In this study, we investigated the effects of light spectra on physiology stress and DNA damage in juvenile rock bream (Oplegnathus fasciatus) using light-emitting diodes (LEDs; green, 520 nm; red, 630 nm) at two intensities (0.25 and $0.5W/m^2$ ) with application of thermal stress (25 an...
In this study, we investigated the effects of light spectra on physiology stress and DNA damage in juvenile rock bream (Oplegnathus fasciatus) using light-emitting diodes (LEDs; green, 520 nm; red, 630 nm) at two intensities (0.25 and $0.5W/m^2$ ) with application of thermal stress (25 and $30^{\circ}C$). We measured the mRNA expression of heat shock protein 70 (HSP70) and the levels of plasma cortisol, glucose, aspartate aminotransferase (AspAT), and alanine aminotransferase (AlaAT). Additionally, DNA damage was measured using comet assays. Our findings showed that HSP70 mRNA expression and plasma cortisol, glucose, AspAT, and AlaAT levels were significantly higher after exposure to high temperatures and were significantly lower after exposure to green LED light. Thus, although high water temperatures induced stress in juvenile rock bream, green LED light inhibited stress. In particular, green LED light reduced stress and DNA damage to a greater degree than other light sources.
In this study, we investigated the effects of light spectra on physiology stress and DNA damage in juvenile rock bream (Oplegnathus fasciatus) using light-emitting diodes (LEDs; green, 520 nm; red, 630 nm) at two intensities (0.25 and $0.5W/m^2$ ) with application of thermal stress (25 and $30^{\circ}C$). We measured the mRNA expression of heat shock protein 70 (HSP70) and the levels of plasma cortisol, glucose, aspartate aminotransferase (AspAT), and alanine aminotransferase (AlaAT). Additionally, DNA damage was measured using comet assays. Our findings showed that HSP70 mRNA expression and plasma cortisol, glucose, AspAT, and AlaAT levels were significantly higher after exposure to high temperatures and were significantly lower after exposure to green LED light. Thus, although high water temperatures induced stress in juvenile rock bream, green LED light inhibited stress. In particular, green LED light reduced stress and DNA damage to a greater degree than other light sources.
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제안 방법
In this study, we also investigated changes in cortisol and glucose concentrations, which are controlled by the synthesis and release of HPI axis activity, in order to investigate the stress response induced by the high temperature environment. Our results showed that cortisol and glucose concentrations differed according to light source, with significant increases upon exposure to green LED light compared with that in the control group.
In this study, we performed comet assays to measure the degree of nuclear DNA damage in the liver cells of juvenile rock bream following exposure to high water temperatures. Notably, our results showed that high water temperatures caused nuclear DNA damage in liver cells.
The calibrated ΔCt value (ΔΔCt) per sample and that for their internal control (β-actin) were calculated as follows: (ΔΔCt = 2−[ΔCtsample − ΔCtinternal control]). The qPCR data from three replicate samples were analyzed using a CFX96 Real-Time System (Bio-Rad) to estimate the transcript copy numbers in each sample.
대상 데이터
7 g) were purchased from a commercial aquarium (Jeju, Korea) and were allowed to acclimate in ten 100-L circulation filter tanks in the laboratory. Each tank (each experimental group) contained 15 fish. The fish in the control group were exposed to a white fluorescent bulb.
1. Spectral profiles of the light-emitting diodes (LEDs; green, 520 nm; red, 630 nm) and white fluorescent bulb (Cont.) used in this study. Each LED light source was set to two intensities: low, 0.
데이터처리
One-way analysis of variance (ANOVA) followed by Tukey’s posthoc test was used to compare differences in the data (P <0.05).
이론/모형
Absorbance was read at 450 nm. Plasma AspAT/AlaAT and glucose levels were measured using a dry multiplayer analytic slide method in a biochemistry auto analyzer (Fuji Dri-Chem 4000; Fujifilm, Tokyo, Japan).
성능/효과
Moreover, cortisol and glucose concentrations in fish exposed to the green LED light are significantly lower when compared with those in groups exposed to other types of light (fluorescent bulbs, blue, red, and purple LEDs). Consistent with these findings, our results showed that the cortisol and glucose concentration were significantly increased when rock bream were exposed to a high-temperature environment, although the green LED light effectively reduced the stress generated in the fish body.
In conclusion, the results of this study suggested that the green LED light reduced environmental stress and nuclear DNA damage in juvenile rock bream and effectively mitigated water temperature stress, even when used at a low intensity (0.25 W/m2). In contrast, the red LED light caused stress and nuclear DNA damage, with increased alterations observed as the intensity of light increased.
(2014) reported that when goldfish are exposed to high temperatures, AspAT and AlaAT concentrations are increased significantly as the water temperature increases. Our results also showed that the green LED light reduced thermal-induced liver damage, as result decreased AspAT and AlaAT concentrations in the plasma, even for the lower light intensity (0.25 W/m2).
In this study, we also investigated changes in cortisol and glucose concentrations, which are controlled by the synthesis and release of HPI axis activity, in order to investigate the stress response induced by the high temperature environment. Our results showed that cortisol and glucose concentrations differed according to light source, with significant increases upon exposure to green LED light compared with that in the control group. However, cortisol and glucose concentrations were decreased following exposure to red light.
(2014) observed that as the temperature increased, the expression of HSP70 mRNA increased. Our results, which are consistent with the previous findings, suggested that HSP70 mRNA expression was significantly increased when rock bream was exposed to a high temperature environment and that green LED light effectively reduced the expression of HSP70 mRNA, regardless of the intensity of light. However, red LED light was found to increase the expression of HSP70 mRNA.
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