[논문]TRAIL과 방사선 조사가 암세포의 사멸에 미치는 효과

이재섭; 장성주

doi:10.7742/jksr.2016.10.6.387

초록
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종양을 효율적으로 적출, 제거하기 위해서는 부수적으로 방사선 치료 및 항암화학요법을 이용하여 수술 전이나, 수술 후 종양의 크기를 줄이거나 작게 할 수는 있었으나, 종양을 적출하거나 제거하는 방법으로 외과적 절제수술요법이 가장 재래적인 수술 방법이다. 종양의 크기를 줄이거나 작게 하는 방법과, 종양을 사멸시킬 수 있는 항암화학요법은 방사성 감수성을 증가시키는 항암약제를 종양세포에 선택적으로 투여하여 방사선에 대한 감수성에 민감하게 반응 할 수 있도록 한 것이며, 다양한 생물학적인 세포증식억제 방법 중 TRAIL은 단백질을 변형 시킬 수 있으며 단백질 구조를 변형시켜 세포의 사멸에 일조를 하는 것으로 알려져 있다. 본 논문에서는 HCT-116세포를 암세포로 간주하여 TRAIL과 방사선과의 상호관계를 분석하였다. 실험결과 TRAIL과 방사선의 단독사용은 대조군과 비교해 본 결과 각각 세포증식과 세포자멸사에 유의적인 영향을 끼치지 않은 것으로 나타났다. 반대로 TRAIL로 처리하고, 방사선 조사를 병행해서 처리한 경우 HCT-116세포가 유의적으로 세포자멸사가 발생되었음을 알 수 있었고, G1대비 G0의 비율도 증가한 것으로 나타났다. 결론적으로 TRAIL은 방사선 방어적인 세포의 세포자멸사를 증가시켜 방사선 감수성을 증가시켰음을 알 수 있었으며, 또한 세포주기를 변화시켜 세포 증식 능력을 점진적으로 감소시킬 수 있었다. TRAIL은 세포자멸사를 증가시키고 세포증식 능력을 감소시켜 방사선 증감제로서 사용이 가능하다는 것으로 사료된다.

Abstract ▼ AI-Helper

Tumor using the efficient concomitant radiotherapy and chemotherapy to remove, prior to surgery and, either reduce the size of the tumor after surgery, or was can be made smaller, Or excised tumor, in a way to be removed, most conventional surgical method is surgical excision surgery therapy. And me...

Tumor using the efficient concomitant radiotherapy and chemotherapy to remove, prior to surgery and, either reduce the size of the tumor after surgery, or was can be made smaller, Or excised tumor, in a way to be removed, most conventional surgical method is surgical excision surgery therapy. And methods reduce or tumor size, or smaller, chemotherapy can kill tumor is administered selectively anticancer agent which increases the radioactive susceptible to tumor cells, sensitive to susceptibility to radiation are those which make it possible to respond to, either TRAIL methods of various biological cytostatic can deform the protein, by deforming the structure of the protein help to cell death it is known. In this paper, the HCT-116 cells thought to be a cancer cell to analyze the interaction of TRAIL and radiation. Experimental results, single use of TRAIL and radiation, results were compared with the control group, it was found to have no significant effect on each cell proliferation and apoptosis. Conversely treated with TRAIL, when treated in parallel radiation, it was possible to know that the HCT-116 cells significantly apoptosis occurs, The proportion of G1 ratio G0 also was found to have increased. TRAIL conclusion is increased apoptosis radiation defensive cells can know that increased radiosensitivity, also possible to alter the cell cycle, reduce cell proliferation ability stepwise it was possible. TRAIL is increased apoptosis, decreased cell proliferative capacity, it is considered to be possible to use as a radiation sensitizer.

주제어

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가설 설정

In this study, using the TRAIL and radiation, to determine whether the survival of the HCT-116 cells, was trying to figure out whether there is effectively anti-cancer effect.

