메기, Silurus asotus의 외과적 시술시 상처 회복 및 스트레스 반응을 알아보기 위하여, 수술 과정에 의한 스트레스 호르몬의 변화 양상과 회복과정을 분석하였다. 수술 후 clove oil (1,000 ppm) 마취한 군의 생존율은 수술 후 3~42일 동안 약 90%를 보였으며 무마취군은 약 74%를 보였다. 즉, 마취를 한 후 수술하였을 때 무마취군 보다 생존율이 높게 나타났다. Clove oil로 마취한 군의 혈장 cortisol 농도가 무마취군 보다 높았으며(P<0.05) 아울러, clove oil로 마취한 군의 혈장 lactic acid 농도 역시 무마취군 보다 높았다(P<0.05). 수술 후 14일과 28일째는 아직 봉합사의 흔적이 남아 있었고 움직임도 활동적이지 않았지만 35일째 되었을 때는 봉합자국은 사라졌고 수술 후 42일에는 봉합사도 관찰되지 않았으며, 봉합 상처도 회복이 되었다. 따라서 메기의 외과시술시, 마취를 한 후 수술하였을 때 무마취군 보다 생존율이 높게 나타났고, 수술 후 받는 스트레스 양도 적다는 것을 알 수 있다. 즉, 본 연구의 메기에서의 외과적 상처 회복은 마취제 사용시 성공적으로 이루어짐을 보였다.
메기, Silurus asotus의 외과적 시술시 상처 회복 및 스트레스 반응을 알아보기 위하여, 수술 과정에 의한 스트레스 호르몬의 변화 양상과 회복과정을 분석하였다. 수술 후 clove oil (1,000 ppm) 마취한 군의 생존율은 수술 후 3~42일 동안 약 90%를 보였으며 무마취군은 약 74%를 보였다. 즉, 마취를 한 후 수술하였을 때 무마취군 보다 생존율이 높게 나타났다. Clove oil로 마취한 군의 혈장 cortisol 농도가 무마취군 보다 높았으며(P<0.05) 아울러, clove oil로 마취한 군의 혈장 lactic acid 농도 역시 무마취군 보다 높았다(P<0.05). 수술 후 14일과 28일째는 아직 봉합사의 흔적이 남아 있었고 움직임도 활동적이지 않았지만 35일째 되었을 때는 봉합자국은 사라졌고 수술 후 42일에는 봉합사도 관찰되지 않았으며, 봉합 상처도 회복이 되었다. 따라서 메기의 외과시술시, 마취를 한 후 수술하였을 때 무마취군 보다 생존율이 높게 나타났고, 수술 후 받는 스트레스 양도 적다는 것을 알 수 있다. 즉, 본 연구의 메기에서의 외과적 상처 회복은 마취제 사용시 성공적으로 이루어짐을 보였다.
To find out the stress response and wound healing of surgical incisions, we analyzed the change of stress hormone and the healing process in far eastern catfish, Silurus asotus. The survival rate of the clove oil (1,000 ppm) anesthesia group after surgery was about 90% in 3~42 days, while no anesthe...
To find out the stress response and wound healing of surgical incisions, we analyzed the change of stress hormone and the healing process in far eastern catfish, Silurus asotus. The survival rate of the clove oil (1,000 ppm) anesthesia group after surgery was about 90% in 3~42 days, while no anesthesia group showed about 74%. Thus, the survival rate after anesthesia group was higher than that of the no anesthesia group. The plasma cortisol concentration of the clove oil anesthesia group was higher than that of the no anesthesia group (P<0.05). In addition, the plasma glucose concentration of clove oil anesthesia group was higher than that of the no anesthesia group (P<0.05). The plasma lactic acid concentration of clove oil anesthetized group was higher than that of the not anesthetized group (P<0.05). On the 14 days and 28 days after surgery, there were still stitching fiber of suture and swimming behavior was not active. On the 35 days after surgery, trace of the suture has almost disappeared on the outside of the fish. Finally, 42 days after surgery, stitching fiber was not visible, and the sutured wounds were distinctly recovered. The results of this study showed that the anesthesia group showed higher survival rate and received less postoperative stress than that of the no anesthesia group. Surgical wound healing in the catfish of this study was shown to be successful when using anesthetics.
