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Inhibiting the Growth of Escherichia coli O157:H7 in Beef, Pork, and Chicken Meat using a Bacteriophage 원문보기

Korean journal for food science of animal resources = 한국축산식품학회지, v.36 no.2, 2016년, pp.186 - 193  

Seo, Jina (Department of Food and Nutrition, Chung-Ang University) ,  Seo, Dong Joo (Department of Food and Nutrition, Chung-Ang University) ,  Oh, Hyejin (Department of Food and Nutrition, Chung-Ang University) ,  Jeon, Su Been (Department of Food and Nutrition, Chung-Ang University) ,  Oh, Mi-Hwa (National Institute of Animal Science, Rural Development Administration) ,  Choi, Changsun (Department of Food and Nutrition, Chung-Ang University)

Abstract AI-Helper 아이콘AI-Helper

This study aimed to inhibit Escherichia coli (E. coli) O157:H7 artificially contaminated in fresh meat using bacteriophage. Among 14 bacteriophages, the highly lytic bacteriophage BPECO19 strain was selected to inhibit E. coli O157:H7 in artificially contaminated meat samples. Bacteriophage BPECO19 ...

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제안 방법

  • coli O157:H7 were not used in pork and chicken meat. Therefore, the aims of this study were to determine the optimal treatment of bacteriophage to control E. coli O157:H7 and to investigate the inhibi- tion of E. coli O157:H7 inoculated on beef, pork, and chicken by bacteriophage.

대상 데이터

  • E. coli O157:H7 ATCC 43888, E. coli O157:H7 ATCC 43889, and E. coli O157:H7 ATCC 43890 were purchased from American Type Culture Collection (ATCC; USA). Host bacteria were grown in Luria Bertani (LB, Sigma- Aldrich, USA) broth on shaking incubator (SI-600R, Jei- otech, Korea) at 37℃ overnight.
  • Fourteen lytic bacteriophages against E. coli O157:H7 ATCC 43889 were obtained from the bacteriophage bank of Hankuk University of Foreign Studies in Korea (Table 1). The titration of each bacteriophage strain was deter- mined by the plaque assay, as previously described (Hud- son et al.

데이터처리

  • Data from each group were expressed as mean and stan- dard deviation. Statistical significance was analyzed at p< 0.05 by one-way analysis of variance (ANOVA) and paired t-test using Statistical Package for Social Science (SPSS) software v18 (IBM, USA).
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참고문헌 (30)

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