The objective of this study was to evaluate the antioxidant and anti-aging effects of marigold methanol extract (MGME) in human dermal fibroblasts. Total polyphenolic and flavonoid contents in MGME were 74.8 mg TAE (tannic acid equivalent)/g and 85.6 mg RE (rutin equivalent)/g, respectively. MGME (<...
The objective of this study was to evaluate the antioxidant and anti-aging effects of marigold methanol extract (MGME) in human dermal fibroblasts. Total polyphenolic and flavonoid contents in MGME were 74.8 mg TAE (tannic acid equivalent)/g and 85.6 mg RE (rutin equivalent)/g, respectively. MGME ($500{\mu}g/mL$) increased 1,1-diphenyl-2-picryl hydrazyl (DPPH) and 2,2'-azino-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical-scavenging, and superoxide dismutase (SOD)-like antioxidant activities by 36.5, 54.7, and 14.8%, respectively, compared with the control. At $1,000{\mu}g/mL$, these activities increased by 63.7, 70.6, and 20.6%, respectively. MGME ($100{\mu}g/mL$) significantly increased the synthesis of type 1 procollagen by 83.7% compared with control treatment. It also significantly decreased Matrix Metalloproteinase-2 (MMP-2) activity and MMP-1 mRNA expression by 36.5% and 69.5%, respectively; however, it significantly increased laminin-5 mRNA expression by 181.2%. These findings suggest that MGME could protect human skin against photo-aging by attenuating oxidative damage, suppressing MMP expression and/or activity as well as by stimulating collagen synthesis.
The objective of this study was to evaluate the antioxidant and anti-aging effects of marigold methanol extract (MGME) in human dermal fibroblasts. Total polyphenolic and flavonoid contents in MGME were 74.8 mg TAE (tannic acid equivalent)/g and 85.6 mg RE (rutin equivalent)/g, respectively. MGME ($500{\mu}g/mL$) increased 1,1-diphenyl-2-picryl hydrazyl (DPPH) and 2,2'-azino-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical-scavenging, and superoxide dismutase (SOD)-like antioxidant activities by 36.5, 54.7, and 14.8%, respectively, compared with the control. At $1,000{\mu}g/mL$, these activities increased by 63.7, 70.6, and 20.6%, respectively. MGME ($100{\mu}g/mL$) significantly increased the synthesis of type 1 procollagen by 83.7% compared with control treatment. It also significantly decreased Matrix Metalloproteinase-2 (MMP-2) activity and MMP-1 mRNA expression by 36.5% and 69.5%, respectively; however, it significantly increased laminin-5 mRNA expression by 181.2%. These findings suggest that MGME could protect human skin against photo-aging by attenuating oxidative damage, suppressing MMP expression and/or activity as well as by stimulating collagen synthesis.
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제안 방법
Cytotoxicity of MGME against human dermal fibroblasts was determined by the MTT assay, and the MPL was determined to be 100 μg/mL.
In this study, we evaluate the oxidant and anti-aging effects of marigold methanol extract (MGME) by examining collagen synthesis and its degradation, as wells as by examining laminin synthesis in human dermal fibroblasts.
Morphological changes were examined and photographed with an Olympus BX51 microscope (Olympus, Tokyo, Japan) and ProRes® C14 plus charge-coupled device camera (Jenoptik Optical Systems, Tokyo, Japan).
대상 데이터
Dimethyl sulfoxide (DMSO), 1,1-diphenyl-2-picryl hydrazyl (DPPH), 2,2'-azino-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), tannic acid, ascorbic acid, Folin-Ciocalteu’s phenol reagent, and 3- (4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) were obtained from Sigma-Aldrich (St. Louis, MO, UK).
Methanol extract of the marigold plant (Tagetes erecta L.) was obtained from the Korea Plant Extract Bank (Daejeon, Korea). Whole T.
Cytotoxicity of MGME against human dermal fibroblasts was determined by the MTT assay, and the MPL was determined to be 100 μg/mL. The dermis contains 85~90% type 1 collagen. Procollagen (collagen precursor) is synthesized in dermal fibroblasts in the form of polypeptide chains of type 1 collagen (34).
데이터처리
The difference in values between groups was statistically evaluated with one-way analyses of variance (ANOVA) followed by post-hoc Duncan multiple range tests using SPSS 18.0 statistical software (SPSS Inc., Chicago, IL, USA). Differences were considered statistically significant for p < 0.
이론/모형
5% agarose gel and visualized with ethidium bromide staining. The DNA band densities were evaluated with the KODAK Gel Logic 100 image analysis system (Eastman Kodak Co., Rochester, NY, USA).
A standard curve was prepared using tannic acid. The total flavonoid content of the MGME was determined by a modified Davis method (18). The MGME (100 μL) was placed in a test tube before adding 1 mL of diethylene glycol reagent and 100 μL of 1 N NaOH.
성능/효과
In addition, at these concentrations, MMP-1 transcript levels were significantly (p < 0.05) reduced in a dose-dependent manner by 50.3%, 51.5%, 60.2%, and 69.5%, respectively, as determined by RT-PCR (Fig. 6).
In this study, MGME (1,000 μg/mL) exhibited free radical-scavenging capacities of 63.7% (DPPH assay) and 70.6% (ABTS assay).
In this study, MGME (100 μg/mL) significantly decreased MMP-2 activity by 36.5% in human dermal fibroblasts compared with control treatment.
In this study, MGME (100 μg/mL) significantly decreased the expression of MMP-1 mRNA by 69.5% in human dermal fibroblasts compared with control treatment.
In this study, collagen production significantly increased by 83.7% in MGME (100 μg/ mL)-treated fibroblasts compared to that in control fibroblasts, indicating that MGME is a potent inducer of collagen synthesis.
In this study, laminin-5 expression significantly increased by 181.2% in MGME (100 μg/mL)-treated human dermal fibroblasts compared to that in vehicle-treated fibroblasts, indicating that MGME holds remarkable skin anti-aging potential.
Results of this study indicate that MGME, owing to the high levels of polyphenols, induces collagen synthesis and inhibits its degradation in human dermal fibroblasts. These findings suggest that MGME could protect human skin against photo-aging by suppressing MMP expression and/ or activity as well as by stimulating collagen synthesis.
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