[논문]한국에 서식하는 아메리카동애등에 Hermetia illucens (Diptera: Stratiomyidae)의 개체군 구조

박소연; 최한수; 최지영; 정길상

doi:10.13047/kjee.2017.31.6.520

한국에 서식하는 아메리카동애등에 Hermetia illucens (Diptera: Stratiomyidae)의 개체군 구조
Population Structure of the Exotic Black Soldier Fly, Hermetia illucens (Diptera: Stratiomyidae) in Korea 원문보기

한국환경생태학회지 = Korean journal of environment and ecology, v.31 no.6, 2017년, pp.520 - 528

박소연 (Division of Basic Research, National Institute of Ecology) , 최한수 (Institute of Ecological Engineering) , 최지영 (Rural Development Administration) , 정길상 (Division of Basic Research, National Institute of Ecology)

초록
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외래생물인 아메리카동애등에는 뛰어난 유기물분해능으로 주목받아왔다. 이에 우리는 한국내 분포상과 유전적 다양성을 알아보기 위해 전국적으로 채집하여 미토콘드리아의 사이토크롬 C 산화효소 1 (CO1) 유전자를 이용해 집단유전학분석을 실시하였다. 그 결과 이 곤충은 한국에 성공적으로 정착한 것으로 보이며 10개의 미토콘드리아 유전자형이 있었고 개체군 간 분화가 뚜렷했다. 이 결과는 국내정착한 아메리카동애등에가 특정 유전자형의 모계만이 도입된 후 지역 간 분산이 제한적임을 나타내는 것으로, 이는 이들의 단거리 비행 경향에 기인한 것으로 추정된다.

Abstract ▼ AI-Helper

The exotic black soldier fly, Hermetia illucens, has been paid much attention as an excellent organic matter decomposer. We conducted the nationwide survey and the population genetic study using a mitochondrial cytochrome C oxidase 1 gene to understand its genetic diversity and distribution pattern in Korea. The results show that it has successfully settled down in South Korea and there are only 10 haplotypes and the populations of the insect are highly differentiated. The results may indicate that their dispersal was restrained probably due to their short distance flying tendency since their introduction and settlement of only few maternal lineages with certain genotypes in Korea.

주제어

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제안 방법

The partial CO1 gene was obtained from each individual using the specific primer set developed for this study (forward: CAA CAA ATC ATA AAG ATA TTG GAA C; reverse: GTC CGA AGA ATC AAA ATA AAT GYT G).
Analysis of molecular variance (AMOVA) among regions including Fst statistics was performed for determining the population genetic structure with 1000 permutations. To test whether the sequence variation was congruent to the neutral evolution model, the neutrality tests were performed. Based on mismatch distribution, Harpending’s raggedness was estimated with 100 bootstrapping to infer the population expansion in relation to the neutrality tests.

대상 데이터

They were captured using an insect net near or on farms or organic waste stacks. The flies were collected in total 245 individuals from 30 localities nationwide during July to October in 2006, 2010 and 2011 (Table 1). Local populations were clustered into five regions based on the Korean vegetation map isothermal and isobaric lines (Yi, 2011) (Table 1).
From the 245 BSF specimens, a total of 657bp were analyzed. The sequences were deposited on GenBank (GenBank accession number: FJ794325~FJ794425, HQ541184 ~HQ541321, and KF500241~KF500278). There were 41 variable sites, and among them 35 nucleotide sites were parsimony informative.

데이터처리

Due to no HKY (Hasegawa-Kishino-Yano) option on Arlequin, K80 (Kimura 80) with the same substitution rate was used for AMOVA and Fst statistics. Recently, it was suggested that Fst did not properly measure genetic differentiation among groups (Jost, 2008).

이론/모형

5) (Librado and Rozas, 2009). Genetic distance and diversity among haplotypes were calculated after selecting the best fit substitution model for the haplotypes on MEGA. Parsimony split algorithm with 500 bootstrapping and median joining algorithm were used to infer phylogenetic relationships among the haplotypes using Splitstree (ver.
Genetic distance and diversity among haplotypes were calculated after selecting the best fit substitution model for the haplotypes on MEGA. Parsimony split algorithm with 500 bootstrapping and median joining algorithm were used to infer phylogenetic relationships among the haplotypes using Splitstree (ver. 4.12.3) (Huson and Bryant, 2006) and Network (ver. 4.6.10) (Bandelt et al., 1999) with fixed connection limit at 1000 steps between haplotypes. The haplotype networks created by the two different algorithms were manually consolidated and reconstructed with the regional distribution data on Adobe illustrator CS3 (Adobe Inc, USA).

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