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Reproductive & developmental biology = 한국동물번식학회지, v.41 no.1, 2017년, pp.17 - 23
이원희 (강원대학교 동물생명과학대학) , 박지은 (강원대학교 동물생명과학대학) , 황보용 (강원대학교 동물생명과학대학) , 김화영 (강원대학교 동물생명과학대학) , 이지은 (강원대학교 동물생명과학대학) , 강병범 (강원대학교 동물생명과학대학) , 정희태 (강원대학교 수의과대학) , 양부근 (강원대학교 동물생명과학대학) , 박춘근 (강원대학교 동물생명과학대학)
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The oocyte undergoes various events during in vitro maturation (IVM) and subsequence development. One of the events is production of reactive oxygen species (ROS) that is a normal process of cell metabolism. But imbalances between ROS production and antioxidant systems induce oxidative stress that n...
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| 핵심어 | 질문 | 논문에서 추출한 답변 |
|---|---|---|
| 가축산업에서 배아의 체외 생산 기술이 이용되는 분야는? | 가축산업에 있어 배아의 체외 생산 기술은 중요한 기술 중 하나로 여겨지고 있으며, 특히 유전물질의 분배와종의 보존, 가축의 대량생산, biomedical animal 등 다양한 분야에 이용되고 있다(Appeltant 등, 2015). 특히 돼지는 인간과 장기의 성질과 크기가 비슷하기 때문에(Kwak등, 2012), 이종 장기 이식 동물 또는 인간의 질병 모델개발을 위한 형질전환동물 분야에서 많이 연구되고 있다(Jeon 등, 2014). | |
| 형질전환동물 연구 시, 돼지를 많이 활용하는 이유는? | 가축산업에 있어 배아의 체외 생산 기술은 중요한 기술 중 하나로 여겨지고 있으며, 특히 유전물질의 분배와종의 보존, 가축의 대량생산, biomedical animal 등 다양한 분야에 이용되고 있다(Appeltant 등, 2015). 특히 돼지는 인간과 장기의 성질과 크기가 비슷하기 때문에(Kwak등, 2012), 이종 장기 이식 동물 또는 인간의 질병 모델개발을 위한 형질전환동물 분야에서 많이 연구되고 있다(Jeon 등, 2014). 다양한 분야에서 사용되는 배아를 성공적으로 체외 생산하기 위해 체외성숙, 수정 및 배양 과정을 거치게 되지만, 체내보다 높은 산소의 농도, 빛의 노출, 시약 또는 배양액의 오염, 연구자의 미숙한 기량 등체외 환경에 의해 세포내에서 생성된 활성산소종(reactive oxygen species, ROS)으로 인해 손상 받게 된다. | |
| 세포 내 산화스트레스를 유발하는 활성산소종의 종류는? | 다양한 분야에서 사용되는 배아를 성공적으로 체외 생산하기 위해 체외성숙, 수정 및 배양 과정을 거치게 되지만, 체내보다 높은 산소의 농도, 빛의 노출, 시약 또는 배양액의 오염, 연구자의 미숙한 기량 등체외 환경에 의해 세포내에서 생성된 활성산소종(reactive oxygen species, ROS)으로 인해 손상 받게 된다. Hydrogen peroxide(H2O2), superoxide(O2—), hydroxyl radi- cal(•OH) 등을 포함하고 있는 ROS는 세포 내 산화스트레스를 유발한다고 알려져 있으며, 난자의 성숙과 발달을 저해한다(Kitagawa 등, 2004). 체외환경에서 생산된 세포내 ROS로 인하여 체외 생산된 배아는 체내 유래 수정란보다 그 품질이 저하된다고 알려져 있으며, 난자의 체외성숙과 배아발달 동안 산화스트레스를 감소시키기 위해 세포내에서 배양액에 항산화제(Spinaci 등, 2008) 및 비타민(Tareq 등, 2012) 등을 첨가한 연구가 많이 진행되고 있다. |
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