Lee, Kyung Hee
(Department of Food and Nutrition, Hanyang University)
,
Bae, In Young
(Department of Food and Fermentation, Far East University)
,
Park, Song I.
(Department of Food and Nutrition, Hanyang University)
,
Park, Jong-Dae
(Department of Food and Nutrition, Hanyang University)
,
Lee, Hyeon Gyu
(Department of Food and Nutrition, Hanyang University)
Background: Ginsenoside Rg3 and arginine-fructose (Arg-Fru) are known as the hypotensive compounds of Panax ginseng; however, their efficacy on antihypertension has not been reported yet to our best knowledge. Thus, hypotensive components-enriched fraction of red ginseng (HCEF-RG) was prepared from ...
Background: Ginsenoside Rg3 and arginine-fructose (Arg-Fru) are known as the hypotensive compounds of Panax ginseng; however, their efficacy on antihypertension has not been reported yet to our best knowledge. Thus, hypotensive components-enriched fraction of red ginseng (HCEF-RG) was prepared from fine root concentrate (FR) and their antihypertensive effects were investigated in spontaneously hypertensive rats (SHR). Methods: Male SHRs were divided into six groups: control (Wistar Kyoto, SHR); FR 500; FR 1,000; HCEF-RG 500; and HCEF-RG 1,000; samples (mg/kg body weight) were orally administered every day for 8 wk. Blood pressure was monitored at 1 wk, 2 wk, 3 wk, 4 wk, 6 wk, and 8 wk by tail cuff method. At 8 wk after samples administration, mice were killed for the measurement of renin activity (RA), angiotensin-I converting enzyme inhibition, angiotensin II, and nitric oxide (NO) levels in plasma. Results: HCEF-RG with four-fold more Rg3 and 24-fold more Arg-Fru contents was successfully prepared from reacted mixtures of FR and persimmon vinegar (12 times against FR, v/v) at $80^{\circ}C$ for 18 h. Both FR 1,000 and HCEF-RG 1,000 showed lowered systolic blood pressure than SHR control group and HCEF-RG 1,000 group exhibited a significant decrease in diastolic blood pressure. RA was significantly lowered in all treated groups, while angiotensin II did not affect by FR and HCEF-RG treatment. However, angiotensin-I converting enzyme inhibition and NO in FR 1,000 and HCEF-RG 1,000 were significantly increased compared with SHR control group. Conclusion: HCEF-RG is more effective and useful for alleviating hypertension than FR, implying the health benefit of Rg3 and Arg-Fru.
Background: Ginsenoside Rg3 and arginine-fructose (Arg-Fru) are known as the hypotensive compounds of Panax ginseng; however, their efficacy on antihypertension has not been reported yet to our best knowledge. Thus, hypotensive components-enriched fraction of red ginseng (HCEF-RG) was prepared from fine root concentrate (FR) and their antihypertensive effects were investigated in spontaneously hypertensive rats (SHR). Methods: Male SHRs were divided into six groups: control (Wistar Kyoto, SHR); FR 500; FR 1,000; HCEF-RG 500; and HCEF-RG 1,000; samples (mg/kg body weight) were orally administered every day for 8 wk. Blood pressure was monitored at 1 wk, 2 wk, 3 wk, 4 wk, 6 wk, and 8 wk by tail cuff method. At 8 wk after samples administration, mice were killed for the measurement of renin activity (RA), angiotensin-I converting enzyme inhibition, angiotensin II, and nitric oxide (NO) levels in plasma. Results: HCEF-RG with four-fold more Rg3 and 24-fold more Arg-Fru contents was successfully prepared from reacted mixtures of FR and persimmon vinegar (12 times against FR, v/v) at $80^{\circ}C$ for 18 h. Both FR 1,000 and HCEF-RG 1,000 showed lowered systolic blood pressure than SHR control group and HCEF-RG 1,000 group exhibited a significant decrease in diastolic blood pressure. RA was significantly lowered in all treated groups, while angiotensin II did not affect by FR and HCEF-RG treatment. However, angiotensin-I converting enzyme inhibition and NO in FR 1,000 and HCEF-RG 1,000 were significantly increased compared with SHR control group. Conclusion: HCEF-RG is more effective and useful for alleviating hypertension than FR, implying the health benefit of Rg3 and Arg-Fru.
