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dsRNA를 이용한 해충방제 기술
Insect Pest Control Technique Using dsRNA 원문보기

한국응용곤충학회지 = Korean journal of applied entomology, v.56 no.2, 2017년, pp.153 - 164  

김용균 (안동대학교 식물의학과)

초록
AI-Helper 아이콘AI-Helper

이중나선형 RNA (dsRNA)를 이용한 유전자 발현 억제기술이 다양한 생명체에서 기능 유전체학을 연구하는 데 이용되고 있다. 이 기술의 원리는 전사후 단계에서 유전자 발현을 조절하는 RNA 간섭에 기인된다. dsRNA를 이용하여 특정 유전자의 발현 억제는 심각한 치사효과를 줄 수 있다. 이러한 분자기작을 해충 방제에 적용하여 특정 dsRNA를 이용한 새로운 살충제를 개발하고 있다. 본 종설은 dsRNA를 이용한 RNA간섭 원리를 설명하고 이를 이용한 해충 방제를 구현한 여러 예를 살펴본다. 그리고 해충방제를 실현시키기 위해 현재 이 기술이 담고 있는 한계를 고찰하고 이에 대한 대응 방안을 본 종설에서 제공하고자 한다.

Abstract AI-Helper 아이콘AI-Helper

Gene silencing using double-stranded RNA (dsRNA) has been widely used in functional genomics in biological organisms. Its principle stems from RNA interference (RNAi), a post-transcriptional control of gene expression. Suppression of specific gene expression using dsRNA may give significant lethal e...

주제어

질의응답

핵심어 질문 논문에서 추출한 답변
RNAi는 어떻게 나뉘는가? RNAi는 세포 자율적(cell-autonomous) RNAi와 세포 비자율적(non-cell-autonomous) RNAi로 나뉘게 된다(Whangboand Hunter, 2008). 세포 자율적 RNAi는 세포 스스로 형성한 dsRNA 또는 외부로부터 주입된 dsRNA에 의해 자체 세포내에서만 RNAi 과정이 국한될 경우이다.
RNA 간섭은 무엇인가? 여기에 분자 크기가 작은 small RNA가 추가된다. RNA 간섭은 micro RNA (miRNA)와 같은 small RNA에 의해 상보적인 mRNA의 발현을 억제하는 현상으로 2006년 AndrewFire와 Craig Mello가 노벨 생리의학상을 수상하면서 다양한분야의 과학자들로부터 관심을 받게 되었다. 또 다른 축의 RNA 간섭 현상은 dsRNA에 반응하여 유기되는 과정이다.
sRNA를 발현하는 형질전환 작물을 제조하는 방식은 무엇으로 불리는가? 셋째로 dsRNA를 발현하는 형질전환 작물을 제조하는 방식이다. 이 기술을 특별히 식물유래(in planta) RNAi로 불린다. 이 식물유래 RNAi에서 dsRNA는 앞에서 기술한 바와 같이 머리핀 모양으로 발현시키게 된다.
질의응답 정보가 도움이 되었나요?

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