Objective: This work was to find the correlation of alcohol dehydrogenase 1C (ADH1C) genotype with vitamin A reduction and carcass traits during the vitamin A restriction period. Methods: In study 1, 60 Korean native steers were fed a diet (890 IU/kg) with 8,000 IU and 0 IU of supplemental premix vi...
Objective: This work was to find the correlation of alcohol dehydrogenase 1C (ADH1C) genotype with vitamin A reduction and carcass traits during the vitamin A restriction period. Methods: In study 1, 60 Korean native steers were fed a diet (890 IU/kg) with 8,000 IU and 0 IU of supplemental premix vitamin A/kg of dry matter (DM) for control and treatment group, respectively. The levels of serum vitamin A were analyzed through high preparative performance liquid chromatography, and the ADH1C genotype was analyzed based on polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP; 78.1% TT type, 21.9% TC type); however, CC type was not found. Then, the interaction between ADH1C and carcass traits on the vitamin A restriction was investigated in study 2. A total of 136 Korean native steers were fed a diet that included 930 IU/kg vitamin A of DM. Results: Serum vitamin A in treatment was reduced to 112.4 IU/dL in steers with TT type of ADH1C, while for steers with TC type the concentration of serum vitamin A was dropped to 79.5 IU/dL (p<0.1) in study 1. This showed that TC type had the potential to lower serum vitamin A concentration during vitamin A restriction compared to TT type. In study 2 we found that eye muscle area, marbling and carcass weight in Korean native steers with TC type were higher than in steers with TT type (p<0.05). Conclusion: The interaction between vitamin A restriction and TC type of ADH1C gene could have the potential of increasing the marbling in Korean native steers. These results indicated that steers with TC type of the ADH1C gene were more sensitive to the change of serum vitamin A than TT types. Furthermore, this finding has the potential to enable a higher marbling score under the condition of vitamin A restriction in Korean native steers.
Objective: This work was to find the correlation of alcohol dehydrogenase 1C (ADH1C) genotype with vitamin A reduction and carcass traits during the vitamin A restriction period. Methods: In study 1, 60 Korean native steers were fed a diet (890 IU/kg) with 8,000 IU and 0 IU of supplemental premix vitamin A/kg of dry matter (DM) for control and treatment group, respectively. The levels of serum vitamin A were analyzed through high preparative performance liquid chromatography, and the ADH1C genotype was analyzed based on polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP; 78.1% TT type, 21.9% TC type); however, CC type was not found. Then, the interaction between ADH1C and carcass traits on the vitamin A restriction was investigated in study 2. A total of 136 Korean native steers were fed a diet that included 930 IU/kg vitamin A of DM. Results: Serum vitamin A in treatment was reduced to 112.4 IU/dL in steers with TT type of ADH1C, while for steers with TC type the concentration of serum vitamin A was dropped to 79.5 IU/dL (p<0.1) in study 1. This showed that TC type had the potential to lower serum vitamin A concentration during vitamin A restriction compared to TT type. In study 2 we found that eye muscle area, marbling and carcass weight in Korean native steers with TC type were higher than in steers with TT type (p<0.05). Conclusion: The interaction between vitamin A restriction and TC type of ADH1C gene could have the potential of increasing the marbling in Korean native steers. These results indicated that steers with TC type of the ADH1C gene were more sensitive to the change of serum vitamin A than TT types. Furthermore, this finding has the potential to enable a higher marbling score under the condition of vitamin A restriction in Korean native steers.
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가설 설정
During the fattening period, vitamin A restriction was conducted in treatment by feeding the feedstuff which contained 0.89 IU/g vitamin A in study 1. The concentration of vitamin A in the control group was at a high level (8.89 IU/g). At the beginning of the experimental period, the concentration of serum in treatment group and control group were in the similar level (p>0.
대상 데이터
The groups contained 8,000 IU and 0 IU of supplemental premix vitamin A/kg of dry matter (DM), respectively. The experiment was conducted during the fattening period in Korean native steers (from 15 to 24 months of age), and the composition of feedstuff nutrient in study 1 is shown in Table 1. Blood samples were collected from steers for each month during the period of the experiment.
데이터처리
The concentration of serum and genotype were in included in one-way ANOVA test, and the genotype×vitamin A interaction were included in two-way ANOVA test.
The data of carcass traits were analyzed through Duncan’s multiple range tests using a one-way ANOVA in SPSS Version 21 for Windows (SPSS Inc., USA).
The data of serum vitamin A were analyzed by Duncan’s multiple range test using one-way and two-way analysis of variance (one-way and two-way ANOVA) in SPSS Version 21 for Windows (SPSS Inc., Chicago, IL, USA).
이론/모형
The DNA was extracted from beef using the manual method. Beef was cut into small slices and placed into a 1.
성능/효과
During the experimental period, concentration of serum vitamin A in the treatment group was decreased significantly during the experiment period (p<0.05) in Table 2. The level of serum vitamin A in the control group did not show any change by feeding with a high level of vitamin A (8.89 IU/g). Previous studies also showed that serum vitamin A was reduced without the supplement of vitamin A in the diet [8,9].
In conclusion, TC type of ADH1C gene tended to reach lower serum vitamin A level during the vitamin A restriction period in Korean native steers. At the same time, when vitamin A was restricted in Korean native steers, the steers in TC type of ADH1C gene had a much higher level of intramuscular fat than the steers in TT type.
However, we didn’t find any CC type in the 60 steers. In study 2, the ratio of TT type is 75%, with 25% of 136 Korean native steers TC type, and we still did not find the CC type of ADH1C gene. Totally we found 78% TT type and 22% TC type in these steers which was showed in Table 3.
참고문헌 (19)
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