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Identification of Genes Related to Fungicide Resistance in Fusarium fujikuroi 원문보기

Mycobiology, v.45 no.2, 2017년, pp.101 - 104  

Choi, Younghae (Department of Applied Biology, Dong-A University) ,  Jung, Boknam (Department of Applied Biology, Dong-A University) ,  Li, Taiying (Department of Applied Biology, Dong-A University) ,  Lee, Jungkwan (Department of Applied Biology, Dong-A University)

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We identified two genes related to fungicide resistance in Fusarium fujikuroi through random mutagenesis. Targeted gene deletions showed that survival factor 1 deletion resulted in higher sensitivity to fungicides, while deletion of the gene encoding F-box/WD-repeat protein increased resistance, sug...

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  • PCR was performed with three arbitrary degenerate primers (R1, R2, and R3) and three specific primers (SP1-6) designed from vector sequences introduced into the mutant strains (Table 1). After the third PCR, the amplicons were cloned into the pGEM-T Easy vector (Promega, Madison, WI, USA) for sequencing and the sequences analyzed using the Basic Local Alignment Search Tool (BLAST; National Center for Biotechnology Information, Bethesda, MD, USA) using the blastn algorithm (Table 2). Fungicide resistance in 5 of the 7 mutants was mitotically instable, as consecutive regeneration resulted in the loss of fungicide resistance.
  • Phylogenetic analyses of survival factor 1 (A) and F-box/WD-repeat protein (B) in Fusarium fujikuroi and other known fungal species. The amino acid sequences were aligned with ClustalW, and MEGA software ver. 6.0 was used to perform 1,000 bootstrap phylogenetic analyses using the neighbor-joining method.
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참고문헌 (14)

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  4. 4 Jeon YA Yu SH Lee YY Park HJ Lee S Sung JS Kim YG Lee HS Incidence, molecular characteristics and pathogenicity of Gibberella fujikuroi species complex associated with rice seeds from Asian countries Mycobiology 2013 41 225 233 24493944 

  5. 5 Kim SH Park MR Kim YC Lee SW Choi BR Lee SW Kim IS Degradation of prochloraz by rice bakanae disease pathogen Fusarium fujikuroi with differing sensitivity: a possible explanation for resistance mechanism J Korean Soc Appl Biol Chem 2010 53 433 439 

  6. 6 Shin MU Kang HJ Lee YH Kim HT Detection for the resistance of Fusarium spp. Isolated from rice seeds to prochloraz and cross-resistance to other fungicides inhibiting sterol biosynthesis Korean J Pestic Sci 2008 12 277 282 

  7. 7 Youn K Choi HW Shin DB Jung B Lee J Resistance of Fusarium fujikuroi isolates to hydrogen peroxide and its application for fungal isolation Res Plant Dis 2015 21 227 230 

  8. 8 Cappellini RA Peterson JL Macroconidium formation in submerged cultures by a non-sporulating strain of Gibberella zeae Mycologia 1965 57 962 966 

  9. 9 Horwitz BA Sharon A Lu SW Ritter V Sandrock TM Yoder OC Turgeon BG A G protein alpha subunit from Cochliobolus heterostrophus involved in mating and appressorium formation Fungal Genet Biol 1999 26 19 32 10072317 

  10. 10 Kim JE Jin J Kim H Kim JC Yun SH Lee YW GIP2, a putative transcription factor that regulates the aurofusarin biosynthetic gene cluster in Gibberella zeae Appl Environ Microbiol 2006 72 1645 1652 16461721 

  11. 11 Liu YG Whittier RF Thermal asymmetric interlaced PCR: automatable amplification and sequencing of insert end fragments from P1 and YAC clones for chromosome walking Genomics 1995 25 674 681 7759102 

  12. 12 Lee J Leslie JF Bowden RL Expression and function of sex pheromones and receptors in the homothallic ascomycete Gibberella zeae Eukaryot Cell 2008 7 1211 1221 18503004 

  13. 13 Amatulli MT Spadaro D Gullino ML Garibaldi A Molecular identification of Fusarium spp. associated with bakanae disease of rice in Italy and assessment of their pathogenicity Plant Pathol 2010 59 839 844 

  14. 14 Yu JH Hamari Z Han KH Seo JA Reyes-Domínguez Y Scazzocchio C Double-joint PCR: a PCR-based molecular tool for gene manipulations in filamentous fungi Fungal Genet Biol 2004 41 973 981 15465386 

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