To find out the stress response and wound healing of surgical incisions, we analyzed the change of stress hormone and the healing process in far eastern catfish, Silurus asotus. The survival rate of the clove oil (1,000 ppm) anesthesia group after surgery was about 90% in 3~42 days, while no anesthesia group showed about 74%. Thus, the survival rate after anesthesia group was higher than that of the no anesthesia group. The plasma cortisol concentration of the clove oil anesthesia group was higher than that of the no anesthesia group (P<0.05). In addition, the plasma glucose concentration of clove oil anesthesia group was higher than that of the no anesthesia group (P<0.05). The plasma lactic acid concentration of clove oil anesthetized group was higher than that of the not anesthetized group (P<0.05). On the 14 days and 28 days after surgery, there were still stitching fiber of suture and swimming behavior was not active. On the 35 days after surgery, trace of the suture has almost disappeared on the outside of the fish. Finally, 42 days after surgery, stitching fiber was not visible, and the sutured wounds were distinctly recovered. The results of this study showed that the anesthesia group showed higher survival rate and received less postoperative stress than that of the no anesthesia group. Surgical wound healing in the catfish of this study was shown to be successful when using anesthetics.
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제안 방법
Blood samples were extracted from five randomly selected fish, using syringes lined with the anticoagulant heparin. Blood was extracted from five experimental samples, at fixed intervals of pre (0), 1, 6, 12, 24, 48, 72, and 96 hours after surgery. The collected blood was placed in capillary tubes, and analyzed, after centrifugation at 200 × g for 10 min.
During the experimental period, five experimental fishes were sampled and anesthetized, and a picture taken of the surgical site; and the surgical site was extracted, and was fixed in 10% neutral formalin (100 mL formalin, 6.5g Na2HPO4 · 12H2O, 4.5 g KH2PO4, 900 mL DW) for 24 hours, with sampling being executed at 7 days intervals, for 42 days after surgery.
, 1994). In this study, the stress response by surgical surgery investigated the cellular reaction and neuroendocrine, and their interaction. Primarily, the activity of the hypothalamus - pituitary is high, which causes the secretion of cortisol in the blood (Chang and Hur, 1999).
Using scissors for surgery, the surgical site, including the epidermis and the muscles, were cut deeply, and the length of incision was about a 2~3 cm wound, parallel to the sideline. The wounded samples were sutured using suture needle (Ophthalmic suture needle No. 0, Ailee, Korea), and a simple-instrument method of incision with 3~4 stitches. To complete, the surgical site was sutured using a brush, and the wound was applied with vaseline (Vaseline intensive care, Unilever, USA).
대상 데이터
Blood samples were extracted from five randomly selected fish, using syringes lined with the anticoagulant heparin. Blood was extracted from five experimental samples, at fixed intervals of pre (0), 1, 6, 12, 24, 48, 72, and 96 hours after surgery.
Far eastern catfish, Silurus asotus, were obtained from the Fisheries Genetics and Breeding Sciences Laboratory, Korea Maritime and Ocean University, Busan, Korea. Their average total length was 35.
데이터처리
The differences among groups were analyzed by ANOVA, using the SPSS statistics package (SPSS 9.0, SPSS Inc., Chicago, IL, USA); and multiple comparisons were performed, using Duncan’s multiple range test.
성능/효과
The healing began to progress towards adhesion at 7 days after surgery, and the movement of fishes at 7 days after surgery was more active, than that at 1 day after surgery. At 14 days after surgery, 52% of all samples were observed with slight adhesion, and the ratio of slight adhesion increased until 28 days after surgery. At 28 days after surgery, all samples were observed with slight adhesion, and due to the lack of epidermal melanin, traces of off-white appeared in the surgical site.
At 28 days after surgery, all samples were observed with slight adhesion, and due to the lack of epidermal melanin, traces of off-white appeared in the surgical site. At 35 days after surgery, 52% of all samples were observed with substantial adhesion as complete adhesions in the surgical site, and all experimental samples were completely adhered at 42 days after surgery.
Fifteen far eastern catfish, Silurus asotus, of the not anesthetized group died after surgery, and the survival rate of this group gradually decreased, until 7 days after surgery. The survival rate of the not anesthetized group was maintained at 74%, from 8 days to 42 days after surgery (Table 1).
They can remove or separate the necrotic tissue, fill the defect site due to wound, or eliminate foreign material, the original components of which are fibroblasts (Marty and Summerfelt, 1990). This study, to externally target far eastern catfish, and the histological findings of the present investigation 35 days after surgery, shows the surgical site was healing almost 100% after 42 days after surgery, showing recovery. These results suggest that compared with Marty and Summerfelt (1990) in the channel catfish, Ictalurus punctaus, complete abdominal wound healing when compared with 93 days, and healing of far eastern catfish were faster, than that of channel catfish.
후속연구
The water temperature is an important factor in wound healing, due to the experimental fish temperature effects on wound healing, Anderson and Roberts (1975) reported the temperature effects of wound healing on White Cloud Mountain fish, Tanichthys albonubes, and Roubal and Bullock (1988) investigated the effect of hydrocortison on the wound healing of Atlantic salmon, Salmo salar. Our study is also associated with the future of these two complementary experimental diets, and indicates further research will be required. In this study, the results of a survey on the healing process from the outside of the wound of the surgical site are given.
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