The effects of slaughter age (28, 32, or 38 mon) on the proximate composition, collagen content, fatty acid composition, amino acid content, and mineral contents of horse leg bone (femur and tibia) extracts (HLBE) obtained from Jeju crossbred horses (n=14) in Korea. HLBE was extracted for 24 h with ...
The effects of slaughter age (28, 32, or 38 mon) on the proximate composition, collagen content, fatty acid composition, amino acid content, and mineral contents of horse leg bone (femur and tibia) extracts (HLBE) obtained from Jeju crossbred horses (n=14) in Korea. HLBE was extracted for 24 h with boiling water. At those ages, the respective proximate compositions of the HLBE were found as follows: 5.20-6.42% crude protein; fat 0.61-1.65% crude; and 0.10-0.22% crude ash. At 32 and 38 mon, the HLBE showed higher levels of both crude protein and collagen than at 28 mon. The major fatty acids of the horse leg bone extract powder (HLBP) were palmitic acid and oleic acid. Palmitoleic acid and the essential fatty acids were higher in the HLBP at 38 mon compared to that at 28 mon. Nearly all the amino acids were found at higher levels in the HLBP at 38 mon than at 28 mon, except histidine. The P, K, Zn, Se and Fe contents in the HLBP increased significantly with age. These results suggest that some nutrients in the HLBE increase with age, and that extracts from horses older than 32 mon would be more nutritious for human consumers.
The effects of slaughter age (28, 32, or 38 mon) on the proximate composition, collagen content, fatty acid composition, amino acid content, and mineral contents of horse leg bone (femur and tibia) extracts (HLBE) obtained from Jeju crossbred horses (n=14) in Korea. HLBE was extracted for 24 h with boiling water. At those ages, the respective proximate compositions of the HLBE were found as follows: 5.20-6.42% crude protein; fat 0.61-1.65% crude; and 0.10-0.22% crude ash. At 32 and 38 mon, the HLBE showed higher levels of both crude protein and collagen than at 28 mon. The major fatty acids of the horse leg bone extract powder (HLBP) were palmitic acid and oleic acid. Palmitoleic acid and the essential fatty acids were higher in the HLBP at 38 mon compared to that at 28 mon. Nearly all the amino acids were found at higher levels in the HLBP at 38 mon than at 28 mon, except histidine. The P, K, Zn, Se and Fe contents in the HLBP increased significantly with age. These results suggest that some nutrients in the HLBE increase with age, and that extracts from horses older than 32 mon would be more nutritious for human consumers.
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대상 데이터
The femur and tibia bones were obtained and kept in a freezer until analysis. Horse leg bone extracts (HLBE) and horse leg bone extracts powder (HLBP) were prepared as shown in Fig. 1. To clean the bones, the femur and tibia were soaked in water overnight at 4°C, and the water was discarded.
The decomposed samples were vacuum evaporated in a 55°C water bath (JSWB-22T, JS Research Inc., Korea).
데이터처리
For the statistical analysis of all the data, one-way analysis of variance (ANOVA) calculations were performed using the General Linear Model (GLM) procedure in the SAS software package (ver. 9, SAS Institute Inc., USA) for all variables considered in the study. When treatment effects were significant (p<0.
When treatment effects were significant (p<0.05), mean values were compared with Duncan’s multiple range test.
이론/모형
Amino acid analysis was conducted according to the Aristoy and Toldra (1991)'s method with slight modification.
To determine the proximate composition of HLBE, the analyses were performed using AOAC methods (AOAC International, 1997). Crude protein was assessed using the Kjeldahl method (Method 726.08); crude fat was determined by solvent extraction (Method 991.36); and moisture was obtained by oven drying (Method 950.46). Crude ash, as the inorganic matter in the sample, was analyzed from dry ashing using furnace (Method 920.
To determine the proximate composition of HLBE, the analyses were performed using AOAC methods (AOAC International, 1997). Crude protein was assessed using the Kjeldahl method (Method 726.
후속연구
Despite the prior lack of nutritional information for Jeju crossbred horses, our results show that bone extracts from horses at 38 mon of age are more nutritious than those from 28 and 32 monold horses, since they contain significantly more nutrients due to their high levels of palmitoleic acid, essential amino acids, minerals, and collagen. Further in vivo tests are needed to evaluate the bioavailability of these nutrients.
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