[논문]Effects of high glucose with or without other metabolic substrates on alpha-adrenergic contractions in rat mesenteric and femoral arteries

Vorn, Rany; Yoo, Hae Young

doi:10.4196/kjpp.2017.21.1.91

Effects of high glucose with or without other metabolic substrates on alpha-adrenergic contractions in rat mesenteric and femoral arteries 원문보기

The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology, v.21 no.1, 2017년, pp.91 - 97

Vorn, Rany (Chung-Ang University Red Cross College of Nursing) , Yoo, Hae Young (Chung-Ang University Red Cross College of Nursing)

Abstract ▼ AI-Helper

Hyperglycemia is associated with an increased risk of cardiovascular diseases. It has been demonstrated that chronic exposure to high glucose impaired endothelial functions. However, specific effects of short-term exposure to high glucose on vascular reactivity are controversial. Moreover, the combined effects of other metabolic substrates such as free fatty acids (FFA) on vascular reactivity remain poorly understood. Here we investigate the effects of short-term exposure to high glucose with or without other metabolic substrates including FFAs termed "nutrition full" (NF) solution, on mesenteric (MA) and deep femoral arteries (DFA) of rats. Arterial ring segments were mounted in a double-wire myograph. Contraction in response to phenylephrine (PhE) was determined in control (5 mM) and high glucose (23 mM, HG) environments over a 30 min period. In both arteries, PhE-inducedvasocontraction was enhanced by pre-incubation of HG solution. A combined incubation with HG and palmitic acid ($100{\mu}M$) induced similar sensitization of PhE-contractions in both arteries. In contrast, high $K^+$-induced contractions were not affected by HG. Interestingly, pre-incubation with NF solution decreased PhE-induced contraction in MA but increased the contraction in DFA. In NF solution, the HG-induced facilitation of PhE-contraction was not observed in MA. Furthermore, the PhE-induced contraction of DFA was attenuated by HG in NF solution. Our results demonstrate that the sensitization of PhE-induced arterial contraction by HG is differentially affected by other metabolic substrates. The conversation of skeletal arterial contractility by HG in NF solution requires careful interpretation of the previous in vitro studies where only glucose is included in physiological salt solutions. Further studies are required to elucidate the mechanism underlying the inconsistent effect of NF solution on MA and DFA.

주제어

AI 본문요약
AI-Helper

* AI 자동 식별 결과로 적합하지 않은 문장이 있을 수 있으니, 이용에 유의하시기 바랍니다.

문제 정의

In addition, this is the first study to examine the effects of NF solution alone and with high glucose. These findings provide preliminary evidence for identifying the action mechanism of metabolic substrates and high glucose on vascular reactivity.

제안 방법

After confirming 80 mM KCl-induced (80K contraction) and 10 μM PhE-induced contraction, endothelium-dependent ACh-induced vasorelaxation was tested in each vessel. The tested vessels were either incubated with 23 mM glucose-containing or control PSS, and then the concentration-dependent contractile responses to PhE were analyzed. Incremental application of PhE (0.

데이터처리

The response to PhE is expressed as the percent of the second 80 mM KCl-PSS-induced maximum constriction. Half-maximal effective concentrations (EC₅₀ levels) were calculated by non linear regression analyses of the concentration response curves generated with OriginPro 8.0 software (OriginLab, Northampton, MA, USA). Statistical analysis was performed with the unpaired Student’s t-test.
Statistical analysis was performed with the unpaired Student’s t-test.

성능/효과

In conclusion, the results of the current study demonstrated that short-term exposure to a high glucose solution augmented sensitivity to PhE-induced contraction in rat MAs and DFAs. In addition, this is the first study to examine the effects of NF solution alone and with high glucose.
The results of the present study showed that only 30 min of incubation in HG enhances the sensitivity to PhE-induced contraction in rat MAs and DFAs. More interesting finding was that the incubation in NF solution induces opposite responses, decrease and increase of PhE-contractions in MAs and DFAs, respectively.

후속연구

Conventional in vitro studies of arterial contractility is conducted in physiological salt solutions with glucose only for the metabolic substrates. Considering the importance of vascular functions in the pathophysiology of metabolic diseases, it is requested to have a comprehensive understanding of systemic arterial contractility under physiological conditions. On these backgrounds, we investigate the effects of short-term incubation with high glucose with or without other metabolic substrates on α-adrenergic agonist induced contraction in rat mesenteric arteries (MAs) and deep femoral arteries (DFAs).
Previous studies showed that individual free fatty acids have similar effects in MAs and DFAs, and endothelial cell dysfunction was induced with high and physiological concentrations of both saturated and unsaturated fatty acids [21,36]. Further investigation is needed to identify these inconsistent effects of NF solutions using various arteries and solution compositions.