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제안 방법
, Seoul, Korea). The animals were allowed to acclimate for a wk and then randomly divided into six groups (n = 8 each): WKY-control; SHR-control; SHR- FR 500; SHR- FR 1,000; SHR- HCEF-RG 500; and SHR- HCEF-RG 1,000. The animals were housed individually in stainless steel cages arranged in a randomized complete block design at a temperature of 23 1°C and humidity of 53 ± 2% in a light-controlled room under a 12 h light-dark cycle.
The animals were housed individually in stainless steel cages arranged in a randomized complete block design at a temperature of 23 1°C and humidity of 53 ± 2% in a light-controlled room under a 12 h light-dark cycle.
Therefore, if ginsenoside Rg3-Arg-Fru-enrichment materials can be produced, they will be useful in alleviating hypertension and consequently allowing the food industry to develop new hypotensive compounds-fortified products. Thus, in this study, we prepared a hypotensive components-enriched fraction from red ginseng (HCEF-RG) under various conditions with persimmon vinegar and evaluated the ability of HCEF-RG to improve the hypertension in spontaneously hypertensive rats (SHR).
대상 데이터
The experiment was performed using 8-wk-old male Wistar Kyoto rats (WKY; normal group, male, 130-175 g body weight; Charles River Co., Kanawa, Japan) and SHR (hypertension group, male, 180-215 g body weight, Orient Bio Co. Ltd., Seoul, Korea). The animals were allowed to acclimate for a wk and then randomly divided into six groups (n = 8 each): WKY-control; SHR-control; SHR- FR 500; SHR- FR 1,000; SHR- HCEF-RG 500; and SHR- HCEF-RG 1,000.
데이터처리
1) Values with different superscripts in the same bar are significantly different among samples at a = 0.05 level by Duncan’s multiple range test.
1) Values with different superscripts within the same column are significantly different among samples at a = 0.05 level by Duncan’s multiple range test.
The significance of differences (p < 0.05) among the corresponding mean values was determined using one-way analysis of variance (ANOVA) followed by Duncan’s multiple comparison test or Student t test (SPSS, version 17.0; SPSS Inc., Chicago, IL, USA).
Values with different superscripts in the same bar are significantly different among samples at a = 0.05 level by Duncan’s multiple range test.
이론/모형
After stabilization for 15 min at 37°C in a mold, blood pressure was measured by the tail cuff method.
성능/효과
After 8 wk of the study, the rats fed FR or HCEF-RG at 1,000 mg/kg body weight had significantly (p < 0.05) lower systolic blood pressure than the control group.
In conclusion, HCEF-RG was successfully prepared by persimmon vinegar treatment (12 times against fine root concentrates, v/v) at 80oC for 18 h. Systolic blood pressure and diastolic blood pressure in the SHR groups treated with HCEF-RG decreased significantly, compared with the SHR control group (p < 0.
Rg3 has been shown to inhibit the activation of ACE, thus contributing to the prevention of hypertension [25]. The Rg3 content in FR and HCEF-RG and the concentrationdependent effects of ACE inhibition were confirmed in this study; Rg3 enrichment further promoted ACE inhibition. In the case of the HCEF-RG 1,000 mg/kg group, which showed a 29% ACE inhibiting activity compared to the SHR control, a 16% inhibiting activity was observed for angiotensin II.
The results indicate that the magnitude of the diastolic blood pressure response of the HCEF-RG 1,000 mg/kg body weight group was significantly (p < 0.05) decreased compared to the SHR control group after 8 wk of study.
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