제안 방법

^[13] While there are few side effects and the lack of effective anti-cancer therapeutics discovery and development of new therapeutic a gents has been steadily made efforts to increase the therapeutic effect. In this experiment to investigate the radiation and then further activated to induce the death of cancer cells by the immune chemicals that act only on cancer cells as the target cells to increase the radiation sensitivity at the same time evaluate use in cancer cells removed.
Processes the LmSup, LmE and the cells of the concentration of 1 × 106 cells / ml to 5 μg/ml each of the cultured HCT-116 cells, 24 hours, 48 hours, each was the control group was not treated.
Using the two methods of TRAIL and irradiation, res ult of observation of the death of cells, than single treatment of TRAIL or irradiation, treating both simultaneously have found that promoted apoptosis. Based on this, it is determined that it is possible to take advantage of the application of TRAIL as a future radiosensitizers.
Cells were possible staining with Annexin V-FITC is defined as apoptosis occurs cells were analyzed total of 10,000 cells per each sample. and activity measurement was done.

데이터처리

After 3 hours remove the MTT reagent, which was then compared to the frequency division by 100 ml of DMSO dissolved all of the formazan produced in the well using the ELISA reader (moleculer device s, sunnyvale, CA, USA) measured at 540 nm. Experimental results were the mean and standard error of three measurements. Processes the LmSup, LmE and the cells of the concentration of 1 × 10⁶ cells / ml to 5 μg/ml each of the cultured HCT-116 cells, 24 hours, 48 hours, each was the control group was not treated.

성능/효과

Along with the results of a study TRAIL of proliferative capacity of processing the TRAIL compared to the control group cells to an increase of 10,20,50,100 ng/ml, the cell proliferative capacity stepwise decrease, than 100 ng/ml statistically were significant results(Fig. 1).
environment of the cell proliferation ability By treating the TRAIL at concentrations of 20 ng / ml, the proliferative capacity of the cells was reduced by about 10% at about 90%. On the other hand, Simply a s a method of line irradiation, decreased cell proliferative capacity of about 15% at about 85%, was treated this both, proliferation ability of the cells is reduced by about 25 percent, showed a 75% survival rate It was statistic ally significant(Fig. 3).
Previous studies have been proposed in many papers that induce different responses by a variety of factors, including the type of receptor that binds to the type of target cells, the processing time. Research results of this paper, the proliferative capacity of cells treated with TRAIL as compared to the control group as the concentration of TRAIL to increase, and decreased cell proliferative capacity in stages. This is, it can be determined that the TRAIL used alone gave me to some extent reduce the cell proliferative capacity.
This is, it can be determined that the TRAIL used alone gave me to some extent reduce the cell proliferative capacity. Result of observation of the Apoptosis, according to TRAIL treatment Apoptosis show aspects that gradual increase (than 50 ng/ml of 100 ng/ml was statistically significant), which is the process of TRAIL can be viewed as induced Apoptosis. By the Comparing TRAIL and irradiation TRAIL processing (20 ng/ml), the proliferative capacity of the cells was reduced by about 10%, radiation alone enforcement is proliferative capacity of about 15% cells were decreased.
Further, the ratio of G0 Phase corresponding to the resting phase of the cell division cycle, if used in combination with TRAIL and radiation, than when carried out singly, increase the proportion of more display resting cells it TRAIL and radiation irradiation, it was possible to know the revive the action at the same time with each other. Through this experiment, when each treatment alone to TRAIL and irradiation, both were able to confirm the apoptosis, it showed Additive effect if the probability of such apoptosis is was applied to the two conditions at the same time, in the G1 phase S phase rather than move to, a change in the resting stage to the G0 phase of cell is increased cell division was found to aborted.

참고문헌 (15)

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상세보기
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TRAIL과 방사선 조사가 암세포의 사멸에 미치는 효과
TRAIL and Effect of Irradiation on Apoptosis of Cancer Cells 원문보기

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TRAIL과 방사선 조사가 암세포의 사멸에 미치는 효과 TRAIL and Effect of Irradiation on Apoptosis of Cancer Cells 원문보기

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TRAIL과 방사선 조사가 암세포의 사멸에 미치는 효과
TRAIL and Effect of Irradiation on Apoptosis of Cancer Cells 원문보기

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