참고문헌 (36)

1 Fowler MJ Microvascular and macrovascular complications of diabetes Clinical Diabetes 2008 26 77 82
2 Triggle CR Samuel SM Ravishankar S Marei I Arunachalam G Ding H The endothelium: influencing vascular smooth muscle in many ways Can J Physiol Pharmacol 2012 90 713 738 22625870

상세보기
3 Sandoo A van Zanten JJ Metsios GS Carroll D Kitas GD The endothelium and its role in regulating vascular tone Open Cardiovasc Med J 2010 4 302 312 21339899

상세보기
4 Rajendran P Rengarajan T Thangavel J Nishigaki Y Sakthisekaran D Sethi G Nishigaki I The vascular endothelium and human diseases Int J Biol Sci 2013 9 1057 1069 24250251

상세보기
5 Taylor PD Poston L The effect of hyperglycaemia on function of rat isolated mesenteric resistance artery Br J Pharmacol 1994 113 801 808 7858870

상세보기
6 Akbari CM Saouaf R Barnhill DF Newman PA LoGerfo FW Veves A Endothelium-dependent vasodilatation is impaired in both microcirculation and macrocirculation during acute hyperglycemia J Vasc Surg 1998 28 687 694 9786265
7 Brouwers O Niessen PM Haenen G Miyata T Brownlee M Stehouwer CD De Mey JG Schalkwijk CG Hyperglycaemia-induced impairment of endothelium-dependent vasorelaxation in rat mesenteric arteries is mediated by intracellular methylglyoxal levels in a pathway dependent on oxidative stress Diabetologia 2010 53 989 1000 20186387

상세보기
8 Zhou ZW Xie XL Zhou SF Li CG Mechanism of reversal of high glucose-induced endothelial nitric oxide synthase uncoupling by tanshinone IIA in human endothelial cell line EA.hy926 Eur J Pharmacol 2012 697 97 105 23063542

상세보기
9 Koo JR Ni Z Oviesi F Vaziri ND Antioxidant therapy potentiates antihypertensive action of insulin in diabetic rats Clin Exp Hypertens 2002 24 333 344 12109774

상세보기
10 Marfella R Quagliaro L Nappo F Ceriello A Giugliano D Acute hyperglycemia induces an oxidative stress in healthy subjects J Clin Invest 2001 108 635 636 11518739

상세보기
11 Li PA Liu GJ He QP Floyd RA Siesjo BK Production of hydroxyl free radical by brain tissues in hyperglycemic rats subjected to transient forebrain ischemia Free Radic Biol Med 1999 27 1033 1040 10569636

상세보기
12 Hsieh TJ Zhang SL Filep JG Tang SS Ingelfinger JR Chan JS High glucose stimulates angiotensinogen gene expression via reactive oxygen species generation in rat kidney proximal tubular cells Endocrinology 2002 143 2975 2985 12130563

상세보기
13 Li H Chai Q Gutterman DD Liu Y Elevated glucose impairs cAMP-mediated dilation by reducing Kv channel activity in rat small coronary smooth muscle cells Am J Physiol Heart Circ Physiol 2003 285 H1213 H1219 12763748

상세보기
14 El-Awady MS El-Agamy DS Suddek GM Nader MA Propolis protects against high glucose-induced vascular endothelial dysfunction in isolated rat aorta J Physiol Biochem 2014 70 247 254 24234058
15 MacKenzie A Cooper EJ Dowell FJ Differential effects of glucose on agonist-induced relaxations in human mesenteric and subcutaneous arteries Br J Pharmacol 2008 153 480 487 18037911

상세보기
16 Williams SB Goldfine AB Timimi FK Ting HH Roddy MA Simonson DC Creager MA Acute hyperglycemia attenuates endothelium-dependent vasodilation in humans in vivo Circulation 1998 97 1695 1701 9591763

상세보기
17 Chinen I Shimabukuro M Yamakawa K Higa N Matsuzaki T Noguchi K Ueda S Sakanashi M Takasu N Vascular lipotoxicity: endothelial dysfunction via fatty-acid-induced reactive oxygen species overproduction in obese Zucker diabetic fatty rats Endocrinology 2007 148 160 165 17023526

상세보기
18 Nishikawa T Edelstein D Du XL Yamagishi S Matsumura T Kaneda Y Yorek MA Beebe D Oates PJ Hammes HP Giardino I Brownlee M Normalizing mitochondrial superoxide production blocks three pathways of hyperglycaemic damage Nature 2000 404 787 790 10783895

