[논문]Molecular Typing and Antimicrobial Susceptibility of Staphylococcus aureus Strains Isolated from Raw Milk, Cheese, Minced Meat, and Chicken Meat Samples

Can, Hayriye Yesim; Elmali, Mehmet; Karagoz, Alper

doi:10.5851/kosfa.2017.37.2.175

Molecular Typing and Antimicrobial Susceptibility of Staphylococcus aureus Strains Isolated from Raw Milk, Cheese, Minced Meat, and Chicken Meat Samples 원문보기

Korean journal for food science of animal resources = 한국축산식품학회지, v.37 no.2, 2017년, pp.175 - 180

Can, Hayriye Yesim (Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, Mustafa Kemal University) , Elmali, Mehmet (Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, Mustafa Kemal University) , Karagoz, Alper (Microbiology Reference Laboratories Department, Public Health Institutes of Turkey)

Abstract ▼ AI-Helper

The objectives of this study were: i) to detect the presence of Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) in raw milk, cheese, beef minced meat, and chicken meat samples; ii) to evaluate the antimicrobial susceptibility of the isolates; and iii) to determine clonal relation among the isolates by using pulsed-field gel electrophoresis (PFGE) method. Therefore, a total of 160 food samples were randomly collected between August 2014 and May 2015 in Hatay province, located in the southern Turkey. Twenty (12.5%) of the samples were found to be contaminated with S. aureus. A total of 40 isolates from the 20 positive samples were confirmed to be S. aureus by multiplex PCR based on 16S rRNA and nuc gene. The mec A gene was not detected in any of the S. aureus strains. In the present study, 39 out of 40 (97.5%) isolates were found to be resistant to one or more antibiotics. All of isolates were susceptible to gentamicin, oxacillin, and vancomycin. The highest resistance rate was detected in penicillin (95%) and ampicillin (92.5%), followed by tetracycline (30%), erythromycin (20%), ciprofloxacin (12.5%). Nine major patterns were determined by PFGE. In 6 of these patterns, thirty-six strains (90%) had identical PFGE profiles.

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문제 정의

aureus is available in Turkey. The aims of this study were to evaluate the presence of S. aureus and MRSA in various food samples, and to determine the antimicrobial susceptibility and clonal relationships of the isolates.

제안 방법

A multiplex PCR assay was performed in a reaction mixture of 25 μL final volume containing 1×PCR buffer (Thermoscientific, Lithuania), 1.5 mM MgCI2 (Thermoscientific), 200 μM of each dNTPs (EURx, Poland), 1 U Taq DNA polymerase (Thermoscientific), 0.12 μM of each primer, and 2 μL template DNA.
All coagulase positive isolates were identified by species- specific PCR. In this study, multiplex PCR assay was based on the detection of 16S rRNA (Staphylococcus genus specific), and nuc gene (S. aureus species specific). Methicillin resistance was also determined by testing for the presence of mecA gene.

대상 데이터

A total of 160 samples were randomly collected between August 2014 and May 2015 in Hatay province, located in the southern Turkey. Samples including 50 raw milk (20 cows’, 15 goats’, and 15 ewes’ bulk milk), 50 traditional cheeses (25 Carra, 25 Surk), 30 chicken meat (wing and drumsticks with skin), and 30 beef minced meat were transported to the laboratory with ice packs for analysis.
Samples including 50 raw milk (20 cows’, 15 goats’, and 15 ewes’ bulk milk), 50 traditional cheeses (25 Carra, 25 Surk), 30 chicken meat (wing and drumsticks with skin), and 30 beef minced meat were transported to the laboratory with ice packs for analysis.

이론/모형

aureus strains was also performed. SmaI restriction enzyme was used in PFGE method. Nine major patterns were determined in 40 strains.

참고문헌 (28)

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연합인증

Molecular Typing and Antimicrobial Susceptibility of Staphylococcus aureus Strains Isolated from Raw Milk, Cheese, Minced Meat, and Chicken Meat Samples 원문보기

Abstract ▼ AI-Helper

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Molecular Typing and Antimicrobial Susceptibility of Staphylococcus aureus Strains Isolated from Raw Milk, Cheese, Minced Meat, and Chicken Meat Samples 원문보기

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참고문헌 (28)

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