-inflammatory cytokines in rats fed highly oxidized fat.
데이터처리
Statistical analyses (mean±SD) were performed using one-way ANOVA with the PASW Statistics ver. 18.0Ⓡ for Windows (IBM Co., Armonk, NY, USA), and the cutoff for significance was P＜0.05 by Duncan’s multiple range test.
이론/모형
The plasma thiobarbituric acid (TBARS) concentration was quantified using the method of Buege and Aust23). The hepatic TBARS concentration was measured by the method of Ohkawa et al.24), and glutamic oxaloacetic transaminase (GOT) and glutamic pyruvic transaminase (GPT) activities in plasma were measured using an automatic blood-analyzing device (Boehringer Mannheim, Mannheim, Germany). The activities of glutathione peroxidase (GSH-Px), liver superoxide dismutase (SOD), and liver catalase (CAT) were measured using the methods of Levander et al.
Total plasma concentrations of cholesterol, total hepatic cholesterol, high density lipoprotein (HDL)-cholesterol, and triglyceride were measured using commercial kits (Wako, Japan). The plasma thiobarbituric acid (TBARS) concentration was quantified using the method of Buege and Aust23). The hepatic TBARS concentration was measured by the method of Ohkawa et al.
성능/효과
The concentrations of plasma free fatty acid and triglyceride (Table 2) were lower in the SRE groups than the control group, although the difference was not significant between control and 100 mg/kg SRE. The concentrations of total plasma cholesterol and low density lipoprotein-cholesterol (Table 3) also decreased as the concentration of SRE increased, although the difference was not significant between control and SRE (100 mg/kg)-treated groups.
But in some cases it may differ according to the diet or enzyme-activity-related factors. In this study, since all of the rats had the same intake of the same diet, the results suggest that SRE can influence hepatic lipid synthesis or lipolysis.
참고문헌 (27)
Takayama S, Kahn CR, Kubo K, Foley JE. Alterations in insulin receptor autophosphorylation in insulin resistance: correlation with altered sensitivity to glucose transport and antilipolysis to insulin. J Clin Endocrinol Metab. 1988 ; 66(5) : 992-9.
Robert HE, Ronald MK. American heart association call to action: obesity as a major risk factor for coronary heart disease. Circulation. 1998 ; 97(21) : 2099-100.
Lee E, Choi MY, Oh HS. Effects of Powdered Siho (Bupleuri Radix) on serum and liver lipid composition and antioxidative capacity in rat fed high oxidized fat. Korean J Nutr. 2000 ; 33(5) : 502-6.
Lee E. Effects of powdered pine needle (Pinus densiflora seib et Zucc.) on serum and liver lipid composition and antioxidative. J Korean Soc Food Sci Nutr. 2003 ; 32(6) : 926-30.
Kang YH, Ha TY, Moon KD. Effects of pine needle extracts on serum and liver lipid contents in rats fed high fat diet. J Korean Soc Food Nutr. 1996 ; 25(3) : 367-73.
Ishikawa T, Suzukawa M. Effect of tea flavonoid supplementation on the susceptibility of low-density lipoprotein to oxidative modification. Am J Clin Nutr. 1997 ; 66(2) : 261-6.
Kim HC. Textbook of herbal pharmacology. Seoul : Jipmundang. 2001 : 129-33.
Middleton E Jr, Drzewiecki V. Naturally occuring flavonoids and human basophil histamine release. Int Archs Allergy Appl Immun. 1985 ; 77(1-2) : 155-7.
Wagner H. Antihepatotoxic flavonoids. In: Cody V, editors. Plant flavonoids in biology and medicine, biochemical, pharmacological and structure: activity relationships. New York : Alan R. Liss. 1986 : 545-58.
Middleton E Jr, Harborne JB, Beretz A. Plant flavonoids in biology and medicine II. biochemical, cellular and medicineal properties. New York : Alan R. Liss. 1988 : 61-5.
Middleton E Jr, Harborne JB. Plant flavonoids in biology and medicine, biochemical, pharmacological, structure-activity relationship. New York : Alan R. Liss. 1986 : 429-40.
Kubo MY, Kimura T, Odani T, Tani T, Namba K. Studies on Scutellariae radix. II. The antibacterial substance. Planta Med. 1981 ; 43(2) : 194-201.
Kubo MH, Matusuda Y, Kimura H, Okuda M, Higashino T, Tani T, et al. Stidies on Scutellariae Radix. VII. Anti-arthrictic and anti-inflammatory action of methanolic extract and flavonoid components from Scutellariae Radix. Chem Pharm Bull. 1984 ; 32(7) : 2724-9.
Tie H, Jin GB, Cho GB, Fumi S, Cyong JC. Evaluation of the anti-inflammatory effect of baicalein on dextran sulfate sodium-induced colitis in mice. Planta Med. 2002 ; 68(3) : 266-8.
Levander OA, DeLoach DP, Morris VC, Moser PB. Platelet glutathione peroxidase activity as an index of selenium status in rats. J Nutr. 1983 ; 113(1) : 55-63.
Flohe L, Becker R, Brigelius R, Lengfelder E, Otting F. Convenient assays for superoxide dismutase. CRC Handbook of free radicals and antioxidants in Biomedicine. Boca Raton, FL : CRC Press, Inc. 1992 : 287-93.
Johnson LH, Hakan Borg LA. A spectro phtometric method for determination of catalase activity in small tissue samples. Anal Biochem. 1988 ; 174(1) : 331-6.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.