상세보기
19 Saraswathi V Wu G Toborek M Hennig B Linoleic acid-induced endothelial activation: role of calcium and peroxynitrite signaling J Lipid Res 2004 45 794 804 14993245

상세보기
20 Jaimes EA Hua P Tian RX Raij L Human glomerular endothelium: interplay among glucose, free fatty acids, angiotensin II, and oxidative stress Am J Physiol Renal Physiol 2010 298 F125 F132 19864304

상세보기
21 Sainsbury CA Sattar N Connell JM Hillier C Petrie JR Non-esterified fatty acids impair endothelium-dependent vasodilation in rat mesenteric resistance vessels Clin Sci (Lond) 2004 107 625 629 15367101
22 Bhagavan NV Ha JS Park JH Honda SA Rios CN Sugiyama C Fujitani GK Takeuchi IK Ha CE Utility of serum Fatty Acid concentrations as a marker for acute myocardial infarction and their potential role in the formation of ischemia-modified albumin: a pilot study Clin Chem 2009 55 1588 1590 19498051

상세보기
23 Banskota AH Tezuka Y Kadota S Recent progress in pharmacological research of propolis Phytother Res 2001 15 561 571 11746834
24 Qian L Wang H Xia Q Bruce I Huang H Interleukin-2 improves vascular functions in streptozotocin-induced diabetic rats 2005 IEEE Engineering in Medicine and Biology 27th Annual Conference 2005 17-18 Jan 2006
25 Paneni F Beckman JA Creager MA Cosentino F Diabetes and vascular disease: pathophysiology, clinical consequences, and medical therapy: part I Eur Heart J 2013 34 2436 2443 23641007

상세보기
26 Gerich JE Clinical significance, pathogenesis, and management of postprandial hyperglycemia Arch Intern Med 2003 163 1306 1316 12796066

상세보기
27 Endemann DH Schiffrin EL Endothelial dysfunction J Am Soc Nephrol 2004 15 1983 1992 15284284

상세보기
28 Beckman JA Goldfine AB Gordon MB Creager MA Ascorbate restores endothelium-dependent vasodilation impaired by acute hyperglycemia in humans Circulation 2001 103 1618 1623 11273987

상세보기
29 Ahmad M Turkseven S Mingone CJ Gupte SA Wolin MS Abraham NG Heme oxygenase-1 gene expression increases vascular relaxation and decreases inducible nitric oxide synthase in diabetic rats Cell Mol Biol (Noisy-le-grand) 2005 51 371 376 16309587
30 Ozkan MH Uma S Inhibition of acetylcholine-induced EDHF response by elevated glucose in rat mesenteric artery Life Sci 2005 78 14 21 16125203
31 Rajagopalan S Kurz S Munzel T Tarpey M Freeman BA Griendling KK Harrison DG Angiotensin II-mediated hypertension in the rat increases vascular superoxide production via membrane NADH/NADPH oxidase activation. Contribution to alterations of vasomotor tone J Clin Invest 1996 97 1916 1923 8621776
32 Inoguchi T Li P Umeda F Yu HY Kakimoto M Imamura M Aoki T Etoh T Hashimoto T Naruse M Sano H Utsumi H Nawata H High glucose level and free fatty acid stimulate reactive oxygen species production through protein kinase C--dependent activation of NAD(P)H oxidase in cultured vascular cells Diabetes 2000 49 1939 1945 11078463

상세보기
33 Inoguchi T Sonta T Tsubouchi H Etoh T Kakimoto M Sonoda N Sato N Sekiguchi N Kobayashi K Sumimoto H Utsumi H Nawata H Protein kinase C-dependent increase in reactive oxygen species (ROS) production in vascular tissues of diabetes: role of vascular NAD(P)H oxidase J Am Soc Nephrol 2003 14 8 Suppl 3 S227 S232 12874436
34 Tomasian D Keaney JF Vita JA Antioxidants and the bioactivity of endothelium-derived nitric oxide Cardiovasc Res 2000 47 426 435 10963716

상세보기
35 Kipnes M Dandona P Tripathy D Still JG Kosutic G Control of postprandial plasma glucose by an oral insulin product (HIM2) in patients with type 2 diabetes Diabetes Care 2003 26 421 426 12547873

상세보기
36 Lundman P Tornvall P Nilsson L Pernow J A triglyceride-rich fat emulsion and free fatty acids but not very low density lipoproteins impair endothelium-dependent vasorelaxation Atherosclerosis 2001 159 35 41 11689204

상세보기

LOADING...

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증