Sasa quelpaertensis Nakai leaves contain a mixture of polysaccharides, amino acids, and polyphenols, which mediate various biological activities. For efficient utilization of its leaf, we reported the preparation procedure for phytochemical-rich extract (PRE) using the leaf residue, which was by-pro...
Sasa quelpaertensis Nakai leaves contain a mixture of polysaccharides, amino acids, and polyphenols, which mediate various biological activities. For efficient utilization of its leaf, we reported the preparation procedure for phytochemical-rich extract (PRE) using the leaf residue, which was by-product of hot water extraction. This study was undertaken to evaluate the effects of PRE and its major constituent, p-coumaric acid,on the growth of several human cancer cell lines (MKN-74, MKN-45, SNU-1, SNU-16, and HL-60). The ethyl acetate fraction of PRE and p-coumaric acid significantly inhibited the proliferation of MKN-74 and HL-60 cells, respectively, and induced cell apoptosis, down-regulated Bcl-2 and poly (ADP-ribose) polymerase levels, and up-regulated those of Bax and caspase-3. These results show the potential utility of S. quelpaertensis Nakai leaves in cancer prevention.
Sasa quelpaertensis Nakai leaves contain a mixture of polysaccharides, amino acids, and polyphenols, which mediate various biological activities. For efficient utilization of its leaf, we reported the preparation procedure for phytochemical-rich extract (PRE) using the leaf residue, which was by-product of hot water extraction. This study was undertaken to evaluate the effects of PRE and its major constituent, p-coumaric acid,on the growth of several human cancer cell lines (MKN-74, MKN-45, SNU-1, SNU-16, and HL-60). The ethyl acetate fraction of PRE and p-coumaric acid significantly inhibited the proliferation of MKN-74 and HL-60 cells, respectively, and induced cell apoptosis, down-regulated Bcl-2 and poly (ADP-ribose) polymerase levels, and up-regulated those of Bax and caspase-3. These results show the potential utility of S. quelpaertensis Nakai leaves in cancer prevention.
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제안 방법
In this study, we evaluated the cell growth inhibition activity of PRE fractions and p-coumaric acid using four human gastric cancer cell lines (MKN-74, MKN-45, SNU-16, and SNU-1 cell) and acute leukemia cell line (HL-60 cell). Hexane, chloroform, ethylacetate and butanol fractions of PRE inhibited the proliferation of these cells to differing extents (Fig.
13-14 However, the effects of PRE and p-coumaric on human cancer cells, and the underlying molecular mechanisms of action, are not yet completely understood. In this study, we investigated the effects of the ethyl acetate fraction of PRE (EPRE) and p-coumaric on the proliferation of several human cancer cell types.
Induction of apoptosis by EPRE and p-coumaric acid was evaluated using flow cytometry by measuring the amount of sub-G1 DNA. EPRE and p-coumaric acid induced apoptosis in HL-60 cells (Fig.
데이터처리
Values are expressed as means ± standard deviation (SD). One-way analysis of variance (ANOVA) was used for multiple comparisons. Treatment effects were analyzed using Duncan’s multiple range test, performed with SPSS software (ver.
Treatment effects were analyzed using Duncan’s multiple range test, performed with SPSS software (ver. 12.0; SPSS Inc., Chicago, IL, USA).
이론/모형
Cell viability assay – Cell viability was determined using MTT assay.
후속연구
The relative expression levels of pro- and anti-apoptotic proteins are key factors in the inhibitory effects of EPRE and p-coumaric acid. Further studies on the anticancer properties of EPRE and p-coumaric acid could further reveal the potential of S. quelpaertensis leaves in cancer prevention.
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