Kwak, Kyu-Won
(Department of Agricultural Biology, National Institute of Agricultural Science)
,
Han, Myung-Sae
(Department of Biofibers and Material Science, Kyungpook National University)
,
Nam, Sung-Hee
(Department of Agricultural Biology, National Institute of Agricultural Science)
,
Park, Kwan-Ho
(Department of Agricultural Biology, National Institute of Agricultural Science)
,
Kim, Eun-Sun
(Department of Agricultural Biology, National Institute of Agricultural Science)
,
Lee, Seokhyun
(Department of Agricultural Biology, National Institute of Agricultural Science)
,
Song, Myung-Ha
(Department of Agricultural Biology, National Institute of Agricultural Science)
,
Kim, Wontae
(Department of Agricultural Biology, National Institute of Agricultural Science)
,
Choi, Ji-Young
(Department of Agricultural Biology, National Institute of Agricultural Science)
Beauveria bassiana is a common fungal pathogen of Protaetia brevitarsis larvae, and although it is less common than Metarhizium anisopliae , the pathogen still poses a great risk to humans and animals that consume infected insects, owing to B. bassiana's production of toxins like beauvericin and myc...
Beauveria bassiana is a common fungal pathogen of Protaetia brevitarsis larvae, and although it is less common than Metarhizium anisopliae , the pathogen still poses a great risk to humans and animals that consume infected insects, owing to B. bassiana's production of toxins like beauvericin and mycotoxin. Interestingly, the beneficial microorganism Saccharomyces cerevisiae possesses antifungal properties. In the present study, we found that S. cerevisiae inhibited the growth of B. bassiana by 97% and that S. cerevisiae failed to harm P. brevitarsis when administered via intracoelomic injection (1×107 cfu/mL). In addition, we also found that S. cerevisiae consumption increased the survival time of percutaneously infected P. brevitarsis larvae by 5 d and reduced the mortality of infected larvae by 12%. Therefore, S. cerevisiae is expected to be useful in the prevention and control of B. bassiana in the production of P. brevitarsis larvae.
Beauveria bassiana is a common fungal pathogen of Protaetia brevitarsis larvae, and although it is less common than Metarhizium anisopliae , the pathogen still poses a great risk to humans and animals that consume infected insects, owing to B. bassiana's production of toxins like beauvericin and mycotoxin. Interestingly, the beneficial microorganism Saccharomyces cerevisiae possesses antifungal properties. In the present study, we found that S. cerevisiae inhibited the growth of B. bassiana by 97% and that S. cerevisiae failed to harm P. brevitarsis when administered via intracoelomic injection (1×107 cfu/mL). In addition, we also found that S. cerevisiae consumption increased the survival time of percutaneously infected P. brevitarsis larvae by 5 d and reduced the mortality of infected larvae by 12%. Therefore, S. cerevisiae is expected to be useful in the prevention and control of B. bassiana in the production of P. brevitarsis larvae.
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대상 데이터
Bacillus subtilis (KACC17047), Lactobacillus plantarum (KACC10552), and Saccharomyces cerevisiae (KACC30068) were obtained from the KACC and cultured in liquid MRS, R2A, and YEPD media, respectively, at 30 ℃. Subsequently, 100 μL S.
cerevisiae (1 × 107 cfu/50 µL); triple-distilled water, as a negative control; or YPD broth, as an another positive control, using a BD ultra- fine insulin syringe (31 gauge, 6 mm). Ten larvae were included in each group, and each treatment was performed in triplicate. After injection, specimens were fed with fermented sawdust, and mortality was assessed at 7 d after injection.
The larvae were inoculated twice, with a 2-h interval, and the larvae were also inoculated a third time at 24 h after the second inoculation. The experiment was performed in triplicate, and 10 larvae were used for each repetition. Following inoculation with B.
The mortality of the larvae was measured after 10 d. The experiment was performed in triplicate.
데이터처리
The statistical analysis was conducted using SPSS (Statistical Package for the Social Sciences, USA). The B. bassiana mycelia growth was analyzed by ANOVA (analysis of variance).
이론/모형
bassiana. At 30 d after seeding with B. bassiana, the radius of the fungal colonies were measured, and we calculated the percent growth inhibition according to the agar well diffusion assay method described by Grover and Moore (1962), using the following equation: MGI (Inhibition of mycelial growth) = (DC-DT)/DC × 100%, DC = diameter of control, DT = diameter of test (Padency et al., 1982). The statistical analysis was conducted using SPSS (Statistical Package for the Social Sciences, USA).
성능/효과
In conclusion, the present study demonstrated that S. cerevisiae, L. plantarum, and B. subtilis inhibited the in vitro growth of B. bassiana; that S. cerevisiae had no harmful effect when injected into the hemolymph of P. brevitarsis larvae; and that a S. cerevisiae-inoculated food source delayed B. bassiana infection and reduced P. brevitarsis mortality in vivo. No previous studies have reported the pathogenic potential of S.
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